Grouped product items

SKU

Size

Availability

Price

Qty

Ab008408-10μg

10μg

Available within 8-12 weeks(?)

$66.90

Ab008408-50μg

50μg

Available within 4-8 weeks(?)

$199.90

Ab008408-100μg

100μg

Available within 4-8 weeks(?)

$299.90

Ab008408-1mg

1mg

Available within 8-12 weeks(?)

$999.90

Basic Description

Product Name	S100B Mouse mAb - Primary antibody, specific to S100b , Mouse IgG1
Synonyms	S100 calcium-binding protein B \| S-100 protein beta chain \| S-100 protein subunit beta
Specifications & Purity	ExactAb™, Validated, Carrier Free, Azide Free, Lot by Lot
Host species	Mouse
Specificity	S100b
Immunogen	Recombinant Human S100B Protein, C-His tag
Conjugation	Unconjugated
Grade	Azide Free, Carrier Free, ExactAb™, Validated
Product Description	Mouse anti Human S100B Antibody, Monoclonal (5H2-3), is a Detection or Capture antibody could be used for ELISA, CLIA, LF, GICA, FIA. CLIA: Capture: 20µg/2mg magnetic beads; Detection: 100µg/4µg AE FLFIA: Capture: Fluorescent beads/Antibody = 10/1; Detection: 1.0 mg/mL

Product Properties

Isotype	Mouse IgG1
SDS-PAGE	26.0 kDa (Light Chain) & 46.1 kDa (Heavy Chain), under reducing conditions; 178.7 kDa, under non-reducing conditions.
Purification Method	Affinity chromatography
Matched Pairs	[Ab008407] Monoclonal S100B Antibody as Capture or Detection antibody in ELISA, CLIA, LF, GICA, FIA
Form	Liquid
Concentration	Lot by Lot
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at 37°C short term (7 days). Store at 4°C short term (30 days). Store at -20±5°C long term (36 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Sandwich ELISA - S100B protein standard curve
S100B protein was serially diluted 3-fold and captured by S100B Mouse mAb (Ab008408) coated on a Clear Polystyrene Microplate (W291037). S100B Mouse mAb (Ab008407) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (np156148).
Sensitivity: 30.3 pg/mL
Range: 0.1 ng/mL - 3.2 ng/mL

Sandwich ELISA - S100B protein standard curve
S100B protein was serially diluted 3-fold and captured by S100B Mouse mAb (Ab008407) coated on a Clear Polystyrene Microplate (W291037). S100B Mouse mAb (Ab008408) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (np156148).
Sensitivity: 18.8 pg/mL
Range: 0.13 ng/mL - 3.1 ng/mL

Relevance of detection results between aladdin Antibody Pair (Ab008408 & Ab008407) and Roche in CLIA
·Capture antibody: S100B Mouse mAb (Ab008408)
·Detection antibody: S100B Mouse mAb (Ab008407)

Associated Targets(Human)

S100B Tchem Protein S100-B (0 Activities)

Discover Target: S100B

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mechanisms of Action

Mechanism of Action	Action Type	target ID	Target Name	Target Type	Target Organism	Binding Site Name	References

Application

Application	Dilution info
CLIA	Capture: 20µg/2mg magnetic beads; Detection: 100ug/4ug AE
FLFIA	Capture: Fluorescent beads/Antibody = 10/1; Detection: 1.0 mg/mL

Certificates

Certificate of Analysis(COA)

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Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot Number	Certificate Type	Date	Item
ZJ23F0901028	Certificate of Analysis	Sep 18, 2023	Ab008408
ZJ23F0901026	Certificate of Analysis	Sep 18, 2023	Ab008408

FAQs and Articles

FAQ

Immunohistochemistry staining with samples in paraffin (IHC-P)

Categories: Protocols

Tags:

Monoclonal antibody Paraffin Section Immunohistochemistry/IHC

RNA dot blot protocol

Categories: Protocols

Tags:

Protein Electrophoresis & Western Blotting Dot Blotting Primary antibodies Secondary Antibodies

