Recombinant Wnt5a Antibody - Primary antibody, specific to WNT5A, Rabbit IgG
Synonyms
WNT5A; Protein Wnt-5a; hWNT 5A antibody; hWNT5A antibody; Protein Wnt 5a antibody; Protein Wnt-5a antibody; Protein Wnt5a antibody; Wingless type MMTV integration site family member 5A antibody; Wnt 5a antibody; WNT 5A protein antibody; WNT 5A protein pre
Specifications & Purity
ExactAb™, Validated, Recombinant, 0.2 mg/mL
Host species
Rabbit
Specificity
WNT5A
Immunogen
A synthetic peptide derived from human Wnt5a (AA 340-354).
Positive Control
WB: A431 and HeLa cell lysates. ICC/IF: HeLa cells.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Product Description
Rabbit anti Human Wnt5a Antibody, Recombinant, could be used for WB, ICC, IF and so on. Application: WB: 1/500-1/1000 ICC/IF: 1/50-1/200 Protein Function Ligand for members of the frizzled family of seven transmembrane receptors. Can activate or inhibit canonical Wnt signaling, depending on receptor context. In the presence of FZD4, activates beta-catenin signaling. In the presence of ROR2, inhibits the canonical Wnt pathway by promoting beta-catenin degradation through a GSK3-independent pathway which involves down-regulation of beta-catenin-induced reporter gene expression. Suppression of the canonical pathway allows chondrogenesis to occur and inhibits tumor formation. Stimulates cell migration. Decreases proliferation, migration, invasiveness and clonogenicity of carcinoma cells and may act as a tumor suppressor. Mediates motility of melanoma cells. Required during embryogenesis for extension of the primary anterior-posterior axis and for outgrowth of limbs and the genital tubercle. Inhibits type II collagen expression in chondrocytes.
Product Properties
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Immunogen affinity purified
Form
Liquid
Concentration
0.2 mg/mL
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
Recombinant Wnt5a Antibody (Ab134765) - Western Blot All lanes: Recombinant Wnt5a Antibody (Ab134765) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 42 kDa Observed band size: 45 kDa Exposure time: 6.3 s
Recombinant Wnt5a Antibody (Ab134765) - Western Blot All lanes: Recombinant Wnt5a Antibody (Ab134765) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 42 kDa Observed band size: 45 kDa Exposure time: 1.0 s
Recombinant Wnt5a Antibody (Ab134765) - IF/ICC Immunocytochemistry analysis of Wnt5a (green) in HeLa using Recombinant Wnt5a Antibody (Ab134765), and cells were counterstained with DAPI (blue).