Recombinant Wnt2b Antibody - Primary antibody, specific to WNT2B, Rabbit IgG
Synonyms
WNT13 | Wnt2b | XWNT2 | Protein Wnt-13 antibody | Protein Wnt-2b antibody | Wingless type MMTV integration site family, member 13 antibody | Wingless type MMTV integration site family, member 2B antibody | WNT13 antibody | Wnt2b antibody | WNT2B_HUMAN ant
Specifications & Purity
ExactAb™, Validated, Recombinant, 0.3 mg/mL
Host species
Rabbit
Specificity
WNT2B
Immunogen
A synthetic peptide derived from human Wnt2b (AA 287-300).
Rabbit anti Human Wnt2b Antibody, Recombinant, could be used for WB, ICC, IF and so on. Application: WB: 1/500-1/1000 ICC/IF: 1/50-1/200 Protein Function Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. May be involved in normal development or differentiation as well as in carcinogenesis.
Product Properties
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Immunogen affinity purified
Form
Liquid
Concentration
0.3 mg/mL
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
Recombinant Wnt2b Antibody (Ab134748) - Western Blot All lanes: Recombinant Wnt2b Antibody (Ab134748) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 44 kDa Observed band size: 45 kDa Exposure time: 1.5 s
Recombinant Wnt2b Antibody (Ab134748) - IF/ICC Immunocytochemistry analysis of Wnt2b (green) in HEK-293T cells using Recombinant Wnt2b Antibody (Ab134748) and cells were counterstained with DAPI (blue).
1.Katoh, M M, Hirai, M M, Sugimura, T T and Terada, M M.. (1996) Cloning, expression and chromosomal localization of Wnt-13, a novel member of the Wnt gene family.. Oncogene, (15):[PMID:8761309]
2.Bergstein, I I and 7 more authors.. (1997) Isolation of two novel WNT genes, WNT14 and WNT15, one of which (WNT15) is closely linked to WNT3 on human chromosome 17q21.. Genomics, (15):[PMID:9441749]
3.Katoh, M M, Kirikoshi, H H, Saitoh, T T, Sagara, N N and Koike, J J.. (2000) Alternative splicing of the WNT-2B/WNT-13 gene.. Biochemical and biophysical research communications, (18):[PMID:10944466]
4.Gregory, S G SG and 178 more authors.. (2006) The DNA sequence and biological annotation of human chromosome 1.. Nature, (18):[PMID:16710414]
5.Mihara, Emiko E and 7 more authors.. (2016) Active and water-soluble form of lipidated Wnt protein is maintained by a serum glycoprotein afamin/α-albumin.. eLife, (23):[PMID:26902720]
6.O'Connell, Amy E AE and 14 more authors.. (2018) Neonatal-Onset Chronic Diarrhea Caused by Homozygous Nonsense WNT2B Mutations.. American journal of human genetics, (5):[PMID:29909964]
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