| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab126811-10μl | 10μl | In stock | $69.90 | |
| Ab126811-50μl | 50μl | In stock | $199.90 | |
| Ab126811-100μl | 100μl | In stock | $309.90 | |
| Ab126811-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,399.90 |
pAb; Rabbit anti Human Scramblase 1 Antibody; WB; Unconjugated
| Product Name | Scramblase 1 Antibody - Primary antibody, specific to PLSCR1, Rabbit IgG |
|---|---|
| Synonyms | 3.1.-.- | Ca(2+)-dependent phospholipid scramblase 1 | Erythrocyte phospholipid scramblase | Mg(2+)-dependent nuclease | MmTRA1b | PL scramblase 1 | Ca(2+) dependent phospholipid scramblase 1 antibody | Ca(2+)-dependent phospholipid scramblase 1 antibody |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, Azide Free, High performance, Lot by Lot |
| Host species | Rabbit |
| Specificity | PLSCR1 |
| Immunogen | Recombinant PLSCR1 expressed in E.coli (AA 1-318) |
| Positive Control | WB: A431, K562, Hep G2 and HEK293 cell lysates. |
| Conjugation | Unconjugated |
| Grade | Azide Free, Carrier Free, ExactAb™, High Performance, Validated |
| Product Description |
Rabbit anti Human Scramblase 1 Antibody, Polyclonal, could be used for WB and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Antigen affinity purified + Protein A purified |
| Form | Liquid |
| Concentration | Lot by Lot |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Scramblase 1 Antibody (Ab126811) - Western Blot
All lanes: Scramblase 1 Antibody (Ab126811) at 1/5000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution
Predicted band size: 35 kDa
Observed band size: 34 kDa
Exposure time: 16.4 s
Scramblase 1 Antibody (Ab126811) - Western Blot
All lanes: Scramblase 1 Antibody (Ab126811) at 1/5000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution
Predicted band size: 35 kDa
Observed band size: 34 kDa
Exposure time: 1.0 s
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB |
0.01-2 µg/mL
|
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0404445 | Certificate of Analysis | Apr 11, 2024 | Ab126811 |
| ZJ24F0404446 | Certificate of Analysis | Apr 11, 2024 | Ab126811 |
| ZJ24F0404447 | Certificate of Analysis | Apr 11, 2024 | Ab126811 |
| 1. Vincent G Francis,Abdul M Mohammed,Gopala K Aradhyam,Sathyanarayana N Gummadi. (2013-04-18) The single C-terminal helix of human phospholipid scramblase 1 is required for membrane insertion and scrambling activity.. The FEBS journal, 280 ((12)): (2855-2869). [PMID:23590222] |
| 2. Cecile Herate,Ghania Ramdani,Nancy J Grant,Sabrina Marion,Stephane Gasman,Florence Niedergang,Serge Benichou,Jerome Bouchet. (2016-01-09) Phospholipid Scramblase 1 Modulates FcR-Mediated Phagocytosis in Differentiated Macrophages.. PloS one, 11 ((1)): (e0145617-e0145617). [PMID:26745724] |
| 3. Bassé, F F, Stout, J G JG, Sims, P J PJ and Wiedmer, T T.. (1996) Isolation of an erythrocyte membrane protein that mediates Ca2+-dependent transbilayer movement of phospholipid.. The Journal of biological chemistry, (19): [PMID:8663431] |
| 4. Zhou, Q Q and 5 more authors.. (1997) Molecular cloning of human plasma membrane phospholipid scramblase. A protein mediating transbilayer movement of plasma membrane phospholipids.. The Journal of biological chemistry, (18): [PMID:9218461] |
| 5. Zhou, Q Q, Sims, P J PJ and Wiedmer, T T.. (1998) Identity of a conserved motif in phospholipid scramblase that is required for Ca2+-accelerated transbilayer movement of membrane phospholipids.. Biochemistry, (24): [PMID:9485382] |
| 6. Zhao, J J, Zhou, Q Q, Wiedmer, T T and Sims, P J PJ.. (1998) Palmitoylation of phospholipid scramblase is required for normal function in promoting Ca2+-activated transbilayer movement of membrane phospholipids.. Biochemistry, (5): [PMID:9572851] |
| 7. Kasukabe, T T, Kobayashi, H H, Kaneko, Y Y, Okabe-Kado, J J and Honma, Y Y.. (1998) Identity of human normal counterpart (MmTRA1b) of mouse leukemogenesis-associated gene (MmTRA1a) product as plasma membrane phospholipid scramblase and chromosome mapping of the human MmTRA1b/phospholipid scramblase gene.. Biochemical and biophysical research communications, (19): [PMID:9712717] |
