Recombinant; Rabbit anti Human Wnt2b Antibody; WB, ICC, IF; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab134748-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $99.90 | |
| Ab134748-50μl | 50μl | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $299.90 | |
| Ab134748-100μl | 100μl | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $399.90 | |
| Ab134748-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,699.90 |
Recombinant; Rabbit anti Human Wnt2b Antibody; WB, ICC, IF; Unconjugated
| Product Name | Recombinant Wnt2b Antibody - Primary antibody, specific to WNT2B, Rabbit IgG |
|---|---|
| Synonyms | WNT13; Wnt2b; XWNT2; Protein Wnt-13 antibody; Protein Wnt-2b antibody; Wingless type MMTV integration site family, member 13 antibody; Wingless type MMTV integration site family, member 2B antibody; WNT13 antibody; Wnt2b antibody; WNT2B_HUMAN antibody; XW |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 0.3 mg/mL |
| Host species | Rabbit |
| Specificity | WNT2B |
| Immunogen | A synthetic peptide derived from human Wnt2b (AA 287-300). |
| Positive Control | WB: C6, NIH/3T3, K562, HeLa, A431 and HUVEC cell lysates. ICC/IF: 293T cells. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description | Rabbit anti Human Wnt2b Antibody, Recombinant, could be used for WB, ICC, IF and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Immunogen affinity purified |
| Form | Liquid |
| Concentration | 0.3 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant Wnt2b Antibody (Ab134748) - Western Blot
All lanes: Recombinant Wnt2b Antibody (Ab134748) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 44 kDa
Observed band size: 45 kDa
Exposure time: 1.5 s
Recombinant Wnt2b Antibody (Ab134748) - IF/ICC
Immunocytochemistry analysis of Wnt2b (green) in HEK-293T cells using Recombinant Wnt2b Antibody (Ab134748) and cells were counterstained with DAPI (blue).
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000 |
| ICC/IF |
1/50-1/200
|
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F1001223 | Certificate of Analysis | Oct 10, 2023 | Ab134748 |
| ZJ23F1001222 | Certificate of Analysis | Oct 10, 2023 | Ab134748 |
| 1. Katoh, M M, Hirai, M M, Sugimura, T T and Terada, M M.. (1996) Cloning, expression and chromosomal localization of Wnt-13, a novel member of the Wnt gene family.. Oncogene, (15): [PMID:8761309] |
| 2. Bergstein, I I and 7 more authors.. (1997) Isolation of two novel WNT genes, WNT14 and WNT15, one of which (WNT15) is closely linked to WNT3 on human chromosome 17q21.. Genomics, (15): [PMID:9441749] |
| 3. Katoh, M M, Kirikoshi, H H, Saitoh, T T, Sagara, N N and Koike, J J.. (2000) Alternative splicing of the WNT-2B/WNT-13 gene.. Biochemical and biophysical research communications, (18): [PMID:10944466] |
| 4. Gregory, S G SG and 178 more authors.. (2006) The DNA sequence and biological annotation of human chromosome 1.. Nature, (18): [PMID:16710414] |
| 5. Mihara, Emiko E and 7 more authors.. (2016) Active and water-soluble form of lipidated Wnt protein is maintained by a serum glycoprotein afamin/α-albumin.. eLife, (23): [PMID:26902720] |
| 6. O'Connell, Amy E AE and 14 more authors.. (2018) Neonatal-Onset Chronic Diarrhea Caused by Homozygous Nonsense WNT2B Mutations.. American journal of human genetics, (5): [PMID:29909964] |
