A synthetic peptide derived from human VAMP1 (AA 1-45)
Positive Control
WB: Mouse brain, U-2 OS lysates.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Product Description
Rabbit anti Human VAMP1 Antibody, Recombinant, could be used for WB and so on.
Application
· WB: 1/500-1/1000
Protein Function
Involved in the targeting and/or fusion of transport vesicles to their target membrane.
Product Properties
Isotype
Rabbit IgG
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein A purified
Form
Liquid
Concentration
0.42 mg/mL
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
Recombinant VAMP1 Antibody (Ab133851) - Western Blot All lanes: Recombinant VAMP1 Antibody (Ab133851) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 13 kDa Observed band size: 18 kDa Exposure time: 6.7 s
Recombinant VAMP1 Antibody (Ab133851) - Western Blot All lanes: Recombinant VAMP1 Antibody (Ab133851) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 13 kDa Observed band size: 18 kDa Exposure time: 110.8 s
Associated Targets(Human)
VAMP1TbioVesicle-associated membrane protein 1 (0 Activities)
1.Archer, B T BT, Ozçelik, T T, Jahn, R R, Francke, U U and Südhof, T C TC.. (1990) Structures and chromosomal localizations of two human genes encoding synaptobrevins 1 and 2.. The Journal of biological chemistry, (5):[PMID:1976629]
2.Isenmann, S S, Khew-Goodall, Y Y, Gamble, J J, Vadas, M M and Wattenberg, B W BW.. (1998) A splice-isoform of vesicle-associated membrane protein-1 (VAMP-1) contains a mitochondrial targeting signal.. Molecular biology of the cell, [PMID:9658161]
3.Chen, X X, Ji, Z L ZL and Chen, Y Z YZ.. (2002) TTD: Therapeutic Target Database.. Nucleic acids research, (1):[PMID:11752352]
4.Scherer, Steven E SE and 216 more authors.. (2006) The finished DNA sequence of human chromosome 12.. Nature, (16):[PMID:16541075]
5.Yamamoto, Hideyuki H and 9 more authors.. (2012) Specificity of botulinum protease for human VAMP family proteins.. Microbiology and immunology, [PMID:22289120]
6.Bourassa, Cynthia V CV and 14 more authors.. (2012) VAMP1 mutation causes dominant hereditary spastic ataxia in Newfoundland families.. American journal of human genetics, (7):[PMID:22958904]
7.Shen, Xin-Ming XM and 7 more authors.. (2017) Novel synaptobrevin-1 mutation causes fatal congenital myasthenic syndrome.. Annals of clinical and translational neurology, [PMID:28168212]
8.Salpietro, Vincenzo V and 21 more authors.. (2017) Homozygous mutations in VAMP1 cause a presynaptic congenital myasthenic syndrome.. Annals of neurology, [PMID:28253535]
9.Monies, Dorota D and 106 more authors.. (2017) The landscape of genetic diseases in Saudi Arabia based on the first 1000 diagnostic panels and exomes.. Human genetics, [PMID:28600779]
10.Masuyer, Geoffrey G and 7 more authors.. (2018) Structural characterisation of the catalytic domain of botulinum neurotoxin X - high activity and unique substrate specificity.. Scientific reports, (14):[PMID:29540745]
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