| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab133246-10μl | 10μl | In stock | $69.90 | |
| Ab133246-50μl | 50μl | In stock | $209.90 | |
| Ab133246-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $329.90 | |
| Ab133246-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,799.90 |
Recombinant; Rabbit anti Human UBC9 Antibody; WB, IHC, ICC, IF; Unconjugated
| Product Name | Recombinant UBE2I Antibody - Primary antibody, specific to UBC9, Rabbit IgG |
|---|---|
| Synonyms | C358B7.1 antibody | p18 antibody | SUMO 1 protein ligase antibody | SUMO conjugating enzyme UBC9 antibody | SUMO-conjugating enzyme UBC9 antibody | SUMO-protein ligase antibody | SUMO1 protein ligase antibody | UBC9 antibody | UBC9_HUMAN antibody | UBCE9 |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 0.5 mg/mL |
| Host species | Rabbit |
| Specificity | UBC9 |
| Immunogen | A synthetic peptide derived from human UBE2I/UBC9 (AA 28-40) |
| Positive Control | WB: NIH/3T3, A431, MCF7, HeLa, Hep G2, Jurkat and C6 cell lysates. IHC: Human tonsil tissue. ICC/IF: HeLa cells. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description | Rabbit anti Human UBC9 Antibody, Recombinant, could be used for WB, IHC, ICC, IF and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Antigen affinity purified |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant UBE2I Antibody (Ab133246) - Western Blot
All lanes: Recombinant UBE2I Antibody (Ab133246) at 1/4000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 18 kDa
Observed band size: 18 kDa
Exposure time: 7.7 s
Recombinant UBE2I Antibody (Ab133246) - IF/ICC
Immunocytochemistry analysis of SUMO Conjugating Enzyme UBC9 (green) in Hela using Recombinant UBE2I Antibody (Ab133246), and cells were counterstained with DAPI (blue).
Recombinant UBE2I Antibody (Ab133246) - IHC
Immunohistochemistry analysis of paraffin-embedded Human tonsil using Recombinant UBE2I Antibody (Ab133246). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000"> 1/500-1/1000 |
| IF/ICC |
1/50-1/200
">
1/50-1/200
|
| IHC | 1/50 - 1/100 "> 1/50 - 1/100 |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0202714 | Certificate of Analysis | Feb 04, 2024 | Ab133246 |
| ZJ24F0202713 | Certificate of Analysis | Feb 04, 2024 | Ab133246 |
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| 2. Tatham MH, Jaffray E, Vaughan OA, Desterro JM, Botting CH, Naismith JH, Hay RT. (2001) Polymeric chains of SUMO-2 and SUMO-3 are conjugated to protein substrates by SAE1/SAE2 and Ubc9.. J Biol Chem, 276 (38): (35368-74). [PMID:11451954] [10.1021/op500134e] |
| 3. Francis Impens,Lilliana Radoshevich,Pascale Cossart,David Ribet. (2014-08-13) Mapping of SUMO sites and analysis of SUMOylation changes induced by external stimuli.. Proceedings of the National Academy of Sciences of the United States of America, 111 ((34)): (12432-12437). [PMID:25114211] |
| 4. Aileen Y Alontaga,Nigus D Ambaye,Yi-Jia Li,Ramir Vega,Chih-Hong Chen,Krzysztof P Bzymek,John C Williams,Weidong Hu,Yuan Chen. (2015-04-29) RWD Domain as an E2 (Ubc9)-Interaction Module.. The Journal of biological chemistry, 290 ((27)): (16550-16559). [PMID:25918163] |
| 5. Sharon Gauci,Andreas O Helbig,Monique Slijper,Jeroen Krijgsveld,Albert J R Heck,Shabaz Mohammed. (2009-05-06) Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.. Analytical chemistry, 81 ((11)): (4493-4501). [PMID:19413330] |
| 6. Fang-Ting Kuo,Ikuko K Bentsi-Barnes,Gillian M Barlow,Jeehyeon Bae,Margareta D Pisarska. (2009-09-12) Sumoylation of forkhead L2 by Ubc9 is required for its activity as a transcriptional repressor of the Steroidogenic Acute Regulatory gene.. Cellular signalling, 21 ((12)): (1935-1944). [PMID:19744555] |
| 7. Kim YS, Keyser SG, Schneekloth JS.. (2014) Synthesis of 2',3',4'-trihydroxyflavone (2-D08), an inhibitor of protein sumoylation.. Bioorg Med Chem Lett, 24 (4): (1094-1097). [PMID:24468414] [10.1016/j.bmcl.2014.01.010] |
