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Recombinant UBE2D3 Antibody - Primary antibody, specific to UBE2D3, Rabbit IgG

Recombinant

Validated

ExactAb™

0.3 mg/mL

Features and benefits

Short Overview:
Recombinant; Rabbit anti Human UBE2D3 Antibody; WB; Unconjugated
Species reactivity(Reacts with): Human,Mouse,Rat
Isotype: Rabbit IgG

Application:

Item Number

Ab133233

Grouped product items
SKU	Size	Availability	Price
Ab133233-10μl	10μl	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$89.90
Ab133233-50μl	50μl	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$259.90
Ab133233-100μl	100μl	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$379.90
Ab133233-1ml	1ml	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$2,599.90

Recombinant; Rabbit anti Human UBE2D3 Antibody; WB; Unconjugated

View related series

Click to view target information: UBE2D3

Accession#: P61077 Gene ID: 7323 UBE2D3

Basic Description

Product Name	Recombinant UBE2D3 Antibody - Primary antibody, specific to UBE2D3, Rabbit IgG
Synonyms	E2(17)KB3 antibody \| MGC43926 antibody \| MGC5416 antibody \| PRO2116 antibody \| UB2D3_HUMAN antibody \| UBC 4/5 antibody \| UBC4/5 antibody \| UBC4/5 homolog yeast antibody \| UBC4/5, S. cerevisiae, homolog of antibody \| UBCH 5C antibody \| UBCH5C antibody \| Ub
Specifications & Purity	ExactAb™, Validated, Recombinant, 0.3 mg/mL
Host species	Rabbit
Specificity	UBE2D3
Immunogen	A synthetic peptide derived from human UBE2D3 (AA 1-14)
Positive Control	WB: K562, HeLa, HKE293, Jurkat, MCF7, NIH/3T3 and C6 cell lysates.
Conjugation	Unconjugated
Grade	ExactAb™, Recombinant, Validated
Product Description	Rabbit anti Human UBE2D3 Antibody, Recombinant, could be used for WB and so on. Application: WB: 1/500 - 1/1000 Protein Function Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'-, as well as 'Lys-48'-linked polyubiquitination. Cooperates with the E2 CDC34 and the SCF(FBXW11) E3 ligase complex for the polyubiquitination of NFKBIA leading to its subsequent proteasomal degradation. Acts as an initiator E2, priming the phosphorylated NFKBIA target at positions 'Lys-21' and/or 'Lys-22' with a monoubiquitin. Ubiquitin chain elongation is then performed by CDC34, building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. Acts also as an initiator E2, in conjunction with RNF8, for the priming of PCNA. Monoubiquitination of PCNA, and its subsequent polyubiquitination, are essential events in the operation of the DNA damage tolerance (DDT) pathway that is activated after DNA damage caused by UV or chemical agents during S-phase. Associates with the BRCA1/BARD1 E3 ligase complex to perform ubiquitination at DNA damage sites following ionizing radiation leading to DNA repair. Targets DAPK3 for ubiquitination which influences promyelocytic leukemia protein nuclear body (PML-NB) formation in the nucleus. In conjunction with the MDM2 and TOPORS E3 ligases, functions ubiquitination of p53/TP53. Supports NRDP1-mediated ubiquitination and degradation of ERBB3 and of BRUCE which triggers apoptosis. In conjunction with the CBL E3 ligase, targets EGFR for polyubiquitination at the plasma membrane as well as during its internalization and transport on endosomes. In conjunction with the STUB1 E3 quality control E3 ligase, ubiquitinates unfolded proteins to catalyze their immediate destruction.

Product Properties

Isotype	Rabbit IgG
SDS-PAGE	150 kDa
Purification Method	Antigen affinity purified
Form	Liquid
Concentration	0.3 mg/mL
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Recombinant UBE2D3 Antibody (Ab133233) - Western Blot
All lanes: Recombinant UBE2D3 Antibody (Ab133233) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 17 kDa
Observed band size: 17 kDa
Exposure time: 9.6 s

Associated Targets(Human)

UBE2D3 Tchem Ubiquitin-conjugating enzyme E2 D3 (0 Activities)

Discover Target: UBE2D3

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

UBE2D3 Tchem Ubiquitin-conjugating enzyme E2 D3 (121 Activities)

Discover Target: UBE2D3

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Application

Dilution info

1/500-1/1000

Certificates

Certificate of Analysis(COA)

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2 results found

Lot Number	Certificate Type	Date	Item
ZJ24F0607130	Certificate of Analysis	Jun 20, 2024	Ab133233
ZJ24F0607131	Certificate of Analysis	Jun 20, 2024	Ab133233

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