| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab133260-10μl | 10μl | In stock | $69.90 | |
| Ab133260-50μl | 50μl | In stock | $199.90 | |
| Ab133260-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $299.90 | |
| Ab133260-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,499.90 |
Recombinant; Rabbit anti Human Ube2N / Ubc13 Antibody; WB; Unconjugated
| Product Name | Recombinant Ube2N/Ubc13 Antibody - Primary antibody, specific to UBE2N, Rabbit IgG |
|---|---|
| Synonyms | Bendless-like ubiquitin-conjugating enzyme | E2 ubiquitin-conjugating enzyme N | EC 2.3.2.23 | Ubc13 | UbcH13 | Ubiquitin carrier protein N | Ubiquitin-protein ligase N | Bendless like ubiquitin conjugating enzyme antibody | Bendless-like ubiquitin-conjug |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 0.7 mg/mL |
| Host species | Rabbit |
| Specificity | UBE2N |
| Immunogen | A synthetic peptide derived from human Ube2N/Ubc13 (AA 1-50) |
| Positive Control | WB: Jurkat, Raji, HEK293, U2OS, NIH/3T3 and C6 cell lysates. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description |
Rabbit anti Human Ube2N / Ubc13 Antibody, Recombinant, could be used for WB and so on. |
| Isotype | Rabbit IgG |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Form | Liquid |
| Concentration | 0.7 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant Ube2N/Ubc13 Antibody (Ab133260) - Western Blot
All lanes: Recombinant Ube2N/Ubc13 Antibody (Ab133260) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 17 kDa
Observed band size: 16 kDa
Exposure time: 63.9 s
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000"> 1/500-1/1000 |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0505357 | Certificate of Analysis | May 10, 2024 | Ab133260 |
| ZJ24F0505356 | Certificate of Analysis | May 10, 2024 | Ab133260 |
| 1. Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M. (2009) Lysine acetylation targets protein complexes and co-regulates major cellular functions.. Science, 325 (5942): (834-40). [PMID:19608861] [10.1021/op500134e] |
| 2. Chen H, Wu G, Gao S, Guo R, Zhao Z, Yuan H, Liu S, Wu J, Lu X, Yuan X, Yu Z, Zu X, Xie N, Yang N, Hu Z, Sun Q, Zhang W.. (2017) Discovery of Potent Small-Molecule Inhibitors of Ubiquitin-Conjugating Enzyme UbcH5c from α-Santonin Derivatives.. J Med Chem, 60 (16): (6828-6852). [PMID:28696694] [10.1021/acs.jmedchem.6b01829] |
| 3. Yamaguchi, T T and 6 more authors.. (1996) Cloning and expression of cDNA encoding a human ubiquitin-conjugating enzyme similar to the Drosophila bendless gene product.. Journal of biochemistry, [PMID:8902611] |
| 4. Hofmann, R M RM and Pickart, C M CM.. (1999) Noncanonical MMS2-encoded ubiquitin-conjugating enzyme functions in assembly of novel polyubiquitin chains for DNA repair.. Cell, (5): [PMID:10089880] |
| 5. Moraes, T F TF and 6 more authors.. (2001) Crystal structure of the human ubiquitin conjugating enzyme complex, hMms2-hUbc13.. Nature structural biology, [PMID:11473255] |
| 6. Bothos, John J, Summers, Matthew K MK, Venere, Monica M, Scolnick, Daniel M DM and Halazonetis, Thanos D TD.. (2003) The Chfr mitotic checkpoint protein functions with Ubc13-Mms2 to form Lys63-linked polyubiquitin chains.. Oncogene, (16): [PMID:14562038] |
| 7. Takeuchi, Tomoharu T and Yokosawa, Hideyoshi H.. (2005) ISG15 modification of Ubc13 suppresses its ubiquitin-conjugating activity.. Biochemical and biophysical research communications, (14): [PMID:16112642] |
| 8. Zou, Weiguo W and 6 more authors.. (2005) ISG15 modification of ubiquitin E2 Ubc13 disrupts its ability to form thioester bond with ubiquitin.. Biochemical and biophysical research communications, (14): [PMID:16122702] |
| 9. Plans, Vanessa V and 5 more authors.. (2006) The RING finger protein RNF8 recruits UBC13 for lysine 63-based self polyubiquitylation.. Journal of cellular biochemistry, (15): [PMID:16215985] |
| 10. Zhang, Minghao M and 6 more authors.. (2005) Chaperoned ubiquitylation--crystal structures of the CHIP U box E3 ubiquitin ligase and a CHIP-Ubc13-Uev1a complex.. Molecular cell, (23): [PMID:16307917] |
