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Relatlimab (anti-LAG-3/CD223) - Primary antibody, for Flow,ELISA,Functional Assay,Kinetics (BLI),Kinetics (SPR) various applications, specific to LAG3/CD223, >95%, high purity, Human IgG4SP, Antibody of LAG3 (CD223)

≥95%(SDS-PAGE&SEC)
Lot by Lot

Features and benefits

Short Overview:
Purity>95% (SDS-PAGE&SEC); Endotoxin Level<1.0EU/mg; Human IgG4SP; CHO; ELISA, FACS, Functional assay, Animal Model; Unconjugated
Application: Flow,ELISA,Functional Assay,Kinetics (BLI),Kinetics (SPR)
Species reactivity(Reacts with): Cynomolgus monkey,Human
Isotype: Human IgG4SP

Application:

Animal Model
ELISA
Flow Cytometry
Functional Assay

Item Number

Ab169321

Grouped product items
SKU	Size	Availability	Price
Ab169321-100μg	100μg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$49.90
Ab169321-1mg	1mg	Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!	$237.90
Ab169321-5mg	5mg	Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!	$717.90
Ab169321-10mg	10mg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$1,167.90

Purity>95% (SDS-PAGE&SEC); Endotoxin Level<1.0EU/mg; Human IgG4SP; CHO; ELISA, FACS, Functional assay, Animal Model; Unconjugated

View related series

Click to view target information: LAG3

Accession#:P18627 CD223 Gene ID:3902 LAG3 LAG3 (CD223) Antibody

Basic Description

Product Name	Relatlimab (anti-LAG-3/CD223) - Primary antibody, for Flow,ELISA,Functional Assay,Kinetics (BLI),Kinetics (SPR) various applications, specific to LAG3/CD223, >95%, high purity, Human IgG4SP
Synonyms	BMS-986016 \| RELA \| CD223 antibody \| CD223 antigen antibody \| FDC protein antibody \| LAG-3 antibody \| Lag3 antibody \| LAG3_HUMAN antibody \| Lymphocyte activating 3 antibody \| Lymphocyte activation gene 3 antibody \| Lymphocyte activation gene 3 protein ant
Specifications & Purity	ExactAb™, Validated, Carrier Free, Low Endotoxin, Azide Free, Recombinant, ≥95%(SDS-PAGE&SEC), Lot by Lot
Specificity	LAG3/CD223
Application	Flow,ELISA,Functional Assay,Kinetics (BLI),Kinetics (SPR)
Conjugation	Unconjugated
Grade	Azide Free, Carrier Free, ExactAb™, Low Endotoxin, Recombinant, Validated
Action Type	INHIBITOR
Mechanism of action	Antibody of LAG3 (CD223)
Product Description	Relatlimab (anti-LAG-3/CD223) is a human LAG-3 specific monoclonal antibody that binds to LAG-3 on T cells, restoring effector function of exhausted T cells. Purity >95% (SDS-PAGE&SEC) Endotoxin Level < 1.0EU/mg

Product Properties

Antibody Type	Primary antibody
Clonality	Recombinant
Isotype	Human IgG4SP
Light Chain Type	kappa
SDS-PAGE	27.1 kDa (Light Chain) & 53.4 kDa (Heavy Chain), under reducing conditions; 176.6 kDa, under non-reducing conditions.
Purification Method	Protein A purified
Purity	>95%
Source	CHO supernatant
Form	Liquid
Concentration	Lot by Lot
Storage Temp	Store at -80°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at -80℃ for 24 months. Upon delivery aliquot. Avoid freeze/thaw cycle.
CAS	1673516-98-7

Images

Relatlimab (anti-LAG-3/CD223) (Ab169321) - Flow Cytometry
Flow Cytometry analysis of PHA-stimulated (3 days) human peripheral blood mononuclear lymphocytes labelling LAG-3/CD223 (red) with Relatlimab (anti- LAG-3/CD223) (Ab169321). Goat Anti-Human IgG (PE) (Ab175838) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, human IgG (Ab170213). Black - Unlabelled control, cells without incubation with primary antibody.

Relatlimab (anti-LAG-3/CD223) (Ab169321) - SEC
The purity of Relatlimab (anti-LAG-3/CD223) (Ab169321) is more than 95% verified by HPLC.

Relatlimab (anti-LAG-3/CD223) (Ab169321) - ELISA
Immobilized Recombinant Human LAG-3 protein (rp176292) at 1.0 μg/mL can bind Relatlimab (anti-LAG-3/CD223) (Ab169321) with the EC₅₀ of 41.53 ng/mL.

Associated Targets(Human)

LAG3 Tclin Lymphocyte activation gene 3 protein (0 Activities)

Discover Target: LAG3

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

LAG3 Tclin Lymphocyte activation gene 3 protein (1 Activities)

Discover Target: LAG3

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot Number	Certificate Type	Date	Item
ZJ23F1001433	Certificate of Analysis	Oct 26, 2023	Ab169321
ZJ23F1001416	Certificate of Analysis	Oct 26, 2023	Ab169321

References

1. Huang CT, Workman CJ, Flies D, Pan X, Marson AL, Zhou G, Hipkiss EL, Ravi S, Kowalski J, Levitsky HI et al.. (2004) Role of LAG-3 in regulatory T cells.. Immunity, 21 (4): (503-13). [PMID:15485628]
2. Nguyen LT, Ohashi PS. (2015) Clinical blockade of PD1 and LAG3--potential mechanisms of action.. Nat Rev Immunol, 15 (1): (45-56). [PMID:25534622]
3. Triebel F, Jitsukawa S, Baixeras E, Roman-Roman S, Genevee C, Viegas-Pequignot E, Hercend T. (1990) LAG-3, a novel lymphocyte activation gene closely related to CD4.. J Exp Med, 171 (5): (1393-405). [PMID:1692078]
4. Baixeras, E E and 8 more authors.. (1992) Characterization of the lymphocyte activation gene 3-encoded protein. A new ligand for human leukocyte antigen class II antigens.. The Journal of experimental medicine, (1): [PMID:1380059]
5. Huard, B B and 8 more authors.. (1997) Characterization of the major histocompatibility complex class II binding site on LAG-3 protein.. Proceedings of the National Academy of Sciences of the United States of America, (27): [PMID:9159144]
6. Bruniquel, D D, Borie, N N, Hannier, S S and Triebel, F F.. (1998) Regulation of expression of the human lymphocyte activation gene-3 (LAG-3) molecule, a ligand for MHC class II.. Immunogenetics, [PMID:9634475]
7. Scherer, Steven E SE and 216 more authors.. (2006) The finished DNA sequence of human chromosome 12.. Nature, (16): [PMID:16541075]
8. Camisaschi, Chiara C and 9 more authors.. (2010) LAG-3 expression defines a subset of CD4(+)CD25(high)Foxp3(+) regulatory T cells that are expanded at tumor sites.. Journal of immunology (Baltimore, Md. : 1950), (1): [PMID:20421648]
9. Wang, Jun and 20 more authors.. (2019) Fibrinogen-like Protein 1 Is a Major Immune Inhibitory Ligand of LAG-3.. Cell, (10): [PMID:30580966]

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