| 8. Leyva MJ, Kim YS, Peach ML, Schneekloth JS.. (2015) Synthetic derivatives of the SUMO consensus sequence provide a basis for improved substrate recognition.. Bioorg Med Chem Lett, 25 (10): (2146-2151). [PMID:25881829] [10.1016/j.bmcl.2015.03.069] |
| 9. Ni S, Chen X, Yu Q, Xu Y, Hu Z, Zhang J, Zhang W, Li B, Yang X, Mao F, Huang J, Sun Y, Li J, Jia L.. (2020) Discovery of candesartan cilexetic as a novel neddylation inhibitor for suppressing tumor growth.. Eur J Med Chem, 185 (111848-111848). [PMID:31732254] [10.1016/j.ejmech.2019.111848] |
| 10. Watanabe, T K TK and 14 more authors.. (1996) Cloning, expression, and mapping of UBE2I, a novel gene encoding a human homologue of yeast ubiquitin-conjugating enzymes which are critical for regulating the cell cycle.. Cytogenetics and cell genetics, [PMID:8565643] |
| 11. Kovalenko, O V OV and 7 more authors.. (1996) Mammalian ubiquitin-conjugating enzyme Ubc9 interacts with Rad51 recombination protein and localizes in synaptonemal complexes.. Proceedings of the National Academy of Sciences of the United States of America, (2): [PMID:8610150] |
| 12. Jiang, W W and Koltin, Y Y.. (1996) Two-hybrid interaction of a human UBC9 homolog with centromere proteins of Saccharomyces cerevisiae.. Molecular & general genetics : MGG, (23): [PMID:8668125] |
| 13. Yasugi, T T and Howley, P M PM.. (1996) Identification of the structural and functional human homolog of the yeast ubiquitin conjugating enzyme UBC9.. Nucleic acids research, (1): [PMID:8668529] |
| 14. Wang, Z Y ZY and 9 more authors.. (1996) Molecular cloning of the cDNA and chromosome localization of the gene for human ubiquitin-conjugating enzyme 9.. The Journal of biological chemistry, (4): [PMID:8798754] |
| 15. Hateboer, G G and 5 more authors.. (1996) mUBC9, a novel adenovirus E1A-interacting protein that complements a yeast cell cycle defect.. The Journal of biological chemistry, (18): [PMID:8824223] |
| 16. Tachibana, M M and 5 more authors.. (1996) Assignment of the gene for a ubiquitin-conjugating enzyme (UBE2I) to human chromosome band 16p13.3 by in situ hybridization.. Cytogenetics and cell genetics, [PMID:9067428] |
| 17. Masson, M M, Menissier-de Murcia, J J, Mattei, M G MG, de Murcia, G G and Niedergang, C P CP.. (1997) Poly(ADP-ribose) polymerase interacts with a novel human ubiquitin conjugating enzyme: hUbc9.. Gene, (6): [PMID:9197546] |
| 18. Tong, H H, Hateboer, G G, Perrakis, A A, Bernards, R R and Sixma, T K TK.. (1997) Crystal structure of murine/human Ubc9 provides insight into the variability of the ubiquitin-conjugating system.. The Journal of biological chemistry, (22): [PMID:9261152] |
| 19. Hahn, S L SL, Wasylyk, B B, Criqui-Filipe, P P and Criqui, P P.. (1997) Modulation of ETS-1 transcriptional activity by huUBC9, a ubiquitin-conjugating enzyme.. Oncogene, (18): [PMID:9333025] |
| 20. Hu, G G and 5 more authors.. (1997) Mammalian homologs of seven in absentia regulate DCC via the ubiquitin-proteasome pathway.. Genes & development, (15): [PMID:9334332] |
| 21. Poukka, H H, Aarnisalo, P P, Karvonen, U U, Palvimo, J J JJ and Jänne, O A OA.. (1999) Ubc9 interacts with the androgen receptor and activates receptor-dependent transcription.. The Journal of biological chemistry, (2): [PMID:10383460] |
| 22. Shi, Y Y, Zou, M M, Farid, N R NR and Paterson, M C MC.. (2000) Association of FHIT (fragile histidine triad), a candidate tumour suppressor gene, with the ubiquitin-conjugating enzyme hUBC9.. The Biochemical journal, (1): [PMID:11085938] |
| 23. Daniels, R J RJ and 10 more authors.. (2001) Sequence, structure and pathology of the fully annotated terminal 2 Mb of the short arm of human chromosome 16.. Human molecular genetics, (15): [PMID:11157797] |
