Sabatolimab (anti-TIM-3) - Primary antibody, for ELISA,Functional Assay,Flow cytometry,Kinetics (BLI),Kinetics (SPR) various applications, specific to TIM-3, >95%, high purity, Human IgG4SP, Antibody of TIM3 (CD366)

    Application:
  • Animal Model
  • ELISA
  • Flow Cytometry
  • Functional Assay
Features and benefits
  • Host species: Human
  • Species reactivity(Reacts with): Cynomolgus monkey,Human
  • Isotype: Human IgG4SP
  • Conjugation: Unconjugated
Item Number
Ab169315
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab169315-100μg
100μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$39.90
Ab169315-1mg
1mg
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$177.90
Ab169315-5mg
5mg
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$507.90
Ab169315-10mg
10mg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$867.90

Purity>95% (SDS-PAGE&SEC); Endotoxin Level<1.0EU/mg; Human IgG4SP; CHO; ELISA, FACS, Functional assay, Animal Model; Unconjugated

Basic Description

Product NameSabatolimab (anti-TIM-3) - Primary antibody, for ELISA,Functional Assay,Flow cytometry,Kinetics (BLI),Kinetics (SPR) various applications, specific to TIM-3, >95%, high purity, Human IgG4SP
SynonymsHAVcr-2 | T-cell immunoglobulin and mucin domain-containing protein 3 | T-cell immunoglobulin mucin receptor 3 | T-cell membrane protein 3 | TIM-3 | TIMD-3
Specifications & PurityExactAb™, Validated, Carrier Free, Low Endotoxin, Azide Free, Recombinant, ≥95%(SDS-PAGE&SEC), Lot by Lot
Host speciesHuman
SpecificityTIM-3
ApplicationELISA,Functional Assay,Flow cytometry,Kinetics (BLI),Kinetics (SPR)
ConjugationUnconjugated
GradeAzide Free, Carrier Free, ExactAb™, Low Endotoxin, Recombinant, Validated
Action TypeINHIBITOR
Mechanism of actionAntibody of TIM3 (CD366)
Product Description

Sabatolimab is a high-affinity, humanized, IgG4 (S228P) antibody targeting TIM-3, an inhibitory receptor that regulates adaptive and innate immune responses. Sabatolimab is a potential immunosuppression agent that can target TIM-3 on immune and myeloid cells
Purity
>95% (SDS-PAGE&SEC)
Endotoxin Level
< 1.0EU/mg

Product Properties

Antibody TypePrimary antibody
ClonalityRecombinant
IsotypeHuman IgG4SP
Light Chain Typekappa
SDS-PAGE26.4 kDa (Light Chain) & 51.8 kDa (Heavy Chain), under reducing conditions; 179.3 kDa, under non-reducing conditions.
Purification MethodProtein A purified
Purity>95%
SourceCHO supernatant
FormLiquid
ConcentrationLot by Lot
Storage TempStore at -80°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at -80℃ for 24 months. Upon delivery aliquot. Avoid freeze/thaw cycle.
CAS2252262-24-9

Images

Sabatolimab (anti-TIM-3) (Ab169315) - Flow Cytometry
Flow Cytometry analysis of PHA-stimulated (3 days) human peripheral blood mononuclear lymphocytes labelling TIM-3 (red) with Sabatolimab (anti-TIM-3) (Ab169315). Goat Anti-Human IgG (PE) (Ab175838) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, human IgG (Ab170213). Black - Unlabelled control, cells without incubation with primary antibody.

Sabatolimab (anti-TIM-3) (Ab169315) - SEC
The purity of Sabatolimab (anti-TIM-3) (Ab169315) is more than 95% verified by HPLC.

Associated Targets(Human)

HAVCR2 Tchem Hepatitis A virus cellular receptor 2 (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

Mechanisms of Action

Mechanism of ActionAction Typetarget IDTarget NameTarget TypeTarget OrganismBinding Site NameReferences

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot NumberCertificate TypeDateItem
ZJ23F0900968Certificate of AnalysisSep 06, 2023 Ab169315
ZJ23F0900967Certificate of AnalysisSep 06, 2023 Ab169315

