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Recombinant Troponin I/TNNI3 Antibody (Ab005500)-Binding ELISA
The binding activity of recombinant Troponin I/TNNI3 antibody with Troponin I/TNNI3 antigen.
Recombinant (23C12); Mouse anti Human Troponin I/TNNI3 Antibody; CLIA, ELISA, LF, GICA, FIA, Detection antibody in LF, GICA, Capture antibody in CLIA, ELISA; Unconjugated
SKU | Size | Availability | Price | Qty |
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Ab005500-10μg | 10μg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $59.90 | |
Ab005500-50μg | 50μg | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $177.90 | |
Ab005500-100μg | 100μg | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $267.90 | |
Ab005500-1mg | 1mg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $447.90 |
Recombinant (23C12); Mouse anti Human Troponin I/TNNI3 Antibody; CLIA, ELISA, LF, GICA, FIA, Detection antibody in LF, GICA, Capture antibody in CLIA, ELISA; Unconjugated
Product Name | Recombinant Troponin I/TNNI3 Antibody - Primary antibody, for Detection antibody in GICA/LF,Capture antibody in FIA various applications, from Human, generated in Mouse, IgG1 |
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Synonyms | Cardiac troponin I; CMD1FF; CMD2A; CMH7MGC116817; CTnI; familial hypertrophic cardiomyopathy 7; RCM1; TNNC1; TNNC1troponin I, cardiac; TNNI3; troponin I type 3 (cardiac); troponin I, cardiac muscle; Troponin I/TNNI3 |
Specifications & Purity | ExactAb™, Validated, Carrier Free, Azide Free, Recombinant, Lot by Lot |
Host Species | Mouse |
Host species | Mouse |
Application | Detection antibody in GICA/LF,Capture antibody in FIA |
Immunogen | E. coli-derived recombinant human CTNI |
Epitope | 24-40 aa |
Conjugation | Unconjugated |
Grade | Azide Free, Carrier Free, ExactAb™, Recombinant, Validated |
Product Description | Recombinant Troponin I/TNNI3 Antibody, 23C12, is a Detection antibody could be used for LF/GICA at 0.4-1.0mg/mL; Capture antibody could be used for CLIA, ELISA at 1.0µg/mL. |
Antibody Type | Primary antibody |
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Clonality | Recombinant |
Isotype | IgG1 |
SDS-PAGE | 150 kDa |
Purification Method | Protein A purified |
Matched Pairs | [Ab005432] Recombinant Troponin I/TNNI3 antibody as Detection antibody in CLIA, ELISA; [Ab005499] Recombinant Troponin I/TNNI3 antibody as Capture antibody in LF/GICA; [Ab005751] Recombinant Troponin I/TNNI3 antibody as Capture antibody in LF/GICA; [Ab00 |
Source | CHO supernatant |
Form | Liquid |
Concentration | Lot by Lot |
Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
Shipped In | Ice chest + Ice pads |
Stability And Storage | Storage: -20℃ for long term storage. Avoid repeated freeze/thaw cycles. |
Recombinant Troponin I/TNNI3 Antibody (Ab005500)-Binding ELISA
The binding activity of recombinant Troponin I/TNNI3 antibody with Troponin I/TNNI3 antigen.
Troponin I/TNNI3 Standard Curve with Antibody Pair (Ab005500 & Ab005432) in CLIA
· Capture antibody: Recombinant Troponin I/TNNI3 Antibody (Ab005500) at 1.0 μg/mL.
· Detection antibody: Recombinant Troponin I/TNNI3 Antibody (Ab005432) at 1.0 μg/mL.
· Linear Range: 0-35000 pg/mL, R ≥ 0.99.
Troponin I/TNNI3 Standard Curve with Antibody Pair (Ab005499 & Ab005748 & Ab005751 & Ab005500) in GICA
· Capture antibody: Recombinant Troponin I/TNNI3 Antibody (Ab005499 & Ab005748 & Ab005751) at 1.0 mg/mL.
· Detection antibody: Recombinant Troponin I/TNNI3 Antibody (Ab005500) at 1.0 mg/mL.
· Linear Range: 0.08-100 ng/mL, LLOD: 0.08 ng/mL.
Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
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Application | Dilution info |
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ELISA |
1 μg/mL |
CLIA |
1 μg/mL
|
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
Lot Number | Certificate Type | Date | Item |
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ZJ23F0500256 | Certificate of Analysis | Jun 02, 2023 | Ab005500 |
ZJ23F0500257 | Certificate of Analysis | Jun 02, 2023 | Ab005500 |
1. Hunkeler, N M NM, Kullman, J J and Murphy, A M AM.. (1991) Troponin I isoform expression in human heart.. Circulation research, [PMID:1934363] |
2. Vallins, W J WJ and 5 more authors.. (1990) Molecular cloning of human cardiac troponin I using polymerase chain reaction.. FEBS letters, (17): [PMID:2226790] |
3. Mittmann, K K, Jaquet, K K and Heilmeyer, L M LM.. (1990) A common motif of two adjacent phosphoserines in bovine, rabbit and human cardiac troponin I.. FEBS letters, (29): [PMID:2226863] |
4. Armour, K L KL, Harris, W J WJ and Tempest, P R PR.. (1993) Cloning and expression in Escherichia coli of the cDNA encoding human cardiac troponin I.. Gene, (15): [PMID:8406024] |
5. Bhavsar, P K PK, Brand, N J NJ, Yacoub, M H MH and Barton, P J PJ.. (1996) Isolation and characterization of the human cardiac troponin I gene (TNNI3).. Genomics, (1): [PMID:8661099] |
6. Kimura, A A and 23 more authors.. (1997) Mutations in the cardiac troponin I gene associated with hypertrophic cardiomyopathy.. Nature genetics, [PMID:9241277] |
7. Keane, N E NE and 5 more authors.. (1997) The ordered phosphorylation of cardiac troponin I by the cAMP-dependent protein kinase--structural consequences and functional implications.. European journal of biochemistry, (1): [PMID:9346285] |
8. Li, M X MX, Spyracopoulos, L L and Sykes, B D BD.. (1999) Binding of cardiac troponin-I147-163 induces a structural opening in human cardiac troponin-C.. Biochemistry, (29): [PMID:10387074] |
9. Niimura, Hideshi H and 6 more authors.. (2002) Sarcomere protein gene mutations in hypertrophic cardiomyopathy of the elderly.. Circulation, (29): [PMID:11815426] |
10. Wang, Xu X, Li, Monica X MX and Sykes, Brian D BD.. (2002) Structure of the regulatory N-domain of human cardiac troponin C in complex with human cardiac troponin I147-163 and bepridil.. The Journal of biological chemistry, (23): [PMID:12060657] |
11. Buscemi, Nina N, Foster, D Brian DB, Neverova, Irina I and Van Eyk, Jennifer E JE.. (2002) p21-activated kinase increases the calcium sensitivity of rat triton-skinned cardiac muscle fiber bundles via a mechanism potentially involving novel phosphorylation of troponin I.. Circulation research, (20): [PMID:12242269] |
12. Mogensen, Jens J and 8 more authors.. (2003) Idiopathic restrictive cardiomyopathy is part of the clinical expression of cardiac troponin I mutations.. The Journal of clinical investigation, [PMID:12531876] |
13. Richard, Pascale P and 16 more authors.. (2003) Hypertrophic cardiomyopathy: distribution of disease genes, spectrum of mutations, and implications for a molecular diagnosis strategy.. Circulation, (6): [PMID:12707239] |
14. Erdmann, J J and 10 more authors.. (2003) Mutation spectrum in a large cohort of unrelated consecutive patients with hypertrophic cardiomyopathy.. Clinical genetics, [PMID:12974739] |
15. Murphy, Ross T RT and 5 more authors.. (2004) Novel mutation in cardiac troponin I in recessive idiopathic dilated cardiomyopathy.. Lancet (London, England), (31): [PMID:15070570] |
16. Haworth, Robert S RS and 6 more authors.. (2004) Protein kinase D is a novel mediator of cardiac troponin I phosphorylation and regulates myofilament function.. Circulation research, (26): [PMID:15514163] |
17. Kedar, Vishram V and 5 more authors.. (2004) Muscle-specific RING finger 1 is a bona fide ubiquitin ligase that degrades cardiac troponin I.. Proceedings of the National Academy of Sciences of the United States of America, (28): [PMID:15601779] |
18. Ingles, J J and 5 more authors.. (2005) Compound and double mutations in patients with hypertrophic cardiomyopathy: implications for genetic testing and counselling.. Journal of medical genetics, [PMID:16199542] |
19. You, Bei B and 7 more authors.. (2009) Phosphorylation of cardiac troponin I by mammalian sterile 20-like kinase 1.. The Biochemical journal, (15): [PMID:18986304] |
20. Carballo, Sebastian S and 9 more authors.. (2009) Identification and functional characterization of cardiac troponin I as a novel disease gene in autosomal dominant dilated cardiomyopathy.. Circulation research, (14): [PMID:19590045] |
21. Millat, Gilles G and 13 more authors.. () Clinical and mutational spectrum in a cohort of 105 unrelated patients with dilated cardiomyopathy.. European journal of medical genetics, [PMID:21846512] |
22. Zhang, Pingbo P and 6 more authors.. (2012) Multiple reaction monitoring to identify site-specific troponin I phosphorylated residues in the failing human heart.. Circulation, (9): [PMID:22972900] |