Recombinant Wnt5a Antibody - Primary antibody, specific to WNT5A, Rabbit IgG

    Application:
  • IF/ICC
  • WB
Features and benefits
  • Host species: Rabbit
  • Species reactivity(Reacts with): Human
  • Isotype: Rabbit IgG
  • Conjugation: Unconjugated
Item Number
Ab134765
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab134765-10μl
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$66.90
Ab134765-50μl
50μl
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$199.90
Ab134765-100μl
100μl
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$299.90
Ab134765-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,399.90

Recombinant; Rabbit anti Human Wnt5a Antibody; WB, ICC, IF; Unconjugated

Basic Description

Product NameRecombinant Wnt5a Antibody - Primary antibody, specific to WNT5A, Rabbit IgG
SynonymsWNT5A | Protein Wnt-5a | hWNT 5A antibody | hWNT5A antibody | Protein Wnt 5a antibody | Protein Wnt-5a antibody | Protein Wnt5a antibody | Wingless type MMTV integration site family member 5A antibody | Wnt 5a antibody | WNT 5A protein antibody | WNT 5A p
Specifications & PurityExactAb™, Validated, Recombinant, 0.2 mg/mL
Host speciesRabbit
SpecificityWNT5A
ImmunogenA synthetic peptide derived from human Wnt5a (AA 340-354).
Positive ControlWB: A431 and HeLa cell lysates. ICC/IF: HeLa cells.
ConjugationUnconjugated
GradeExactAb™, Recombinant, Validated
Product Description

Rabbit anti Human Wnt5a Antibody, Recombinant, could be used for WB, ICC, IF and so on.
Application:
WB: 1/500-1/1000
ICC/IF: 1/50-1/200
Protein Function
Ligand for members of the frizzled family of seven transmembrane receptors. Can activate or inhibit canonical Wnt signaling, depending on receptor context. In the presence of FZD4, activates beta-catenin signaling. In the presence of ROR2, inhibits the canonical Wnt pathway by promoting beta-catenin degradation through a GSK3-independent pathway which involves down-regulation of beta-catenin-induced reporter gene expression. Suppression of the canonical pathway allows chondrogenesis to occur and inhibits tumor formation. Stimulates cell migration. Decreases proliferation, migration, invasiveness and clonogenicity of carcinoma cells and may act as a tumor suppressor. Mediates motility of melanoma cells. Required during embryogenesis for extension of the primary anterior-posterior axis and for outgrowth of limbs and the genital tubercle. Inhibits type II collagen expression in chondrocytes.

Product Properties

IsotypeRabbit IgG
SDS-PAGE150 kDa
Purification MethodImmunogen affinity purified
FormLiquid
Concentration0.2 mg/mL
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Recombinant Wnt5a Antibody (Ab134765) - Western Blot
All lanes: Recombinant Wnt5a Antibody (Ab134765) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 42 kDa
Observed band size: 45 kDa
Exposure time: 6.3 s

Recombinant Wnt5a Antibody (Ab134765) - Western Blot
All lanes: Recombinant Wnt5a Antibody (Ab134765) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 42 kDa
Observed band size: 45 kDa
Exposure time: 1.0 s 

Recombinant Wnt5a Antibody (Ab134765) - IF/ICC
Immunocytochemistry analysis of Wnt5a (green) in HeLa using Recombinant Wnt5a Antibody (Ab134765), and cells were counterstained with DAPI (blue).

Associated Targets(Human)

WNT5A Tbio Protein Wnt-5a (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

Application

ApplicationDilution info
WB1/500-1/1000

">

1/500-1/1000

IF/ICC 1/50-1/200

">

1/50-1/200

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot NumberCertificate TypeDateItem
ZJ23F1001324Certificate of AnalysisOct 18, 2023 Ab134765
ZJ23F1001323Certificate of AnalysisOct 18, 2023 Ab134765

Related Documents

Solution Calculators