| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab134692-10μl | 10μl | In stock | $66.90 | |
| Ab134692-50μl | 50μl | In stock | $199.90 | |
| Ab134692-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $289.90 | |
| Ab134692-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,599.90 |
Recombinant; Rabbit anti Human WIPI1 Antibody; WB; Unconjugated
| Product Name | Recombinant WIPI1 Antibody - Primary antibody, specific to WIPI1, Rabbit IgG |
|---|---|
| Synonyms | ATG 18 antibody | ATG18 antibody | Atg18 protein homolog antibody | ATG18A antibody | FLJ10055 antibody | WD repeat domain phosphoinositide interacting 1 antibody | WD repeat domain phosphoinositide interacting protein 1 antibody | WD repeat domain phosph |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 1.6 mg/mL |
| Host species | Rabbit |
| Specificity | WIPI1 |
| Immunogen | A synthetic peptide derived from human WIPI1 (AA 55-102) |
| Positive Control | WB: A375, RAW 264.7, NIH/3T3 and C6 cell lysates. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description | Rabbit anti Human WIPI1 Antibody, Recombinant, could be used for WB and so on. |
| Isotype | Rabbit IgG |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Form | Liquid |
| Concentration | 1.6 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant WIPI1 Antibody (Ab134692) - Western Blot
All lanes: Recombinant WIPI1 Antibody (Ab134692) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 49 kDa
Observed band size: 48 kDa
Exposure time: 27.8 s
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000"> 1/500-1/1000 |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Chart | Certificate Type | Date | Item |
|---|---|---|---|---|
 ZJ24F0405059 | Certificate of Analysis | Apr 30, 2024 | Ab134692 | |
| ZJ24F0405058 | Certificate of Analysis | Apr 30, 2024 | Ab134692 |
| 1. Jeffries, Tim R TR, Dove, Stephen K SK, Michell, Robert H RH and Parker, Peter J PJ.. (2004) PtdIns-specific MPR pathway association of a novel WD40 repeat protein, WIPI49.. Molecular biology of the cell, [PMID:15020712] |
| 2. Proikas-Cezanne, Tassula T and 5 more authors.. (2004) WIPI-1alpha (WIPI49), a member of the novel 7-bladed WIPI protein family, is aberrantly expressed in human cancer and is linked to starvation-induced autophagy.. Oncogene, (16): [PMID:15602573] |
| 3. Zody, Michael C MC and 73 more authors.. (2006) DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage.. Nature, (20): [PMID:16625196] |
| 4. Proikas-Cezanne, Tassula T, Ruckerbauer, Sabine S, Stierhof, York-Dieter YD, Berg, Carolin C and Nordheim, Alfred A.. (2007) Human WIPI-1 puncta-formation: a novel assay to assess mammalian autophagy.. FEBS letters, (24): [PMID:17618624] |
| 5. Vergne, Isabelle I and 7 more authors.. (2009) Control of autophagy initiation by phosphoinositide 3-phosphatase Jumpy.. The EMBO journal, (5): [PMID:19590496] |
| 6. Taguchi-Atarashi, Naoko N and 6 more authors.. (2010) Modulation of local PtdIns3P levels by the PI phosphatase MTMR3 regulates constitutive autophagy.. Traffic (Copenhagen, Denmark), [PMID:20059746] |
| 7. Gao, Ping P and 12 more authors.. (2010) The Bcl-2 homology domain 3 mimetic gossypol induces both Beclin 1-dependent and Beclin 1-independent cytoprotective autophagy in cancer cells.. The Journal of biological chemistry, (13): [PMID:20529838] |
| 8. Ho, Hsiang H, Kapadia, Rubina R, Al-Tahan, Sejad S, Ahmad, Safoora S and Ganesan, Anand K AK.. (2011) WIPI1 coordinates melanogenic gene transcription and melanosome formation via TORC1 inhibition.. The Journal of biological chemistry, (8): [PMID:21317285] |
| 9. Proikas-Cezanne, Tassula T and Robenek, Horst H.. (2011) Freeze-fracture replica immunolabelling reveals human WIPI-1 and WIPI-2 as membrane proteins of autophagosomes.. Journal of cellular and molecular medicine, [PMID:21564513] |
| 10. Mauthe, Mario and 6 more authors.. (2012) WIPI-1 Positive Autophagosome-Like Vesicles Entrap Pathogenic Staphylococcus aureus for Lysosomal Degradation.. International journal of cell biology, [PMID:22829830] |
| 11. Bakula, Daniela D and 11 more authors.. (2017) WIPI3 and WIPI4 β-propellers are scaffolds for LKB1-AMPK-TSC signalling circuits in the control of autophagy.. Nature communications, (31): [PMID:28561066] |