References

1. Gerhard DS, Wagner L, Feingold EA, Shenmen CM, Grouse LH, Schuler G, Klein SL, Old S, Rasooly R, Good P et al.. (2004) The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).. Genome Res, 14 (10B): (2121-7). [PMID:15489334] [10.1021/op500134e]
2. Markowitz J, Chen I, Gitti R, Baldisseri DM, Pan Y, Udan R, Carrier F, MacKerell AD, Weber DJ.. (2004) Identification and characterization of small molecule inhibitors of the calcium-dependent S100B-p53 tumor suppressor interaction.. J Med Chem, 47 (21): (5085-5093). [PMID:15456252] [10.1021/jm0497038]
3. Yoshimura C, Miyafusa T, Tsumoto K.. (2013) Identification of small-molecule inhibitors of the human S100B-p53 interaction and evaluation of their activity in human melanoma cells.. Bioorg Med Chem, 21 (5): (1109-1115). [PMID:23375094] [10.1016/j.bmc.2012.12.042]
4. Allore, R J RJ and 6 more authors.. (1990) Cloning and expression of the human S100 beta gene.. The Journal of biological chemistry, (15): [PMID:2394738]
5. Jensen, R R, Marshak, D R DR, Anderson, C C, Lukas, T J TJ and Watterson, D M DM.. (1985) Characterization of human brain S100 protein fraction: amino acid sequence of S100 beta.. Journal of neurochemistry, [PMID:4031854]
6. Baudier, J J, Glasser, N N, Haglid, K K and Gerard, D D.. (1984) Purification, characterization and ion binding properties of human brain S100b protein.. Biochimica et biophysica acta, (23): [PMID:6487634]
7. Smith, S P SP and Shaw, G S GS.. (1998) A novel calcium-sensitive switch revealed by the structure of human S100B in the calcium-bound form.. Structure (London, England : 1993), (15): [PMID:9519411]
8. Yang, Q Q, O'Hanlon, D D, Heizmann, C W CW and Marks, A A.. (1999) Demonstration of heterodimer formation between S100B and S100A6 in the yeast two-hybrid system and human melanoma.. Experimental cell research, (1): [PMID:9925766]
9. Hattori, M M and 64 more authors.. (2000) The DNA sequence of human chromosome 21.. Nature, (18): [PMID:10830953]
10. McClintock, Kimberly A KA and Shaw, Gary S GS.. (2003) A novel S100 target conformation is revealed by the solution structure of the Ca2+-S100B-TRTK-12 complex.. The Journal of biological chemistry, (21): [PMID:12480931]
11. Lee, Tih-Shih TS and 12 more authors.. () Gene expression in temporal lobe epilepsy is consistent with increased release of glutamate by astrocytes.. Molecular medicine (Cambridge, Mass.), [PMID:17515952]
12. Marlatt, Nicole M NM and Shaw, Gary S GS.. (2007) Amide exchange shows calcium-induced conformational changes are transmitted to the dimer interface of S100B.. Biochemistry, (26): [PMID:17536784]
13. Liang, Jie J and 15 more authors.. (2007) Differential expression of calcium-related genes in gastric cancer cells transfected with cellular prion protein.. Biochemistry and cell biology = Biochimie et biologie cellulaire, [PMID:17612632]
14. Friel, Lara A LA and 9 more authors.. (2007) The calcium binding protein, S100B, is increased in the amniotic fluid of women with intra-amniotic infection/inflammation and preterm labor with intact or ruptured membranes.. Journal of perinatal medicine, [PMID:17624933]
15. Gilquin, Benoît B and 11 more authors.. (2010) The calcium-dependent interaction between S100B and the mitochondrial AAA ATPase ATAD3A and the role of this complex in the cytoplasmic processing of ATAD3A.. Molecular and cellular biology, [PMID:20351179]
16. Park, Hajeung H, Adsit, Floyd G FG and Boyington, Jeffrey C JC.. (2010) The 1.5 Å crystal structure of human receptor for advanced glycation endproducts (RAGE) ectodomains reveals unique features determining ligand binding.. The Journal of biological chemistry, (24): [PMID:20943659]
17. Ostendorp, Thorsten T, Diez, Joachim J, Heizmann, Claus W CW and Fritz, Günter G.. (2011) The crystal structures of human S100B in the zinc- and calcium-loaded state at three pH values reveal zinc ligand swapping.. Biochimica et biophysica acta, [PMID:20950652]
18. Yamaguchi, Fuminori F and 6 more authors.. (2012) S100 proteins modulate protein phosphatase 5 function: a link between CA2+ signal transduction and protein dephosphorylation.. The Journal of biological chemistry, (20): [PMID:22399290]

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S100B Mouse mAb - Primary antibody, specific to S100b , Mouse IgG1