| 8. Frasch, S C SC and 6 more authors.. (2000) Regulation of phospholipid scramblase activity during apoptosis and cell activation by protein kinase Cdelta.. The Journal of biological chemistry, (28): [PMID:10770950] |
| 9. Wiedmer, T T, Zhou, Q Q, Kwoh, D Y DY and Sims, P J PJ.. (2000) Identification of three new members of the phospholipid scramblase gene family.. Biochimica et biophysica acta, (31): [PMID:10930526] |
| 10. Sun, J J and 5 more authors.. (2001) c-Abl tyrosine kinase binds and phosphorylates phospholipid scramblase 1.. The Journal of biological chemistry, (3): [PMID:11390389] |
| 11. Wiedmer, Therese T, Zhao, Ji J, Nanjundan, Meera M and Sims, Peter J PJ.. (2003) Palmitoylation of phospholipid scramblase 1 controls its distribution between nucleus and plasma membrane.. Biochemistry, (11): [PMID:12564925] |
| 12. Dong, Beihua B and 12 more authors.. (2004) Phospholipid scramblase 1 potentiates the antiviral activity of interferon.. Journal of virology, [PMID:15308695] |
| 13. Chen, Min-Hsuan MH and 5 more authors.. (2005) Phospholipid scramblase 1 contains a nonclassical nuclear localization signal with unique binding site in importin alpha.. The Journal of biological chemistry, (18): [PMID:15611084] |
| 14. Zhou, Quansheng Q and 5 more authors.. (2005) Phospholipid scramblase 1 binds to the promoter region of the inositol 1,4,5-triphosphate receptor type 1 gene to enhance its expression.. The Journal of biological chemistry, (14): [PMID:16091359] |
| 15. Muzny, Donna M DM and 113 more authors.. (2006) The DNA sequence, annotation and analysis of human chromosome 3.. Nature, (27): [PMID:16641997] |
| 16. Wyles, Jessica P JP, Wu, Zhongqin Z, Mirski, Shelagh E L SE and Cole, Susan P C SP.. (2007) Nuclear interactions of topoisomerase II alpha and beta with phospholipid scramblase 1.. Nucleic acids research, [PMID:17567603] |
| 17. Sahu, Santosh Kumar SK, Gopala Krishna, A A and Gummadi, Sathyanarayana N SN.. (2008) Over-expression of recombinant human phospholipid scramblase 1 in E. coli and its purification from inclusion bodies.. Biotechnology letters, [PMID:18629440] |
| 18. Gong, Qiaoling Q and 10 more authors.. (2011) Phospholipid scramblase 1 mediates hepatitis C virus entry into host cells.. FEBS letters, (2): [PMID:21806988] |
| 19. Cusick, John K JK, Mustian, Andrea A, Jacobs, Aaron T AT and Reyland, Mary E ME.. (2012) Identification of PLSCR1 as a protein that interacts with RELT family members.. Molecular and cellular biochemistry, [PMID:22052202] |
| 20. Shettihalli, Ashok Kumar AK and Gummadi, Sathyanarayana N SN.. (2013) Biochemical evidence for lead and mercury induced transbilayer movement of phospholipids mediated by human phospholipid scramblase 1.. Chemical research in toxicology, (17): [PMID:23659204] |
| 21. Sánchez-Magraner, Lissete L and 8 more authors.. (2014) The C-terminal transmembrane domain of human phospholipid scramblase 1 is essential for the protein flip-flop activity and Ca²⁺-binding.. The Journal of membrane biology, [PMID:24343571] |
| 22. Rayala, Sarika S, Francis, Vincent G VG, Sivagnanam, Ulaganathan U and Gummadi, Sathyanarayana N SN.. (2014) N-terminal proline-rich domain is required for scrambling activity of human phospholipid scramblases.. The Journal of biological chemistry, (9): [PMID:24648509] |
| 23. Sivagnanam, Ulaganathan U, Narayana Murthy, Shweta S and Gummadi, Sathyanarayana N SN.. (2016) Identification and characterization of the novel nuclease activity of human phospholipid scramblase 1.. BMC biochemistry, (20): [PMID:27206388] |
| 24. Kusano, Shuichi and Ikeda, Masanori.. (2019) Interaction of phospholipid scramblase 1 with the Epstein-Barr virus protein BZLF1 represses BZLF1-mediated lytic gene transcription.. The Journal of biological chemistry, (11): [PMID:31434743] |
| 25. Guo, Jizheng and 9 more authors.. (2020) Transient Receptor Potential Canonical 5-Scramblase Signaling Complex Mediates Neuronal Phosphatidylserine Externalization and Apoptosis.. Cells, (26): [PMID:32110987] |
