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S100B Mouse mAb - Primary antibody, specific to S100b , Mouse IgG1

Lot by Lot

Application:

Capture Antibody
CLIA
Detection Antibody
ELISA
FIA
GICA
LF

Features and benefits

Host species: Mouse
Species reactivity(Reacts with): Human
Isotype: Mouse IgG1
Clone Number: 22G7-5
Conjugation: Unconjugated

Item Number

Ab008407

Grouped product items
SKU	Size	Availability	Price
Ab008407-10μg	10μg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$66.90
Ab008407-50μg	50μg	Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!	$199.90
Ab008407-100μg	100μg	Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!	$299.90
Ab008407-1mg	1mg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$999.90

mAb(22G7-5); Mouse anti Human S100B Antibody; Detection or Capture Antibody, ELISA, CLIA, LF, GICA, FIA; Unconjugated

View related series

Click to view target information: S100B

Accession#:P04271 Gene ID:6285 S100B

Basic Description

Product Name	S100B Mouse mAb - Primary antibody, specific to S100b , Mouse IgG1
Synonyms	S100 calcium-binding protein B \| S-100 protein beta chain \| S-100 protein subunit beta
Specifications & Purity	ExactAb™, Validated, Carrier Free, Azide Free, Lot by Lot
Host species	Mouse
Specificity	S100b
Immunogen	Recombinant Human S100B Protein, C-His tag
Conjugation	Unconjugated
Grade	Azide Free, Carrier Free, ExactAb™, Validated

Product Properties

Isotype	Mouse IgG1
SDS-PAGE	25.6 kDa (Light Chain) & 52.1 kDa (Heavy Chain), under reducing conditions; 174.5 kDa, under non-reducing conditions.
Purification Method	Affinity chromatography
Matched Pairs	[Ab008408] Monoclonal S100B Antibody as Detection or Capture antibody in ELISA, CLIA, LF, GICA, FIA
Form	Purified polyclonal antibodies Antibody
Concentration	Lot by Lot
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at 37°C short term（7 days）Store at 4°C short term（30 days）. Store at -20±5°C long term (36 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Sandwich ELISA - S100B protein standard curve
S100B protein was serially diluted 3-fold and captured by S100B Mouse mAb (Ab008408) coated on a Clear Polystyrene Microplate (W291037). S100B Mouse mAb (Ab008407) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (np156148).
Sensitivity: 30.3 pg/mL
Range: 0.1 ng/mL - 3.2 ng/mL

Sandwich ELISA - S100B protein standard curve
S100B protein was serially diluted 3-fold and captured by S100B Mouse mAb (Ab008407) coated on a Clear Polystyrene Microplate (W291037). S100B Mouse mAb (Ab008408) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (np156148).
Sensitivity: 18.8 pg/mL
Range: 0.13 ng/mL - 3.1 ng/mL

S100B Mouse mAb (Ab008407) - SDS-PAGE
3 μg/lane of S100B Mouse mAb (Ab008407) was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the band at 26.0 kDa (Light Chain) & 54.2 kDa (Heavy Chain) under reducing conditions and 178.7 kDa under non-reducing conditions.

Relevance of detection results between aladdin Antibody Pair (Ab008408 & Ab008407) and Roche in CLIA
·Capture antibody: S100B Mouse mAb (Ab008408)
·Detection antibody: S100B Mouse mAb (Ab008407)

Application

Dilution info

CLIA

Capture: 20µg/2mg magnetic beads; Detection: 100ug/4ug AE

FLFIA

Capture: Fluorescent beads/Antibody = 10/1; Detection: 1.0 mg/mL

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot Number	Certificate Type	Date	Item
ZJ23F0901143	Certificate of Analysis	Sep 24, 2023	Ab008407
ZJ23F0901142	Certificate of Analysis	Sep 24, 2023	Ab008407

