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Recombinant TRIM25 Antibody - Primary antibody, specific to TRIM25, Rabbit IgG

ExactAb™
Recombinant
Validated
0.5 mg/mL

Features and benefits

Short Overview:
Recombinant; Rabbit anti Human TRIM25 Antibody; WB, ICC, IF; Unconjugated
Species reactivity(Reacts with): Human,Rat
Isotype: Rabbit IgG

Application:

ICC/IF
WB

Item Number

Ab132484

Grouped product items
SKU	Size	Availability	Price
Ab132484-10μl	10μl	In stock	$66.90
Ab132484-50μl	50μl	In stock	$189.90
Ab132484-100μl	100μl	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$299.90
Ab132484-1ml	1ml	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$2,799.90

Recombinant; Rabbit anti Human TRIM25 Antibody; WB, ICC, IF; Unconjugated

View related series

Accession#:Q14258 Gene ID:7706 TRIM25

Basic Description

Product Name	Recombinant TRIM25 Antibody - Primary antibody, specific to TRIM25, Rabbit IgG
Synonyms	E3 ubiquitin/ISG15 ligase TRIM25 antibody; EFP antibody; Estrogen responsive finger protein antibody; Estrogen-responsive finger protein antibody; RING finger protein 147 antibody; RNF 147 antibody; RNF147 antibody; TRI25 antibody; TRI25_HUMAN antibody; T
Specifications & Purity	ExactAb™, Validated, Recombinant, 0.5 mg/mL
Host species	Rabbit
Specificity	TRIM25
Immunogen	A synthetic peptide derived from human TRIM25 (AA 142-157)
Positive Control	WB: K562, HeLa and C6 cell lysates. ICC/IF: MCF7 cells.
Conjugation	Unconjugated
Grade	ExactAb™, Recombinant, Validated
Product Description	Rabbit anti Human TRIM25 Antibody, Recombinant, could be used for WB, ICC, IF and so on. Application: WB: 1/500-1/1000 ICC/IF: 1/50-1/200 Protein Function Functions as an ubiquitin E3 ligase and as an ISG15 E3 ligase. Involved in innate immune defense against viruses by mediating ubiquitination of DDX58. Mediates 'Lys-63'-linked polyubiquitination of the DDX58 N-terminal CARD-like region which is crucial for triggering the cytosolic signal transduction that leads to the production of interferons in response to viral infection. Promotes ISGylation of 14-3-3 sigma (SFN), an adapter protein implicated in the regulation of a large spectrum signaling pathway. Mediates estrogen action in various target organs.

Product Properties

Isotype	Rabbit IgG
SDS-PAGE	150 kDa
Purification Method	Antigen affinity purified
Form	Liquid
Concentration	0.5 mg/mL
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Recombinant TRIM25 Antibody (Ab132484) - Western Blot
All lanes: Recombinant TRIM25 Antibody (Ab132484) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 71 kDa
Observed band size: 69 kDa
Exposure time: 17.0 s

Recombinant TRIM25 Antibody (Ab132484) - IF/ICC
Immunocytochemistry analysis of TRIM25/EFP (green) in MCF7 using Recombinant TRIM25 Antibody (Ab132484), and cells were counterstained with DAPI (blue).

Application

Dilution info

1/500-1/1000

ICC/IF

1/50-1/200

Certificates

Certificate of Analysis(COA)

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2 results found

Lot Number	Certificate Type	Date	Item
ZJ24F0102399	Certificate of Analysis	Jan 10, 2024	Ab132484
ZJ24F0102398	Certificate of Analysis	Jan 10, 2024	Ab132484