Related Documents

References

1. Schwartz S, Patel N, Longmire T, Jayaraman P, Jiang X, Lu H, Baker L, Velez J, Ramesh R, Wavreille AS et al..  (2022)  Characterization of sabatolimab, a novel immunotherapy with immuno-myeloid activity directed against TIM-3 receptor..  Immunother Adv,  (1): (ltac019).  [PMID:36196369] [10.1021/op500134e]
2. Barth S, Naran K.  (2022)  TIM-3: a tumor-associated antigen beyond checkpoint inhibition?.  Immunother Adv,  (1): (ltac021).  [PMID:36406467] [10.1021/op500134e]
3. Monney L, Sabatos CA, Gaglia JL, Ryu A, Waldner H, Chernova T, Manning S, Greenfield EA, Coyle AJ, Sobel RA et al..  (2002)  Th1-specific cell surface protein Tim-3 regulates macrophage activation and severity of an autoimmune disease..  Nature,  415  (6871): (536-41).  [PMID:11823861] [10.1021/op500134e]
4. Zhu C, Anderson AC, Schubart A, Xiong H, Imitola J, Khoury SJ, Zheng XX, Strom TB, Kuchroo VK.  (2005)  The Tim-3 ligand galectin-9 negatively regulates T helper type 1 immunity..  Nat Immunol,  (12): (1245-52).  [PMID:16286920] [10.1021/op500134e]
5. Sánchez-Fueyo, Alberto A and 12 more authors..  (2003)  Tim-3 inhibits T helper type 1-mediated auto- and alloimmune responses and promotes immunological tolerance..  Nature immunology,      [PMID:14556005]
6. van de Weyer, Philipp S PS and 5 more authors..  (2006)  A highly conserved tyrosine of Tim-3 is phosphorylated upon stimulation by its ligand galectin-9..  Biochemical and biophysical research communications,    (15):   [PMID:17069754]
7. Anderson, Ana C AC and 13 more authors..  (2007)  Promotion of tissue inflammation by the immune receptor Tim-3 expressed on innate immune cells..  Science (New York, N.Y.),    (16):   [PMID:18006747]
8. Jones, R Brad RB and 26 more authors..  (2008)  Tim-3 expression defines a novel population of dysfunctional T cells with highly elevated frequencies in progressive HIV-1 infection..  The Journal of experimental medicine,    (24):   [PMID:19001139]
9. Golden-Mason, Lucy L and 9 more authors..  (2009)  Negative immune regulator Tim-3 is overexpressed on T cells in hepatitis C virus infection and its blockade rescues dysfunctional CD4+ and CD8+ T cells..  Journal of virology,      [PMID:19587053]
10. DeKruyff, Rosemarie H RH and 11 more authors..  (2010)  T cell/transmembrane, Ig, and mucin-3 allelic variants differentially recognize phosphatidylserine and mediate phagocytosis of apoptotic cells..  Journal of immunology (Baltimore, Md. : 1950),    (15):   [PMID:20083673]
11. Gleason, Michelle K MK and 13 more authors..  (2012)  Tim-3 is an inducible human natural killer cell receptor that enhances interferon gamma production in response to galectin-9..  Blood,    (29):   [PMID:22323453]
12. Ndhlovu, Lishomwa C LC and 9 more authors..  (2012)  Tim-3 marks human natural killer cell maturation and suppresses cell-mediated cytotoxicity..  Blood,    (19):   [PMID:22383801]
13. Leitner, Judith and 5 more authors..  (2013)  TIM-3 does not act as a receptor for galectin-9..  PLoS pathogens,      [PMID:23555261]
14. Clayton, Kiera L KL and 6 more authors..  (2014)  T cell Ig and mucin domain-containing protein 3 is recruited to the immune synapse, disrupts stable synapse formation, and associates with receptor phosphatases..  Journal of immunology (Baltimore, Md. : 1950),    (15):   [PMID:24337741]
15. Gorman, Jacob V JV and Colgan, John D JD..  (2014)  Regulation of T cell responses by the receptor molecule Tim-3..  Immunologic research,      [PMID:24825777]
16. Gautron, Anne-Sophie AS, Dominguez-Villar, Margarita M, de Marcken, Marine M and Hafler, David A DA..  (2014)  Enhanced suppressor function of TIM-3+ FoxP3+ regulatory T cells..  European journal of immunology,      [PMID:24838857]
17. Tomkowicz, Brian B and 11 more authors..  (2015)  TIM-3 Suppresses Anti-CD3/CD28-Induced TCR Activation and IL-2 Expression through the NFAT Signaling Pathway..  PloS one,      [PMID:26492563]
18. Gayden, Tenzin T and 43 more authors..  (2018)  Germline HAVCR2 mutations altering TIM-3 characterize subcutaneous panniculitis-like T cell lymphomas with hemophagocytic lymphohistiocytic syndrome..  Nature genetics,      [PMID:30374066]
19. Gayden, Tenzin T and 43 more authors..  (2019)  Author Correction: Germline HAVCR2 mutations altering TIM-3 characterize subcutaneous panniculitis-like T cell lymphomas with hemophagocytic lymphohistiocytic syndrome..  Nature genetics,      [PMID:30429576]
20. Polprasert, Chantana C and 25 more authors..  (2019)  Frequent germline mutations of HAVCR2 in sporadic subcutaneous panniculitis-like T-cell lymphoma..  Blood advances,    (26):   [PMID:30792187]

Solution Calculators