| 24. Bernier-Villamor, Victor V, Sampson, Deborah A DA, Matunis, Michael J MJ and Lima, Christopher D CD.. (2002) Structural basis for E2-mediated SUMO conjugation revealed by a complex between ubiquitin-conjugating enzyme Ubc9 and RanGAP1.. Cell, (8): [PMID:11853669] |
| 25. Eloranta, Jyrki J JJ and Hurst, Helen C HC.. (2002) Transcription factor AP-2 interacts with the SUMO-conjugating enzyme UBC9 and is sumolated in vivo.. The Journal of biological chemistry, (23): [PMID:12072434] |
| 26. Tatham, Michael H MH, Chen, Yuan Y and Hay, Ronald T RT.. (2003) Role of two residues proximal to the active site of Ubc9 in substrate recognition by the Ubc9.SUMO-1 thiolester complex.. Biochemistry, (25): [PMID:12641448] |
| 27. Tatham, Michael H MH and 8 more authors.. (2003) Role of an N-terminal site of Ubc9 in SUMO-1, -2, and -3 binding and conjugation.. Biochemistry, (26): [PMID:12924945] |
| 28. Pichler, Andrea A, Knipscheer, Puck P, Saitoh, Hisato H, Sixma, Titia K TK and Melchior, Frauke F.. (2004) The RanBP2 SUMO E3 ligase is neither HECT- nor RING-type.. Nature structural & molecular biology, [PMID:15378033] |
| 29. Tatham, Michael H MH and 5 more authors.. (2005) Unique binding interactions among Ubc9, SUMO and RanBP2 reveal a mechanism for SUMO paralog selection.. Nature structural & molecular biology, [PMID:15608651] |
| 30. Martin, Joel J and 121 more authors.. (2004) The sequence and analysis of duplication-rich human chromosome 16.. Nature, (23): [PMID:15616553] |
| 31. Kim, Young-Eui YE and 5 more authors.. (2005) Requirement of the coiled-coil domain of PML-RARalpha oncoprotein for localization, sumoylation, and inhibition of monocyte differentiation.. Biochemical and biophysical research communications, (13): [PMID:15809060] |
| 32. Reverter, David D and Lima, Christopher D CD.. (2005) Insights into E3 ligase activity revealed by a SUMO-RanGAP1-Ubc9-Nup358 complex.. Nature, (2): [PMID:15931224] |
| 33. Li, Tianwei T and 6 more authors.. (2006) A general approach for investigating enzymatic pathways and substrates for ubiquitin-like modifiers.. Archives of biochemistry and biophysics, (1): [PMID:16620772] |
| 34. Pan, Xin X and 12 more authors.. (2006) Ubc9 interacts with SOX4 and represses its transcriptional activity.. Biochemical and biophysical research communications, (9): [PMID:16631117] |
| 35. Yunus, Ali A AA and Lima, Christopher D CD.. (2006) Lysine activation and functional analysis of E2-mediated conjugation in the SUMO pathway.. Nature structural & molecular biology, [PMID:16732283] |
| 36. Tomoiu, Andru A, Gravel, Annie A, Tanguay, Robert M RM and Flamand, Louis L.. (2006) Functional interaction between human herpesvirus 6 immediate-early 2 protein and ubiquitin-conjugating enzyme 9 in the absence of sumoylation.. Journal of virology, [PMID:17005699] |
| 37. Capili, Allan D AD and Lima, Christopher D CD.. (2007) Structure and analysis of a complex between SUMO and Ubc9 illustrates features of a conserved E2-Ubl interaction.. Journal of molecular biology, (8): [PMID:17466333] |
| 38. Cheng, Z Z and 21 more authors.. (2008) Functional characterization of TIP60 sumoylation in UV-irradiated DNA damage response.. Oncogene, (7): [PMID:17704809] |
| 39. Carbia-Nagashima, Alberto A and 7 more authors.. (2007) RSUME, a small RWD-containing protein, enhances SUMO conjugation and stabilizes HIF-1alpha during hypoxia.. Cell, (19): [PMID:17956732] |
| 40. Lee, Bongyong B and Muller, Mark T MT.. (2009) SUMOylation enhances DNA methyltransferase 1 activity.. The Biochemical journal, (15): [PMID:19450230] |
| 41. Chang, Tsung-Hsien and 6 more authors.. (2009) Ebola Zaire virus blocks type I interferon production by exploiting the host SUMO modification machinery.. PLoS pathogens, [PMID:19557165] |
