A synthetic peptide derived from human VEGFD (AA 242-255)
Positive Control
WB: HeLa, Raji, MCF7, A549, RAW 264.7 and C6 cell lysates. IHC: Human lung cancer tissue. ICC/IF: HeLa cells.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Product Description
Rabbit anti Human VEGFD Antibody, Recombinant, could be used for WB, IHC, ICC, IF and so on. Application: WB: 1/500 - 1/1000 IHC: 1/50-1/100 ICC/IF: 1/50-1/200 Protein Function Post-translational Growth factor active in angiogenesis, lymphangiogenesis and endothelial cell growth, stimulating their proliferation and migration and also has effects on the permeability of blood vessels. May function in the formation of the venous and lymphatic vascular systems during embryogenesis, and also in the maintenance of differentiated lymphatic endothelium in adults. Binds and activates VEGFR-2 (KDR/FLK1) and VEGFR-3 (FLT4) receptors. Activated by phosphorylation at Ser-218 by PDPK1. Autophosphorylated on Ser-377, as part of the activation process. May be phosphorylated at Thr-356 and Ser-360 by MAPK1/ERK2 and MAPK3/ERK1. N-terminal myristoylation results in an activated kinase in the absence of added growth factors. Post-translational Undergoes a complex proteolytic maturation which generates a variety of processed secreted forms with increased activity toward VEGFR-3 and VEGFR-2. VEGF-D first form an antiparallel homodimer linked by disulfide bonds before secretion. The fully processed VEGF-D is composed mostly of two VEGF homology domains (VHDs) bound by non-covalent interactions.
Product Properties
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Antigen affinity purified
Form
Liquid
Concentration
1.5 mg/mL
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
Recombinant VEGFD Antibody (Ab134116) - Western Blot All lanes: Recombinant VEGFD Antibody (Ab134116) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 40 kDa Observed band size: 40, 76 kDa Exposure time: 27.9 s
Recombinant VEGFD Antibody (Ab134116) - IF/ICC Immunocytochemistry analysis of VEGFD (green) in HeLa using Recombinant VEGFD Antibody (Ab134116), and cells were counterstained with DAPI (blue).
Recombinant VEGFD Antibody (Ab134116) - IHC Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Recombinant VEGFD Antibody (Ab134116). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Associated Targets(Human)
VEGFDTbioVascular endothelial growth factor D (0 Activities)
1.Yamada, Y Y, Nezu, J J, Shimane, M M and Hirata, Y Y.. (1997) Molecular cloning of a novel vascular endothelial growth factor, VEGF-D.. Genomics, (15):[PMID:9205122]
2.Achen, M G MG and 7 more authors.. (1998) Vascular endothelial growth factor D (VEGF-D) is a ligand for the tyrosine kinases VEGF receptor 2 (Flk1) and VEGF receptor 3 (Flt4).. Proceedings of the National Academy of Sciences of the United States of America, (20):[PMID:9435229]
3.Rocchigiani, M M and 8 more authors.. (1998) Human FIGF: cloning, gene structure, and mapping to chromosome Xp22.1 between the PIGA and the GRPR genes.. Genomics, (15):[PMID:9479493]
4.Stacker, S A SA and 11 more authors.. (1999) Biosynthesis of vascular endothelial growth factor-D involves proteolytic processing which generates non-covalent homodimers.. The Journal of biological chemistry, (5):[PMID:10542248]
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