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Size

Availability

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Ab133260-10μl

10μl

In stock

$69.90

Ab133260-50μl

50μl

In stock

$199.90

Ab133260-100μl

100μl

Available within 8-12 weeks(?)

$299.90

Ab133260-1ml

1ml

Available within 8-12 weeks(?)

$2,499.90

Basic Description

Product Name	Recombinant Ube2N/Ubc13 Antibody - Primary antibody, specific to UBE2N, Rabbit IgG
Synonyms	Bendless-like ubiquitin-conjugating enzyme \| E2 ubiquitin-conjugating enzyme N \| EC 2.3.2.23 \| Ubc13 \| UbcH13 \| Ubiquitin carrier protein N \| Ubiquitin-protein ligase N \| Bendless like ubiquitin conjugating enzyme antibody \| Bendless-like ubiquitin-conjug
Specifications & Purity	ExactAb™, Validated, Recombinant, 0.7 mg/mL
Host species	Rabbit
Specificity	UBE2N
Immunogen	A synthetic peptide derived from human Ube2N/Ubc13 (AA 1-50)
Positive Control	WB: Jurkat, Raji, HEK293, U2OS, NIH/3T3 and C6 cell lysates.
Conjugation	Unconjugated
Grade	ExactAb™, Recombinant, Validated
Product Description	Rabbit anti Human Ube2N / Ubc13 Antibody, Recombinant, could be used for WB and so on. Application · WB: 1/500-1/1000 Protein Function The UBE2V1-UBE2N and UBE2V2-UBE2N heterodimers catalyze the synthesis of non-canonical 'Lys-63'-linked polyubiquitin chains. This type of polyubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. Acts together with the E3 ligases, HLTF and SHPRH, in the 'Lys-63'-linked poly-ubiquitination of PCNA upon genotoxic stress, which is required for DNA repair. Appears to act together with E3 ligase RNF5 in the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD.

Product Properties

Isotype	Rabbit IgG
Light Chain Type	kappa
SDS-PAGE	150 kDa
Purification Method	Protein A purified
Form	Liquid
Concentration	0.7 mg/mL
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Recombinant Ube2N/Ubc13 Antibody (Ab133260) - Western Blot
All lanes: Recombinant Ube2N/Ubc13 Antibody (Ab133260) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 17 kDa
Observed band size: 16 kDa
Exposure time: 63.9 s

Associated Targets(Human)

UBE2N Tchem Ubiquitin-conjugating enzyme E2 N (0 Activities)

Discover Target: UBE2N

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

UBE2N Tchem Ubiquitin-conjugating enzyme E2 N (1570 Activities)

Discover Target: UBE2N

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Application

Application	Dilution info
WB	1/500-1/1000

Certificates

Certificate of Analysis(COA)

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2 results found

Lot Number	Certificate Type	Date	Item
ZJ24F0505357	Certificate of Analysis	May 10, 2024	Ab133260
ZJ24F0505356	Certificate of Analysis	May 10, 2024	Ab133260

FAQs and Articles

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Protocol of Western Blotting For High Molecular Weights

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Western blotting Biological buffer Immunoblotting Protein Electrophoresis High Molecular Weights large molecular proteins WB antibody

