| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab134682-10μl | 10μl | In stock | $66.90 | |
| Ab134682-50μl | 50μl | In stock | $199.90 | |
| Ab134682-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $339.90 | |
| Ab134682-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,399.90 |
Recombinant; Rabbit anti Human Wilms Tumor Protein Antibody; WB, IHC; Unconjugated
| Product Name | Recombinant Wilms Tumor Protein Antibody - Primary antibody, specific to Wilms Tumor Protein, Rabbit IgG |
|---|---|
| Synonyms | WIT 2 antibody | WT 1 antibody | AWT1 antibody | FWT1 antibody | GUD antibody | NPHS4 antibody | WAGR antibody | Wilms tumor 1 antibody | Wilms Tumor antibody | Wilms tumor protein antibody | Wilms' tumor gene antibody | Wilms' tumor protein antibody | WI |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 0.6 mg/mL |
| Host species | Rabbit |
| Specificity | Wilms Tumor Protein |
| Immunogen | A synthetic peptide derived from human Wilms Tumor Protein (AA 60-250) |
| Positive Control | WB: K562, A549 and HEK293 cell lysates. IHC: Mouse kidney tissue. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description | Rabbit anti Human Wilms Tumor Protein Antibody, Recombinant, could be used for WB, IHC and so on. |
| Isotype | Rabbit IgG |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Form | Liquid |
| Concentration | 0.6 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant Wilms Tumor Protein Antibody (Ab134682) - Western Blot
All lanes: Recombinant Wilms Tumor Protein Antibody (Ab134682) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 49 kDa
Observed band size: 54 kDa
Exposure time: 100.0 s
Recombinant Wilms Tumor Protein Antibody (Ab134682) - Western Blot
All lanes: Recombinant Wilms Tumor Protein Antibody (Ab134682) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 49 kDa
Observed band size: 52, 60 kDa
Exposure time: 52.9 s
Recombinant Wilms Tumor Protein Antibody (Ab134682) - IHC
Immunohistochemistry analysis of paraffin-embedded mouse kidney using Recombinant Wilms Tumor Protein Antibody (Ab134682). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000"> 1/500-1/1000 |
| IHC | 1/50 - 1/100 "> 1/50 - 1/100 |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0202810 | Certificate of Analysis | Feb 21, 2024 | Ab134682 |
| ZJ24F0202809 | Certificate of Analysis | Feb 21, 2024 | Ab134682 |
| 1. Todd D Taylor,Hideki Noguchi,Yasushi Totoki,Atsushi Toyoda,Yoko Kuroki,Ken Dewar,Christine Lloyd,Takehiko Itoh,Tadayuki Takeda,Dae-Won Kim,Xinwei She,Karen F Barlow,Toby Bloom,Elspeth Bruford,Jean L Chang,Christina A Cuomo,Evan Eichler,Michael G FitzGerald,David B Jaffe,Kurt LaButti,Robert Nicol,Hong-Seog Park,Christopher Seaman,Carrie Sougnez,Xiaoping Yang,Andrew R Zimmer,Michael C Zody,Bruce W Birren,Chad Nusbaum,Asao Fujiyama,Masahira Hattori,Jane Rogers,Eric S Lander,Yoshiyuki Sakaki. (2006-03-24) Human chromosome 11 DNA sequence and analysis including novel gene identification.. Nature, 440 ((7083)): (497-500). [PMID:16554811] |
| 2. Bruening, W W and 7 more authors.. (1992) Germline intronic and exonic mutations in the Wilms' tumour gene (WT1) affecting urogenital development.. Nature genetics, [PMID:1302008] |
| 3. Haber, D A DA and Buckler, A J AJ.. (1992) WT1: a novel tumor suppressor gene inactivated in Wilms' tumor.. The New biologist, [PMID:1313285] |
| 4. Little, M H MH and 5 more authors.. (1992) Zinc finger point mutations within the WT1 gene in Wilms tumor patients.. Proceedings of the National Academy of Sciences of the United States of America, (1): [PMID:1317572] |
| 5. Baird, P N PN, Santos, A A, Groves, N N, Jadresic, L L and Cowell, J K JK.. (1992) Constitutional mutations in the WT1 gene in patients with Denys-Drash syndrome.. Human molecular genetics, [PMID:1338906] |
| 6. Gessler, M M, König, A A and Bruns, G A GA.. (1992) The genomic organization and expression of the WT1 gene.. Genomics, [PMID:1572653] |
| 7. Pelletier, J J and 5 more authors.. (1991) WT1 mutations contribute to abnormal genital system development and hereditary Wilms' tumour.. Nature, (3): [PMID:1654525] |
| 8. Pelletier, J J and 9 more authors.. (1991) Germline mutations in the Wilms' tumor suppressor gene are associated with abnormal urogenital development in Denys-Drash syndrome.. Cell, (18): [PMID:1655284] |