References

1. Gerhard DS, Wagner L, Feingold EA, Shenmen CM, Grouse LH, Schuler G, Klein SL, Old S, Rasooly R, Good P et al.. (2004) The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).. Genome Res, 14 (10B): (2121-7). [PMID:15489334] [10.1021/op500134e]
2. Markowitz J, Chen I, Gitti R, Baldisseri DM, Pan Y, Udan R, Carrier F, MacKerell AD, Weber DJ.. (2004) Identification and characterization of small molecule inhibitors of the calcium-dependent S100B-p53 tumor suppressor interaction.. J Med Chem, 47 (21): (5085-5093). [PMID:15456252] [10.1021/jm0497038]
3. Yoshimura C, Miyafusa T, Tsumoto K.. (2013) Identification of small-molecule inhibitors of the human S100B-p53 interaction and evaluation of their activity in human melanoma cells.. Bioorg Med Chem, 21 (5): (1109-1115). [PMID:23375094] [10.1016/j.bmc.2012.12.042]
4. Allore, R J RJ and 6 more authors.. (1990) Cloning and expression of the human S100 beta gene.. The Journal of biological chemistry, (15): [PMID:2394738]
5. Jensen, R R, Marshak, D R DR, Anderson, C C, Lukas, T J TJ and Watterson, D M DM.. (1985) Characterization of human brain S100 protein fraction: amino acid sequence of S100 beta.. Journal of neurochemistry, [PMID:4031854]
6. Baudier, J J, Glasser, N N, Haglid, K K and Gerard, D D.. (1984) Purification, characterization and ion binding properties of human brain S100b protein.. Biochimica et biophysica acta, (23): [PMID:6487634]
7. Smith, S P SP and Shaw, G S GS.. (1998) A novel calcium-sensitive switch revealed by the structure of human S100B in the calcium-bound form.. Structure (London, England : 1993), (15): [PMID:9519411]
8. Yang, Q Q, O'Hanlon, D D, Heizmann, C W CW and Marks, A A.. (1999) Demonstration of heterodimer formation between S100B and S100A6 in the yeast two-hybrid system and human melanoma.. Experimental cell research, (1): [PMID:9925766]
9. Hattori, M M and 64 more authors.. (2000) The DNA sequence of human chromosome 21.. Nature, (18): [PMID:10830953]
10. McClintock, Kimberly A KA and Shaw, Gary S GS.. (2003) A novel S100 target conformation is revealed by the solution structure of the Ca2+-S100B-TRTK-12 complex.. The Journal of biological chemistry, (21): [PMID:12480931]
11. Lee, Tih-Shih TS and 12 more authors.. () Gene expression in temporal lobe epilepsy is consistent with increased release of glutamate by astrocytes.. Molecular medicine (Cambridge, Mass.), [PMID:17515952]
12. Marlatt, Nicole M NM and Shaw, Gary S GS.. (2007) Amide exchange shows calcium-induced conformational changes are transmitted to the dimer interface of S100B.. Biochemistry, (26): [PMID:17536784]
13. Liang, Jie J and 15 more authors.. (2007) Differential expression of calcium-related genes in gastric cancer cells transfected with cellular prion protein.. Biochemistry and cell biology = Biochimie et biologie cellulaire, [PMID:17612632]
14. Friel, Lara A LA and 9 more authors.. (2007) The calcium binding protein, S100B, is increased in the amniotic fluid of women with intra-amniotic infection/inflammation and preterm labor with intact or ruptured membranes.. Journal of perinatal medicine, [PMID:17624933]
15. Gilquin, Benoît B and 11 more authors.. (2010) The calcium-dependent interaction between S100B and the mitochondrial AAA ATPase ATAD3A and the role of this complex in the cytoplasmic processing of ATAD3A.. Molecular and cellular biology, [PMID:20351179]
16. Park, Hajeung H, Adsit, Floyd G FG and Boyington, Jeffrey C JC.. (2010) The 1.5 Å crystal structure of human receptor for advanced glycation endproducts (RAGE) ectodomains reveals unique features determining ligand binding.. The Journal of biological chemistry, (24): [PMID:20943659]
17. Ostendorp, Thorsten T, Diez, Joachim J, Heizmann, Claus W CW and Fritz, Günter G.. (2011) The crystal structures of human S100B in the zinc- and calcium-loaded state at three pH values reveal zinc ligand swapping.. Biochimica et biophysica acta, [PMID:20950652]
18. Yamaguchi, Fuminori F and 6 more authors.. (2012) S100 proteins modulate protein phosphatase 5 function: a link between CA2+ signal transduction and protein dephosphorylation.. The Journal of biological chemistry, (20): [PMID:22399290]

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