| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab132947-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $59.90 | |
| Ab132947-50μl | 50μl | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $179.90 | |
| Ab132947-100μl | 100μl | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $299.90 | |
| Ab132947-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,299.90 |
Recombinant; Rabbit anti Human TTF1 Antibody; WB, IHC, ICC, IF; Unconjugated
| Product Name | Recombinant TTF1 Antibody - Primary antibody, specific to TTF1, Rabbit IgG |
|---|---|
| Synonyms | AV026640 antibody | BCH antibody | Benign chorea antibody | BHC antibody | Homeobox protein NK 2 homolog A antibody | Homeobox protein NK-2 homolog A antibody | Homeobox protein Nkx 2.1 antibody | Homeobox protein Nkx-2.1 antibody | Homeobox protein Nkx2. |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 0.4 mg/mL |
| Host species | Rabbit |
| Specificity | TTF1 |
| Immunogen | A synthetic peptide derived from human TTF1 (AA 6-57). |
| Positive Control | WB: HEK293, HeLa, A549, Hep G2 and NIH/3T3 cell lysates. IHC: Human thyoid tissue. ICC/IF: HeLa cells. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description | Rabbit anti Human TTF1 Antibody, Recombinant, could be used for WB, IHC, ICC, IF and so on. |
| Isotype | Rabbit IgG |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Form | Liquid |
| Concentration | 0.4 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant TTF1 Antibody (Ab132947) - Western Blot
All lanes: Recombinant TTF1 Antibody (Ab132947) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 39 kDa
Observed band size: 37 kDa
Exposure time: 2.6 s
Recombinant TTF1 Antibody (Ab132947) - IF
Immunocytochemistry analysis of Thyroid Transcription Factor 1
(green) in HeLa cells using Recombinant TTF1 Antibody (Ab132947) and cells were counterstained with DAPI (blue).
Recombinant TTF1 Antibody (Ab132947) - IHC
Immunohistochemistry analysis of paraffin-embedded Human thyroid using Recombinant TTF1 Antibody (Ab132947). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000"> 1/500-1/1000 |
| IF/ICC |
1/50-1/200
">
1/50-1/200
|
| IHC | 1/50 - 1/100 "> 1/50 - 1/100 |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F0901107 | Certificate of Analysis | Sep 24, 2023 | Ab132947 |
| ZJ23F0901106 | Certificate of Analysis | Sep 24, 2023 | Ab132947 |
| 1. Oguchi, H H, Pan, Y T YT and Kimura, S S.. (1995) The complete nucleotide sequence of the mouse thyroid-specific enhancer-binding protein (T/EBP) gene: extensive identity of the deduced amino acid sequence with the human protein.. Biochimica et biophysica acta, (4): [PMID:7711079] |
| 2. Saiardi, A A, Tassi, V V, De Filippis, V V and Civitareale, D D.. (1995) Cloning and sequence analysis of human thyroid transcription factor 1.. Biochimica et biophysica acta, (4): [PMID:7711080] |
| 3. Ikeda, K K and 5 more authors.. (1995) Gene structure and expression of human thyroid transcription factor-1 in respiratory epithelial cells.. The Journal of biological chemistry, (7): [PMID:7713914] |
| 4. Hamdan, H H and 6 more authors.. (1998) Structure of the human Nkx2.1 gene.. Biochimica et biophysica acta, (13): [PMID:9545595] |
| 5. Pohlenz, Joachim J and 9 more authors.. (2002) Partial deficiency of thyroid transcription factor 1 produces predominantly neurological defects in humans and mice.. The Journal of clinical investigation, [PMID:11854318] |
| 6. Krude, Heiko H and 14 more authors.. (2002) Choreoathetosis, hypothyroidism, and pulmonary alterations due to human NKX2-1 haploinsufficiency.. The Journal of clinical investigation, [PMID:11854319] |
| 7. Breedveld, Guido J GJ and 16 more authors.. (2002) Mutations in TITF-1 are associated with benign hereditary chorea.. Human molecular genetics, (15): [PMID:11971878] |
| 8. Heilig, Roland R and 98 more authors.. (2003) The DNA sequence and analysis of human chromosome 14.. Nature, (6): [PMID:12508121] |
| 9. Doyle, Daniel A DA, Gonzalez, Iris I, Thomas, Becky B and Scavina, Mena M.. (2004) Autosomal dominant transmission of congenital hypothyroidism, neonatal respiratory distress, and ataxia caused by a mutation of NKX2-1.. The Journal of pediatrics, [PMID:15289765] |
| 10. Asmus, F F and 7 more authors.. (2005) A novel TITF-1 mutation causes benign hereditary chorea with response to levodopa.. Neurology, (14): [PMID:15955952] |
| 11. Di Palma, Tina T and 8 more authors.. (2009) TAZ is a coactivator for Pax8 and TTF-1, two transcription factors involved in thyroid differentiation.. Experimental cell research, (15): [PMID:19010321] |
| 12. Ngan, Elly S W ES and 12 more authors.. (2009) A germline mutation (A339V) in thyroid transcription factor-1 (TITF-1/NKX2.1) in patients with multinodular goiter and papillary thyroid carcinoma.. Journal of the National Cancer Institute, (4): [PMID:19176457] |
| 13. Williamson, Scott S, Kirkpatrick, Martin M, Greene, Stephen S and Goudie, David D.. (2014) A novel mutation of NKX2-1 affecting 2 generations with hypothyroidism and choreoathetosis: part of the spectrum of brain-thyroid-lung syndrome.. Journal of child neurology, [PMID:24453141] |
| 14. Thorwarth, Anne A and 28 more authors.. (2014) Comprehensive genotyping and clinical characterisation reveal 27 novel NKX2-1 mutations and expand the phenotypic spectrum.. Journal of medical genetics, [PMID:24714694] |
| 15. Provenzano, Claudia C and 8 more authors.. (2016) Functional characterization of two novel mutations in TTF-1/NKX2.1 homeodomain in patients with benign hereditary chorea.. Journal of the neurological sciences, (15): [PMID:26723978] |
