| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab132484-10μl | 10μl | In stock | $66.90 | |
| Ab132484-50μl | 50μl | In stock | $189.90 | |
| Ab132484-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $299.90 | |
| Ab132484-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,799.90 |
Recombinant; Rabbit anti Human TRIM25 Antibody; WB, ICC, IF; Unconjugated
| Product Name | Recombinant TRIM25 Antibody - Primary antibody, specific to TRIM25, Rabbit IgG |
|---|---|
| Synonyms | 2.3.2.27 | EC 6.3.2.n3 | Estrogen-responsive finger protein | RING finger protein 147 | RING-type E3 ubiquitin transferase | RING-type E3 ubiquitin transferase TRIM25 | Tripartite motif-containing protein 25 | Ubiquitin/ISG15-conjugating enzyme TRIM25 | Z |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 0.5 mg/mL |
| Host species | Rabbit |
| Specificity | TRIM25 |
| Immunogen | A synthetic peptide derived from human TRIM25 (AA 142-157) |
| Positive Control | WB: K562, HeLa and C6 cell lysates. ICC/IF: MCF7 cells. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description | Rabbit anti Human TRIM25 Antibody, Recombinant, could be used for WB, ICC, IF and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Antigen affinity purified |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant TRIM25 Antibody (Ab132484) - Western Blot
All lanes: Recombinant TRIM25 Antibody (Ab132484) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 71 kDa
Observed band size: 69 kDa
Exposure time: 17.0 s
Recombinant TRIM25 Antibody (Ab132484) - Western Blot
All lanes: Recombinant TRIM25 Antibody (Ab132484) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 71 kDa
Observed band size: 69 kDa
Exposure time: 1.0 s
Recombinant TRIM25 Antibody (Ab132484) - IF/ICC
Immunocytochemistry analysis of TRIM25/EFP (green) in MCF7 using Recombinant TRIM25 Antibody (Ab132484), and cells were counterstained with DAPI (blue).
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000"> 1/500-1/1000 |
| IF/ICC |
1/50-1/200
">
1/50-1/200
|
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0102399 | Certificate of Analysis | Jan 10, 2024 | Ab132484 |
| ZJ24F0102398 | Certificate of Analysis | Jan 10, 2024 | Ab132484 |
| 1. Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M. (2009) Lysine acetylation targets protein complexes and co-regulates major cellular functions.. Science, 325 (5942): (834-40). [PMID:19608861] [10.1021/op500134e] |
| 2. Inoue, S S and 9 more authors.. (1993) Genomic binding-site cloning reveals an estrogen-responsive gene that encodes a RING finger protein.. Proceedings of the National Academy of Sciences of the United States of America, (1): [PMID:8248217] |
| 3. Shimada, Norihiro N and 8 more authors.. (2004) Systemic distribution of estrogen-responsive finger protein (Efp) in human tissues.. Molecular and cellular endocrinology, (15): [PMID:15130519] |
| 4. Rush, John J and 9 more authors.. (2005) Immunoaffinity profiling of tyrosine phosphorylation in cancer cells.. Nature biotechnology, [PMID:15592455] |
| 5. Zody, Michael C MC and 73 more authors.. (2006) DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage.. Nature, (20): [PMID:16625196] |
| 6. Nakasato, Norie N and 5 more authors.. (2006) A ubiquitin E3 ligase Efp is up-regulated by interferons and conjugated with ISG15.. Biochemical and biophysical research communications, (15): [PMID:17069755] |
| 7. Zou, Weiguo W, Wang, Ji J and Zhang, Dong-Er DE.. (2007) Negative regulation of ISG15 E3 ligase EFP through its autoISGylation.. Biochemical and biophysical research communications, (2): [PMID:17222803] |
| 8. Gack, Michaela U MU and 10 more authors.. (2007) TRIM25 RING-finger E3 ubiquitin ligase is essential for RIG-I-mediated antiviral activity.. Nature, (19): [PMID:17392790] |
| 9. Gack, Michaela Ulrike MU and 9 more authors.. (2009) Influenza A virus NS1 targets the ubiquitin ligase TRIM25 to evade recognition by the host viral RNA sensor RIG-I.. Cell host & microbe, (8): [PMID:19454348] |
| 10. Rigbolt, Kristoffer T G KT and 9 more authors.. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.. Science signaling, (15): [PMID:21406692] |
| 11. Dong, Xue-Yuan XY and 5 more authors.. (2012) Oestrogen causes ATBF1 protein degradation through the oestrogen-responsive E3 ubiquitin ligase EFP.. The Biochemical journal, (15): [PMID:22452784] |
| 12. Oshiumi, Hiroyuki H, Miyashita, Moeko M, Matsumoto, Misako M and Seya, Tsukasa T.. (2013) A distinct role of Riplet-mediated K63-Linked polyubiquitination of the RIG-I repressor domain in human antiviral innate immune responses.. PLoS pathogens, [PMID:23950712] |
| 13. Santiago, Felix W FW and 9 more authors.. (2014) Hijacking of RIG-I signaling proteins into virus-induced cytoplasmic structures correlates with the inhibition of type I interferon responses.. Journal of virology, [PMID:24478431] |
| 14. Koliopoulos, Marios G MG, Esposito, Diego D, Christodoulou, Evangelos E, Taylor, Ian A IA and Rittinger, Katrin K.. (2016) Functional role of TRIM E3 ligase oligomerization and regulation of catalytic activity.. The EMBO journal, (1): [PMID:27154206] |
| 15. Sanchez, Jacint G JG and 8 more authors.. (2016) Mechanism of TRIM25 Catalytic Activation in the Antiviral RIG-I Pathway.. Cell reports, (2): [PMID:27425606] |
| 16. Li, Melody M H MM and 11 more authors.. (2017) TRIM25 Enhances the Antiviral Action of Zinc-Finger Antiviral Protein (ZAP).. PLoS pathogens, [PMID:28060952] |
| 17. Zheng, Xiaojiao X and 5 more authors.. (2017) TRIM25 Is Required for the Antiviral Activity of Zinc Finger Antiviral Protein.. Journal of virology, (1): [PMID:28202764] |
| 18. Lian, Huan H and 12 more authors.. (2018) The Zinc-Finger Protein ZCCHC3 Binds RNA and Facilitates Viral RNA Sensing and Activation of the RIG-I-like Receptors.. Immunity, (18): [PMID:30193849] |
| 19. Ban, Junsu J and 5 more authors.. (2018) Human Respiratory Syncytial Virus NS 1 Targets TRIM25 to Suppress RIG-I Ubiquitination and Subsequent RIG-I-Mediated Antiviral Signaling.. Viruses, (14): [PMID:30558248] |
| 20. Chen, Szu-Ting and 15 more authors.. (2019) NLRP12 Regulates Anti-viral RIG-I Activation via Interaction with TRIM25.. Cell host & microbe, (10): [PMID:30902577] |
