Recombinant UBE2B Antibody - Primary antibody, specific to UBE2B, Rabbit IgG

    Application:
  • WB
Features and benefits
  • Host species: Rabbit
  • Species reactivity(Reacts with): Human,Mouse,Rat
  • Isotype: Rabbit IgG
  • Conjugation: Unconjugated
Item Number
Ab133222
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab133222-10μl
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$69.90
Ab133222-50μl
50μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$199.90
Ab133222-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$289.90
Ab133222-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,199.90

Recombinant; Rabbit anti Human UBE2B Antibody; WB; Unconjugated

Basic Description

Product NameRecombinant UBE2B Antibody - Primary antibody, specific to UBE2B, Rabbit IgG
SynonymsE2 17kDa antibody | E2 protein antibody | hHR6B antibody | HR6B antibody | RAD6 homolog B antibody | RAD6B antibody | UBC2 antibody | UBE2B antibody | UBE2B_HUMAN antibody | Ubiquitin carrier protein B antibody | Ubiquitin conjugating enzyme E2 B antibody
Specifications & PurityExactAb™, Validated, Recombinant, Lot by Lot
Host speciesRabbit
SpecificityUBE2B
ImmunogenA synthesized peptide derived from human UBE2B (AA 130-152)
Positive ControlWB: Jurkat, K562, Hep G2, NIH/3T3 and C6 cell lysates.
ConjugationUnconjugated
GradeExactAb™, Recombinant, Validated
Product Description

Rabbit anti Human UBE2B Antibody, Recombinant, could be used for WB and so on.
Application:
WB: 1/500-1/2000
Protein Function
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In association with the E3 enzyme BRE1 (RNF20 and/or RNF40), it plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B at 'Lys-120' to form H2BK120ub1. H2BK120ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation. In vitro catalyzes 'Lys-11'-, as well as 'Lys-48'- and 'Lys-63'-linked polyubiquitination. Required for postreplication repair of UV-damaged DNA. Associates to the E3 ligase RAD18 to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. May be involved in neurite outgrowth.

Product Properties

IsotypeRabbit IgG
Light Chain Typekappa
SDS-PAGE150 kDa
Purification MethodProtein A purified
FormLiquid
ConcentrationLot by Lot
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Recombinant UBE2B Antibody (Ab133222) - Western Blot
All lanes: Recombinant UBE2B Antibody (Ab133222) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 17 kDa
Observed band size: 20 kDa
Exposure time: 62.3 s

Associated Targets(Human)

UBE2B Tchem Ubiquitin-conjugating enzyme E2 B (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

Mechanisms of Action

Mechanism of ActionAction Typetarget IDTarget NameTarget TypeTarget OrganismBinding Site NameReferences

Application

ApplicationDilution info
WB1/500-1/2000

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1/500-1/2000

Certificates

Certificate of Analysis(COA)

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2 results found

Lot NumberCertificate TypeDateItem
ZJ24F0707668Certificate of AnalysisJul 02, 2024 Ab133222
ZJ24F0707667Certificate of AnalysisJul 02, 2024 Ab133222

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