References

1. Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M. (2009) Lysine acetylation targets protein complexes and co-regulates major cellular functions.. Science, 325 (5942): (834-40). [PMID:19608861]
2. Chen H, Wu G, Gao S, Guo R, Zhao Z, Yuan H, Liu S, Wu J, Lu X, Yuan X, Yu Z, Zu X, Xie N, Yang N, Hu Z, Sun Q, Zhang W.. (2017) Discovery of Potent Small-Molecule Inhibitors of Ubiquitin-Conjugating Enzyme UbcH5c from α-Santonin Derivatives.. J Med Chem, 60 (16): (6828-6852). [PMID:28696694]
3. Yamaguchi, T T and 6 more authors.. (1996) Cloning and expression of cDNA encoding a human ubiquitin-conjugating enzyme similar to the Drosophila bendless gene product.. Journal of biochemistry, [PMID:8902611]
4. Hofmann, R M RM and Pickart, C M CM.. (1999) Noncanonical MMS2-encoded ubiquitin-conjugating enzyme functions in assembly of novel polyubiquitin chains for DNA repair.. Cell, (5): [PMID:10089880]
5. Moraes, T F TF and 6 more authors.. (2001) Crystal structure of the human ubiquitin conjugating enzyme complex, hMms2-hUbc13.. Nature structural biology, [PMID:11473255]
6. Bothos, John J, Summers, Matthew K MK, Venere, Monica M, Scolnick, Daniel M DM and Halazonetis, Thanos D TD.. (2003) The Chfr mitotic checkpoint protein functions with Ubc13-Mms2 to form Lys63-linked polyubiquitin chains.. Oncogene, (16): [PMID:14562038]
7. Takeuchi, Tomoharu T and Yokosawa, Hideyoshi H.. (2005) ISG15 modification of Ubc13 suppresses its ubiquitin-conjugating activity.. Biochemical and biophysical research communications, (14): [PMID:16112642]
8. Zou, Weiguo W and 6 more authors.. (2005) ISG15 modification of ubiquitin E2 Ubc13 disrupts its ability to form thioester bond with ubiquitin.. Biochemical and biophysical research communications, (14): [PMID:16122702]
9. Plans, Vanessa V and 5 more authors.. (2006) The RING finger protein RNF8 recruits UBC13 for lysine 63-based self polyubiquitylation.. Journal of cellular biochemistry, (15): [PMID:16215985]
10. Zhang, Minghao M and 6 more authors.. (2005) Chaperoned ubiquitylation--crystal structures of the CHIP U box E3 ubiquitin ligase and a CHIP-Ubc13-Uev1a complex.. Molecular cell, (23): [PMID:16307917]
11. Unk, Ildiko I and 9 more authors.. (2006) Human SHPRH is a ubiquitin ligase for Mms2-Ubc13-dependent polyubiquitylation of proliferating cell nuclear antigen.. Proceedings of the National Academy of Sciences of the United States of America, (28): [PMID:17108083]
12. Motegi, Akira A and 5 more authors.. (2006) Human SHPRH suppresses genomic instability through proliferating cell nuclear antigen polyubiquitination.. The Journal of cell biology, (4): [PMID:17130289]
13. Lamothe, Betty B and 5 more authors.. (2007) Site-specific Lys-63-linked tumor necrosis factor receptor-associated factor 6 auto-ubiquitination is a critical determinant of I kappa B kinase activation.. The Journal of biological chemistry, (9): [PMID:17135271]
14. Unk, Ildiko I and 7 more authors.. (2008) Human HLTF functions as a ubiquitin ligase for proliferating cell nuclear antigen polyubiquitination.. Proceedings of the National Academy of Sciences of the United States of America, (11): [PMID:18316726]
15. Scheper, Johanna J, Oliva, Baldo B, Villà-Freixa, Jordi J and Thomson, Timothy M TM.. (2009) Analysis of electrostatic contributions to the selectivity of interactions between RING-finger domains and ubiquitin-conjugating enzymes.. Proteins, [PMID:18615712]
16. Motegi, Akira A and 10 more authors.. (2008) Polyubiquitination of proliferating cell nuclear antigen by HLTF and SHPRH prevents genomic instability from stalled replication forks.. Proceedings of the National Academy of Sciences of the United States of America, (26): [PMID:18719106]
17. Stewart, Grant S GS and 19 more authors.. (2009) The RIDDLE syndrome protein mediates a ubiquitin-dependent signaling cascade at sites of DNA damage.. Cell, (6): [PMID:19203578]
18. Tcherpakov, Marianna M and 5 more authors.. (2009) Regulation of endoplasmic reticulum-associated degradation by RNF5-dependent ubiquitination of JNK-associated membrane protein (JAMP).. The Journal of biological chemistry, (1): [PMID:19269966]
19. Marteijn, J A JA and 11 more authors.. (2009) The ubiquitin ligase Triad1 inhibits myelopoiesis through UbcH7 and Ubc13 interacting domains.. Leukemia, [PMID:19340006]
20. Liu, Caini C and 9 more authors.. (2009) Act1, a U-box E3 ubiquitin ligase for IL-17 signaling.. Science signaling, (13): [PMID:19825828]
21. David, Yael Y, Ziv, Tamar T, Admon, Arie A and Navon, Ami A.. (2010) The E2 ubiquitin-conjugating enzymes direct polyubiquitination to preferred lysines.. The Journal of biological chemistry, (19): [PMID:20061386]
22. Nakada, Shinichiro S and 13 more authors.. (2010) Non-canonical inhibition of DNA damage-dependent ubiquitination by OTUB1.. Nature, (19): [PMID:20725033]
23. Pertel, Thomas T and 16 more authors.. (2011) TRIM5 is an innate immune sensor for the retrovirus capsid lattice.. Nature, (21): [PMID:21512573]
24. Cotta-Ramusino, Cecilia C and 5 more authors.. (2011) A DNA damage response screen identifies RHINO, a 9-1-1 and TopBP1 interacting protein required for ATR signaling.. Science (New York, N.Y.), (10): [PMID:21659603]
25. Juang, Yu-Chi YC and 15 more authors.. (2012) OTUB1 co-opts Lys48-linked ubiquitin recognition to suppress E2 enzyme function.. Molecular cell, (10): [PMID:22325355]
26. Wiener, Reuven R, Zhang, Xiangbin X, Wang, Tao T and Wolberger, Cynthia C.. (2012) The mechanism of OTUB1-mediated inhibition of ubiquitination.. Nature, (22): [PMID:22367539]
27. Campbell, Stephen J SJ and 6 more authors.. (2012) Molecular insights into the function of RING finger (RNF)-containing proteins hRNF8 and hRNF168 in Ubc13/Mms2-dependent ubiquitylation.. The Journal of biological chemistry, (6): [PMID:22589545]
28. Bade, Veronika N; Nickels, Jochen; Keusekotten, Kirstin and Praefcke, Gerrit J K.. (2012) Covalent protein modification with ISG15 via a conserved cysteine in the hinge region.. PloS one, [PMID:22693631]
29. Shi, Yuheng Y and 7 more authors.. (2017) Ube2D3 and Ube2N are essential for RIG-I-mediated MAVS aggregation in antiviral innate immunity.. Nature communications, (4): [PMID:28469175]

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Recombinant Ube2N/Ubc13 Antibody - Primary antibody, specific to UBE2N, Rabbit IgG