References

1. Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M. (2009) Lysine acetylation targets protein complexes and co-regulates major cellular functions.. Science, 325 (5942): (834-40). [PMID:19608861]
2. Inoue, S S and 9 more authors.. (1993) Genomic binding-site cloning reveals an estrogen-responsive gene that encodes a RING finger protein.. Proceedings of the National Academy of Sciences of the United States of America, (1): [PMID:8248217]
3. Shimada, Norihiro N and 8 more authors.. (2004) Systemic distribution of estrogen-responsive finger protein (Efp) in human tissues.. Molecular and cellular endocrinology, (15): [PMID:15130519]
4. Rush, John J and 9 more authors.. (2005) Immunoaffinity profiling of tyrosine phosphorylation in cancer cells.. Nature biotechnology, [PMID:15592455]
5. Zody, Michael C MC and 73 more authors.. (2006) DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage.. Nature, (20): [PMID:16625196]
6. Nakasato, Norie N and 5 more authors.. (2006) A ubiquitin E3 ligase Efp is up-regulated by interferons and conjugated with ISG15.. Biochemical and biophysical research communications, (15): [PMID:17069755]
7. Zou, Weiguo W, Wang, Ji J and Zhang, Dong-Er DE.. (2007) Negative regulation of ISG15 E3 ligase EFP through its autoISGylation.. Biochemical and biophysical research communications, (2): [PMID:17222803]
8. Gack, Michaela U MU and 10 more authors.. (2007) TRIM25 RING-finger E3 ubiquitin ligase is essential for RIG-I-mediated antiviral activity.. Nature, (19): [PMID:17392790]
9. Gack, Michaela Ulrike MU and 9 more authors.. (2009) Influenza A virus NS1 targets the ubiquitin ligase TRIM25 to evade recognition by the host viral RNA sensor RIG-I.. Cell host & microbe, (8): [PMID:19454348]
10. Rigbolt, Kristoffer T G KT and 9 more authors.. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.. Science signaling, (15): [PMID:21406692]
11. Dong, Xue-Yuan XY and 5 more authors.. (2012) Oestrogen causes ATBF1 protein degradation through the oestrogen-responsive E3 ubiquitin ligase EFP.. The Biochemical journal, (15): [PMID:22452784]
12. Oshiumi, Hiroyuki H, Miyashita, Moeko M, Matsumoto, Misako M and Seya, Tsukasa T.. (2013) A distinct role of Riplet-mediated K63-Linked polyubiquitination of the RIG-I repressor domain in human antiviral innate immune responses.. PLoS pathogens, [PMID:23950712]
13. Santiago, Felix W FW and 9 more authors.. (2014) Hijacking of RIG-I signaling proteins into virus-induced cytoplasmic structures correlates with the inhibition of type I interferon responses.. Journal of virology, [PMID:24478431]
14. Koliopoulos, Marios G MG, Esposito, Diego D, Christodoulou, Evangelos E, Taylor, Ian A IA and Rittinger, Katrin K.. (2016) Functional role of TRIM E3 ligase oligomerization and regulation of catalytic activity.. The EMBO journal, (1): [PMID:27154206]
15. Sanchez, Jacint G JG and 8 more authors.. (2016) Mechanism of TRIM25 Catalytic Activation in the Antiviral RIG-I Pathway.. Cell reports, (2): [PMID:27425606]
16. Li, Melody M H MM and 11 more authors.. (2017) TRIM25 Enhances the Antiviral Action of Zinc-Finger Antiviral Protein (ZAP).. PLoS pathogens, [PMID:28060952]
17. Zheng, Xiaojiao X and 5 more authors.. (2017) TRIM25 Is Required for the Antiviral Activity of Zinc Finger Antiviral Protein.. Journal of virology, (1): [PMID:28202764]
18. Lian, Huan H and 12 more authors.. (2018) The Zinc-Finger Protein ZCCHC3 Binds RNA and Facilitates Viral RNA Sensing and Activation of the RIG-I-like Receptors.. Immunity, (18): [PMID:30193849]
19. Ban, Junsu J and 5 more authors.. (2018) Human Respiratory Syncytial Virus NS 1 Targets TRIM25 to Suppress RIG-I Ubiquitination and Subsequent RIG-I-Mediated Antiviral Signaling.. Viruses, (14): [PMID:30558248]
20. Chen, Szu-Ting and 15 more authors.. (2019) NLRP12 Regulates Anti-viral RIG-I Activation via Interaction with TRIM25.. Cell host & microbe, (10): [PMID:30902577]

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