| 42. Figueroa-Romero, Claudia C and 8 more authors.. (2009) SUMOylation of the mitochondrial fission protein Drp1 occurs at multiple nonconsensus sites within the B domain and is linked to its activity cycle.. FASEB journal : official publication of the Federation of American Societies for Experimental Biology, [PMID:19638400] |
| 43. Yousef, A F AF and 8 more authors.. (2010) Identification of a molecular recognition feature in the E1A oncoprotein that binds the SUMO conjugase UBC9 and likely interferes with polySUMOylation.. Oncogene, (19): [PMID:20543865] |
| 44. Grünwald, Marlene M and Bono, Fulvia F.. (2011) Structure of Importin13-Ubc9 complex: nuclear import and release of a key regulator of sumoylation.. The EMBO journal, (19): [PMID:21139563] |
| 45. Bentz, Gretchen L GL, Whitehurst, Christopher B CB and Pagano, Joseph S JS.. (2011) Epstein-Barr virus latent membrane protein 1 (LMP1) C-terminal-activating region 3 contributes to LMP1-mediated cellular migration via its interaction with Ubc9.. Journal of virology, [PMID:21795333] |
| 46. Cong, Lin L, Pakala, Suresh B SB, Ohshiro, Kazufumi K, Li, Da-Qiang DQ and Kumar, Rakesh R.. (2011) SUMOylation and SUMO-interacting motif (SIM) of metastasis tumor antigen 1 (MTA1) synergistically regulate its transcriptional repressor function.. The Journal of biological chemistry, (23): [PMID:21965678] |
| 47. Bennett, Richard L RL, Pan, Yu Y, Christian, Jaime J, Hui, Teng T and May, W Stratford WS.. (2012) The RAX/PACT-PKR stress response pathway promotes p53 sumoylation and activation, leading to G₁ arrest.. Cell cycle (Georgetown, Tex.), (15): [PMID:22214662] |
| 48. Su, Yee-Fun; Yang, Tsunghan; Huang, Hoting; Liu, Leroy F and Hwang, Jaulang.. (2012) Phosphorylation of Ubc9 by Cdk1 enhances SUMOylation activity.. PloS one, [PMID:22509284] |
| 49. Van Damme, Petra P and 15 more authors.. (2012) N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB.. Proceedings of the National Academy of Sciences of the United States of America, (31): [PMID:22814378] |
| 50. Oh, Yohan Y and Chung, Kwang Chul KC.. (2013) UHRF2, a ubiquitin E3 ligase, acts as a small ubiquitin-like modifier E3 ligase for zinc finger protein 131.. The Journal of biological chemistry, (29): [PMID:23404503] |
| 51. Gerez, Juan J and 8 more authors.. (2013) In silico structural and functional characterization of the RSUME splice variants.. PloS one, [PMID:23469069] |
| 52. Druker, Jimena J and 8 more authors.. (2013) RSUME enhances glucocorticoid receptor SUMOylation and transcriptional activity.. Molecular and cellular biology, [PMID:23508108] |
| 53. Richard, Patricia P, Feng, Shuang S and Manley, James L JL.. (2013) A SUMO-dependent interaction between Senataxin and the exosome, disrupted in the neurodegenerative disease AOA2, targets the exosome to sites of transcription-induced DNA damage.. Genes & development, (15): [PMID:24105744] |
| 54. Hendriks, Ivo A; Treffers, Louise W; Verlaan-de Vries, Matty; Olsen, Jesper V and Vertegaal, Alfred C O.. (2015) SUMO-2 Orchestrates Chromatin Modifiers in Response to DNA Damage.. Cell reports, (17): [PMID:25772364] |
| 55. Cappadocia, Laurent L, Pichler, Andrea A and Lima, Christopher D CD.. (2015) Structural basis for catalytic activation by the human ZNF451 SUMO E3 ligase.. Nature structural & molecular biology, [PMID:26524494] |
| 56. Sin, Yooksil Y, Tanaka, Kiyoji K and Saijo, Masafumi M.. (2016) The C-terminal Region and SUMOylation of Cockayne Syndrome Group B Protein Play Critical Roles in Transcription-coupled Nucleotide Excision Repair.. The Journal of biological chemistry, (15): [PMID:26620705] |
| 57. Yamashita, Daisuke D, Moriuchi, Takanobu T, Osumi, Takashi T and Hirose, Fumiko F.. (2016) Transcription Factor hDREF Is a Novel SUMO E3 Ligase of Mi2α.. The Journal of biological chemistry, (27): [PMID:27068747] |
| 58. Liang, Ya-Chen YC and 5 more authors.. (2016) SUMO5, a Novel Poly-SUMO Isoform, Regulates PML Nuclear Bodies.. Scientific reports, (23): [PMID:27211601] |