| 9. Haber, D A DA and 5 more authors.. (1991) Alternative splicing and genomic structure of the Wilms tumor gene WT1.. Proceedings of the National Academy of Sciences of the United States of America, (1): [PMID:1658787] |
| 10. Buckler, A J AJ, Pelletier, J J, Haber, D A DA, Glaser, T T and Housman, D E DE.. (1991) Isolation, characterization, and expression of the murine Wilms' tumor gene (WT1) during kidney development.. Molecular and cellular biology, [PMID:1671709] |
| 11. Call, K M KM and 9 more authors.. (1990) Isolation and characterization of a zinc finger polypeptide gene at the human chromosome 11 Wilms' tumor locus.. Cell, (9): [PMID:2154335] |
| 12. Gessler, M M and 5 more authors.. (1990) Homozygous deletion in Wilms tumours of a zinc-finger gene identified by chromosome jumping.. Nature, (22): [PMID:2154702] |
| 13. Hamilton, T B TB, Barilla, K C KC and Romaniuk, P J PJ.. (1995) High affinity binding sites for the Wilms' tumour suppressor protein WT1.. Nucleic acids research, (25): [PMID:7862533] |
| 14. Sharma, P M PM, Bowman, M M, Madden, S L SL, Rauscher, F J FJ and Sukumar, S S.. (1994) RNA editing in the Wilms' tumor susceptibility gene, WT1.. Genes & development, (15): [PMID:7926762] |
| 15. Nordenskjöld, A A, Friedman, E E and Anvret, M M.. (1994) WT1 mutations in patients with Denys-Drash syndrome: a novel mutation in exon 8 and paternal allele origin.. Human genetics, [PMID:8112732] |
| 16. Tsuda, M M and 7 more authors.. (1993) Molecular analysis of two Japanese cases of Denys-Drash syndrome.. Journal of inherited metabolic disease, [PMID:8295405] |
| 17. Little, M H MH and 6 more authors.. (1993) Evidence that WT1 mutations in Denys-Drash syndrome patients may act in a dominant-negative fashion.. Human molecular genetics, [PMID:8388765] |
| 18. and Rauscher, F J FJ.. (1993) The WT1 Wilms tumor gene product: a developmentally regulated transcription factor in the kidney that functions as a tumor suppressor.. FASEB journal : official publication of the Federation of American Societies for Experimental Biology, [PMID:8393820] |
| 19. Park, S S and 9 more authors.. (1993) The Wilms tumour gene WT1 is expressed in murine mesoderm-derived tissues and mutated in a human mesothelioma.. Nature genetics, [PMID:8401592] |
| 20. Clarkson, P A PA and 5 more authors.. (1993) Mutational screening of the Wilms's tumour gene, WT1, in males with genital abnormalities.. Journal of medical genetics, [PMID:8411073] |
| 21. Bruening, W W and Pelletier, J J.. (1996) A non-AUG translational initiation event generates novel WT1 isoforms.. The Journal of biological chemistry, (12): [PMID:8621495] |
| 22. Tsuda, M M, Sakiyama, T T, Owada, M M and Chiba, Y Y.. (1996) A newly identified exonic mutation of the WT1 gene in a patient with Denys-Drash syndrome.. Acta paediatrica Japonica : Overseas edition, [PMID:8741319] |
| 23. Ghahremani, M M and 5 more authors.. () A novel mutation H373Y in the Wilms' tumor suppressor gene, WT1, associated with Denys-Drash syndrome.. Human heredity, [PMID:8956030] |
| 24. Schumacher, V V and 8 more authors.. (1997) Correlation of germ-line mutations and two-hit inactivation of the WT1 gene with Wilms tumors of stromal-predominant histology.. Proceedings of the National Academy of Sciences of the United States of America, (15): [PMID:9108089] |
| 25. Kikuchi, H H and 8 more authors.. (1998) Do intronic mutations affecting splicing of WT1 exon 9 cause Frasier syndrome?. Journal of medical genetics, [PMID:9475094] |
| 26. Jeanpierre, C C and 11 more authors.. (1998) Identification of constitutional WT1 mutations, in patients with isolated diffuse mesangial sclerosis, and analysis of genotype/phenotype correlations by use of a computerized mutation database.. American journal of human genetics, [PMID:9529364] |
| 27. Schumacher, V V and 15 more authors.. (1998) Spectrum of early onset nephrotic syndrome associated with WT1 missense mutations.. Kidney international, [PMID:9607189] |
| 28. Kohsaka, T T and 5 more authors.. (1999) Exon 9 mutations in the WT1 gene, without influencing KTS splice isoforms, are also responsible for Frasier syndrome.. Human mutation, [PMID:10571943] |
| 29. Little, M M, Carman, G G and Donaldson, E E.. (2000) Novel WT1 exon 9 mutation (D396Y) in a patient with early onset Denys Drash syndrome.. Human mutation, [PMID:10738002] |
