Recombinant UBE2B Antibody - Primary antibody, specific to UBE2B, Rabbit IgG
Synonyms
E2 17kDa antibody | E2 protein antibody | hHR6B antibody | HR6B antibody | RAD6 homolog B antibody | RAD6B antibody | UBC2 antibody | UBE2B antibody | UBE2B_HUMAN antibody | Ubiquitin carrier protein B antibody | Ubiquitin conjugating enzyme E2 B antibody
Specifications & Purity
ExactAb™, Validated, Recombinant, Lot by Lot
Host species
Rabbit
Specificity
UBE2B
Immunogen
A synthesized peptide derived from human UBE2B (AA 130-152)
Positive Control
WB: Jurkat, K562, Hep G2, NIH/3T3 and C6 cell lysates.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Product Description
Rabbit anti Human UBE2B Antibody, Recombinant, could be used for WB and so on. Application: WB: 1/500-1/2000 Protein Function Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In association with the E3 enzyme BRE1 (RNF20 and/or RNF40), it plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B at 'Lys-120' to form H2BK120ub1. H2BK120ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation. In vitro catalyzes 'Lys-11'-, as well as 'Lys-48'- and 'Lys-63'-linked polyubiquitination. Required for postreplication repair of UV-damaged DNA. Associates to the E3 ligase RAD18 to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. May be involved in neurite outgrowth.
Product Properties
Isotype
Rabbit IgG
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein A purified
Form
Liquid
Concentration
Lot by Lot
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
Recombinant UBE2B Antibody (Ab133222) - Western Blot All lanes: Recombinant UBE2B Antibody (Ab133222) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 17 kDa Observed band size: 20 kDa Exposure time: 62.3 s
Associated Targets(Human)
UBE2BTchemUbiquitin-conjugating enzyme E2 B (0 Activities)