| 11. Unk, Ildiko I and 9 more authors.. (2006) Human SHPRH is a ubiquitin ligase for Mms2-Ubc13-dependent polyubiquitylation of proliferating cell nuclear antigen.. Proceedings of the National Academy of Sciences of the United States of America, (28): [PMID:17108083] |
| 12. Motegi, Akira A and 5 more authors.. (2006) Human SHPRH suppresses genomic instability through proliferating cell nuclear antigen polyubiquitination.. The Journal of cell biology, (4): [PMID:17130289] |
| 13. Lamothe, Betty B and 5 more authors.. (2007) Site-specific Lys-63-linked tumor necrosis factor receptor-associated factor 6 auto-ubiquitination is a critical determinant of I kappa B kinase activation.. The Journal of biological chemistry, (9): [PMID:17135271] |
| 14. Unk, Ildiko I and 7 more authors.. (2008) Human HLTF functions as a ubiquitin ligase for proliferating cell nuclear antigen polyubiquitination.. Proceedings of the National Academy of Sciences of the United States of America, (11): [PMID:18316726] |
| 15. Scheper, Johanna J, Oliva, Baldo B, Villà-Freixa, Jordi J and Thomson, Timothy M TM.. (2009) Analysis of electrostatic contributions to the selectivity of interactions between RING-finger domains and ubiquitin-conjugating enzymes.. Proteins, [PMID:18615712] |
| 16. Motegi, Akira A and 10 more authors.. (2008) Polyubiquitination of proliferating cell nuclear antigen by HLTF and SHPRH prevents genomic instability from stalled replication forks.. Proceedings of the National Academy of Sciences of the United States of America, (26): [PMID:18719106] |
| 17. Stewart, Grant S GS and 19 more authors.. (2009) The RIDDLE syndrome protein mediates a ubiquitin-dependent signaling cascade at sites of DNA damage.. Cell, (6): [PMID:19203578] |
| 18. Tcherpakov, Marianna M and 5 more authors.. (2009) Regulation of endoplasmic reticulum-associated degradation by RNF5-dependent ubiquitination of JNK-associated membrane protein (JAMP).. The Journal of biological chemistry, (1): [PMID:19269966] |
| 19. Marteijn, J A JA and 11 more authors.. (2009) The ubiquitin ligase Triad1 inhibits myelopoiesis through UbcH7 and Ubc13 interacting domains.. Leukemia, [PMID:19340006] |
| 20. Liu, Caini C and 9 more authors.. (2009) Act1, a U-box E3 ubiquitin ligase for IL-17 signaling.. Science signaling, (13): [PMID:19825828] |
| 21. David, Yael Y, Ziv, Tamar T, Admon, Arie A and Navon, Ami A.. (2010) The E2 ubiquitin-conjugating enzymes direct polyubiquitination to preferred lysines.. The Journal of biological chemistry, (19): [PMID:20061386] |
| 22. Nakada, Shinichiro S and 13 more authors.. (2010) Non-canonical inhibition of DNA damage-dependent ubiquitination by OTUB1.. Nature, (19): [PMID:20725033] |
| 23. Pertel, Thomas T and 16 more authors.. (2011) TRIM5 is an innate immune sensor for the retrovirus capsid lattice.. Nature, (21): [PMID:21512573] |
| 24. Cotta-Ramusino, Cecilia C and 5 more authors.. (2011) A DNA damage response screen identifies RHINO, a 9-1-1 and TopBP1 interacting protein required for ATR signaling.. Science (New York, N.Y.), (10): [PMID:21659603] |
| 25. Juang, Yu-Chi YC and 15 more authors.. (2012) OTUB1 co-opts Lys48-linked ubiquitin recognition to suppress E2 enzyme function.. Molecular cell, (10): [PMID:22325355] |
| 26. Wiener, Reuven R, Zhang, Xiangbin X, Wang, Tao T and Wolberger, Cynthia C.. (2012) The mechanism of OTUB1-mediated inhibition of ubiquitination.. Nature, (22): [PMID:22367539] |
| 27. Campbell, Stephen J SJ and 6 more authors.. (2012) Molecular insights into the function of RING finger (RNF)-containing proteins hRNF8 and hRNF168 in Ubc13/Mms2-dependent ubiquitylation.. The Journal of biological chemistry, (6): [PMID:22589545] |
| 28. Bade, Veronika N; Nickels, Jochen; Keusekotten, Kirstin and Praefcke, Gerrit J K.. (2012) Covalent protein modification with ISG15 via a conserved cysteine in the hinge region.. PloS one, [PMID:22693631] |
| 29. Shi, Yuheng Y and 7 more authors.. (2017) Ube2D3 and Ube2N are essential for RIG-I-mediated MAVS aggregation in antiviral innate immunity.. Nature communications, (4): [PMID:28469175] |
