Recombinant TRF2 Antibody

    Application:
  • IF/ICC
  • WB
Features and benefits
  • Host species: Rabbit
  • Species reactivity(Reacts with): Human
  • Isotype: Rabbit IgG
  • Conjugation: Unconjugated
Item Number
Ab132424
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab132424-10μl
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$69.90
Ab132424-50μl
50μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$199.90
Ab132424-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$309.90
Ab132424-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,699.90

Recombinant; Rabbit anti Human TRF2 Antibody; WB, IF, ICC; Unconjugated

Basic Description

Product NameRecombinant TRF2 Antibody
SynonymsTelomeric DNA-binding protein | telomeric repeat binding factor 2 | Telomeric repeat binding protein 2 | telomeric repeat-binding factor 2 | TERF2 | TRBF2 | TRF2 | TRF-2 | TTAGGG repeat binding factor 2 | TTAGGG repeat-binding factor 2 | Telomeric DNA bin
Specifications & PurityExactAb™, Validated, Recombinant, Lot by Lot
Host speciesRabbit
SpecificityTERF2
ImmunogenA synthetic peptide derived from human TERF2 (AA 50-100)
Positive ControlWB: HeLa, HEK293 and K562 cell lysates. IF/ICC: HeLa cells.
ConjugationUnconjugated
GradeExactAb™, Recombinant, Validated
Product Description

Rabbit anti Human TRF2 Antibody, Recombinant, could be used for WB, IF, ICC and so on.
Application:
WB: 1/1000-1/2000
ICC/IF: 1/50-1/200
Protein Function:
Binds the telomeric double-stranded 5'-TTAGGG-3' repeat and plays a central role in telomere maintenance and protection against end-to-end fusion of chromosomes. In addition to its telomeric DNA-binding role, required to recruit a number of factors and enzymes required for telomere protection, including the shelterin complex, TERF2IP/RAP1 and DCLRE1B/Apollo. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded 5'-TTAGGG-3' repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. Together with DCLRE1B/Apollo, plays a key role in telomeric loop (T loop) formation by generating 3' single-stranded overhang at the leading end telomeres: T loops have been proposed to protect chromosome ends from degradation and repair. Required both to recruit DCLRE1B/Apollo to telomeres and activate the exonuclease activity of DCLRE1B/Apollo. Preferentially binds to positive supercoiled DNA. Together with DCLRE1B/Apollo, required to control the amount of DNA topoisomerase (TOP1, TOP2A and TOP2B) needed for telomere replication during fork passage and prevent aberrant telomere topology. Recruits TERF2IP/RAP1 to telomeres, thereby participating in to repressing homology-directed repair (HDR), which can affect telomere length.

Product Properties

IsotypeRabbit IgG
Light Chain Typekappa
SDS-PAGE150 kDa
Purification MethodProtein A purified
FormLiquid
ConcentrationLot by Lot
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Recombinant TRF2 Antibody (Ab132424) - Western Blot
All lanes: Recombinant TRF2 Antibody (Ab132424) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/40000 dilution

Predicted band size: 60 kDa
Observed band size: 65 - 67 kDa
The expression profile and molecular weight observed may be due to post-translational modifications.
Exposure time: 28.8 s

Recombinant TRF2 Antibody (Ab132424) - IF/ICC
Immunofluorescent analysis of HeLa cells, using the Recombinant TRF2 Antibody (Ab132424)  at 1/150 dilution.

Associated Targets(Human)

TERF2 Tchem Telomeric repeat-binding factor 2 (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

Mechanisms of Action

Mechanism of ActionAction Typetarget IDTarget NameTarget TypeTarget OrganismBinding Site NameReferences

Application

ApplicationDilution info
IF/ICC 1/50-1/200

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1/50-1/200

WB 1/1000-1/2000

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1/1000-1/2000

Certificates

Certificate of Analysis(COA)

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Related Documents

Solution Calculators