A synthetic peptide derived from human Visfatin (AA 330-349)
Positive Control
WB: HeLa, U-2 OS, Raji and C6 cell lysates. IHC: Human lung cancer tissue. ICC/IF: U87-MG cells.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Product Description
Rabbit anti Human Visfatin Antibody, Recombinant, could be used for WB, IHC, ICC, IF and so on.
Application:
WB: 1/500 - 1/1000
IHC: 1/50-1/100
ICC/IF: 1/50-1/200
Protein Function
Catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway.
Product Properties
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Antigen affinity purified
Form
Liquid
Concentration
0.6 mg/mL
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
Recombinant Visfatin Antibody (Ab134271) - Western Blot All lanes: Recombinant Visfatin Antibody (Ab134271) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 56 kDa Observed band size: 54 kDa Exposure time: 10.0 s
Recombinant Visfatin Antibody (Ab134271) - IF/ICC Immunocytochemistry analysis of Nampt (green) in U87-MG using Recombinant Visfatin Antibody (Ab134271) and DAPI (blue).
Recombinant Visfatin Antibody (Ab134271) - IHC Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Recombinant Visfatin Antibody (Ab134271). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.