Recombinant; Rabbit anti Human UBC9 Antibody; WB, IHC, ICC, IF; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab133246-10μl | 10μl | In stock | $69.90 | |
| Ab133246-50μl | 50μl | In stock | $209.90 | |
| Ab133246-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $329.90 | |
| Ab133246-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,799.90 |
Recombinant; Rabbit anti Human UBC9 Antibody; WB, IHC, ICC, IF; Unconjugated
| Product Name | Recombinant UBE2I Antibody - Primary antibody, specific to UBC9, Rabbit IgG |
|---|---|
| Synonyms | C358B7.1 antibody | p18 antibody | SUMO 1 protein ligase antibody | SUMO conjugating enzyme UBC9 antibody | SUMO-conjugating enzyme UBC9 antibody | SUMO-protein ligase antibody | SUMO1 protein ligase antibody | UBC9 antibody | UBC9_HUMAN antibody | UBCE9 |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 0.5 mg/mL |
| Host species | Rabbit |
| Specificity | UBC9 |
| Immunogen | A synthetic peptide derived from human UBE2I/UBC9 (AA 28-40) |
| Positive Control | WB: NIH/3T3, A431, MCF7, HeLa, Hep G2, Jurkat and C6 cell lysates. IHC: Human tonsil tissue. ICC/IF: HeLa cells. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description | Rabbit anti Human UBC9 Antibody, Recombinant, could be used for WB, IHC, ICC, IF and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Antigen affinity purified |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant UBE2I Antibody (Ab133246) - Western Blot
All lanes: Recombinant UBE2I Antibody (Ab133246) at 1/4000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 18 kDa
Observed band size: 18 kDa
Exposure time: 7.7 s
Recombinant UBE2I Antibody (Ab133246) - IF/ICC
Immunocytochemistry analysis of SUMO Conjugating Enzyme UBC9 (green) in Hela using Recombinant UBE2I Antibody (Ab133246), and cells were counterstained with DAPI (blue).
Recombinant UBE2I Antibody (Ab133246) - IHC
Immunohistochemistry analysis of paraffin-embedded Human tonsil using Recombinant UBE2I Antibody (Ab133246). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000 |
| ICC/IF |
1/50-1/200
|
| IHC | 1/50 - 1/100 |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0202714 | Certificate of Analysis | Feb 04, 2024 | Ab133246 |
| ZJ24F0202713 | Certificate of Analysis | Feb 04, 2024 | Ab133246 |
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| 29. Tatham, Michael H MH and 5 more authors.. (2005) Unique binding interactions among Ubc9, SUMO and RanBP2 reveal a mechanism for SUMO paralog selection.. Nature structural & molecular biology, [PMID:15608651] |
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| 31. Kim, Young-Eui YE and 5 more authors.. (2005) Requirement of the coiled-coil domain of PML-RARalpha oncoprotein for localization, sumoylation, and inhibition of monocyte differentiation.. Biochemical and biophysical research communications, (13): [PMID:15809060] |
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| 37. Capili, Allan D AD and Lima, Christopher D CD.. (2007) Structure and analysis of a complex between SUMO and Ubc9 illustrates features of a conserved E2-Ubl interaction.. Journal of molecular biology, (8): [PMID:17466333] |
| 38. Cheng, Z Z and 21 more authors.. (2008) Functional characterization of TIP60 sumoylation in UV-irradiated DNA damage response.. Oncogene, (7): [PMID:17704809] |
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| 57. Yamashita, Daisuke D, Moriuchi, Takanobu T, Osumi, Takashi T and Hirose, Fumiko F.. (2016) Transcription Factor hDREF Is a Novel SUMO E3 Ligase of Mi2α.. The Journal of biological chemistry, (27): [PMID:27068747] |
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