| 30. Ohta, S S, Ozawa, T T, Izumino, K K, Sakuragawa, N N and Fuse, H H.. (2000) A novel missense mutation of the Wt1 gene causing Denys-Drash syndrome with exceptionally mild renal manifestations.. The Journal of urology, [PMID:10799199] |
| 31. Little, N A NA, Hastie, N D ND and Davies, R C RC.. (2000) Identification of WTAP, a novel Wilms' tumour 1-associating protein.. Human molecular genetics, (22): [PMID:11001926] |
| 32. Takata, A A and 5 more authors.. (2000) Constitutional WT1 correlate with clinical features in children with progressive nephropathy.. Journal of medical genetics, [PMID:11182928] |
| 33. Swiatecka-Urban, A A, Mokrzycki, M H MH, Kaskel, F F, Da Silva, F F and Denamur, E E.. (2001) Novel WT1 mutation (C388Y) in a female child with Denys-Drash syndrome.. Pediatric nephrology (Berlin, Germany), [PMID:11519891] |
| 34. Lee, Tae Ho TH, Lwu, Shelly S, Kim, Jungho J and Pelletier, Jerry J.. (2002) Inhibition of Wilms tumor 1 transactivation by bone marrow zinc finger 2, a novel transcriptional repressor.. The Journal of biological chemistry, (22): [PMID:12239212] |
| 35. Matsuzawa-Watanabe, Yumiko Y, Inoue, Jun-Ichiro J and Semba, Kentaro K.. (2003) Transcriptional activity of testis-determining factor SRY is modulated by the Wilms' tumor 1 gene product, WT1.. Oncogene, (11): [PMID:12970737] |
| 36. Royer-Pokora, Brigitte B and 6 more authors.. (2004) Twenty-four new cases of WT1 germline mutations and review of the literature: genotype/phenotype correlations for Wilms tumor development.. American journal of medical genetics. Part A, (15): [PMID:15150775] |
| 37. Ruf, Rainer G RG and 16 more authors.. (2004) Prevalence of WT1 mutations in a large cohort of patients with steroid-resistant and steroid-sensitive nephrotic syndrome.. Kidney international, [PMID:15253707] |
| 38. Wang, Yanping Y and 9 more authors.. (2004) Mutation analysis of five candidate genes in Chinese patients with hypospadias.. European journal of human genetics : EJHG, [PMID:15266301] |
| 39. Hu, Min M and 6 more authors.. (2004) A novel mutation of WT1 exon 9 in a patient with Denys-Drash syndrome and pyloric stenosis.. Pediatric nephrology (Berlin, Germany), [PMID:15349765] |
| 40. Lachenmann, Marcel J MJ and 5 more authors.. (2004) Why zinc fingers prefer zinc: ligand-field symmetry and the hidden thermodynamics of metal ion selectivity.. Biochemistry, (9): [PMID:15518539] |
| 41. Smolen, Gromoslaw A GA, Vassileva, Maria T MT, Wells, Julie J, Matunis, Michael J MJ and Haber, Daniel A DA.. (2004) SUMO-1 modification of the Wilms' tumor suppressor WT1.. Cancer research, (1): [PMID:15520190] |
| 42. Markus, M Andrea MA and 9 more authors.. (2006) WT1 interacts with the splicing protein RBM4 and regulates its ability to modulate alternative splicing in vivo.. Experimental cell research, (15): [PMID:16934801] |
| 43. Yang, L L, Han, Y Y, Suarez Saiz, F F, Saurez Saiz, F F and Minden, M D MD.. (2007) A tumor suppressor and oncogene: the WT1 story.. Leukemia, [PMID:17361230] |
| 44. Stoll, Raphael R and 6 more authors.. (2007) Structure of the Wilms tumor suppressor protein zinc finger domain bound to DNA.. Journal of molecular biology, (5): [PMID:17716689] |
| 45. Suri, Mohnish M and 12 more authors.. (2007) WT1 mutations in Meacham syndrome suggest a coelomic mesothelial origin of the cardiac and diaphragmatic malformations.. American journal of medical genetics. Part A, (1): [PMID:17853480] |
| 46. Weiss, Tristen C TC and Romaniuk, Paul J PJ.. (2009) Contribution of individual amino acids to the RNA binding activity of the Wilms' tumor suppressor protein WT1.. Biochemistry, (13): [PMID:19123921] |
| 47. Rivera, Miguel N MN and 7 more authors.. (2009) The tumor suppressor WTX shuttles to the nucleus and modulates WT1 activity.. Proceedings of the National Academy of Sciences of the United States of America, (19): [PMID:19416806] |
| 48. Büscher, Anja K AK and 13 more authors.. (2010) Immunosuppression and renal outcome in congenital and pediatric steroid-resistant nephrotic syndrome.. Clinical journal of the American Society of Nephrology : CJASN, [PMID:20798252] |
| 49. Hashimoto, Hideharu H and 5 more authors.. (2014) Wilms tumor protein recognizes 5-carboxylcytosine within a specific DNA sequence.. Genes & development, (15): [PMID:25258363] |
