| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab126844-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $66.90 | |
| Ab126844-50μl | 50μl | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $199.90 | |
| Ab126844-100μl | 100μl | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $299.90 | |
| Ab126844-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,299.90 |
mAb (C2); Mouse anti Human SDHA Antibody; WB, ICC, IF; Unconjugated
| Product Name | SDHA Mouse mAb - Primary antibody, specific to SDHA, Mouse IgG2a |
|---|---|
| Synonyms | CMD1GG antibody | DHSA_HUMAN antibody | Flavoprotein subunit of complex II antibody | Fp antibody | PGL5 antibody | SDH 2 antibody | SDH1 antibody | SDH2 antibody | SDHA antibody | SDHF antibody | Succinate dehydrogenase [ubiquinone] flavoprotein subunit |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, Azide Free, High performance, Lot by Lot |
| Host species | Mouse |
| Specificity | SDHA |
| Immunogen | Recombinant SDHA expressed in E.coli (AA 4-300) |
| Positive Control | WB: Hep G2, HeLa, MCF7, A549, LNCaP, HEK293, NIH/3T3 and C6 cell lysates. ICC/IF: HeLa cells. |
| Conjugation | Unconjugated |
| Grade | Azide Free, Carrier Free, ExactAb™, High Performance, Validated |
| Note | 20μl卖完停产，不再备货 |
| Product Description | Mouse anti Human SDHA Antibody, Monoclonal (C2), could be used for WB, ICC, IF and so on. |
| Isotype | Mouse IgG2a |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A and Protein G purified |
| Form | Liquid |
| Concentration | Lot by Lot |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
SDHA Mouse mAb (Ab126844) - Western Blot
All lanes: SDHA Mouse mAb (Ab126844) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution
Predicted band size: 70 kDa
Observed band size: 66 kDa
Exposure time: 2.5 s
SDHA Mouse mAb (Ab126844) - IF/ICC
FITC staining on IF
Samples: Human HeLa cells
Primary Ab: 30 µg/mL SDHA Mouse mAb (Ab126844)
Second Ab: 2 µg/mL FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
| Application | Dilution info |
|---|---|
| WB |
0.01-2 µg/mL
|
| IF/ICC |
5-30 µg/mL
|
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0505914 | Certificate of Analysis | May 21, 2024 | Ab126844 |
| ZJ23F0901139 | Certificate of Analysis | Sep 24, 2023 | Ab126844 |
| ZJ23F0901138 | Certificate of Analysis | Sep 24, 2023 | Ab126844 |
| 1. Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M. (2009) Lysine acetylation targets protein complexes and co-regulates major cellular functions.. Science, 325 (5942): (834-40). [PMID:19608861] [10.1021/op500134e] |
| 2. G Herma Renkema,Saskia B Wortmann,Roel J Smeets,Hanka Venselaar,Marion Antoine,Gepke Visser,Tawfeg Ben-Omran,Lambert P van den Heuvel,Henri J L M Timmers,Jan A Smeitink,Richard J T Rodenburg. (2014-05-02) SDHA mutations causing a multisystem mitochondrial disease: novel mutations and genetic overlap with hereditary tumors.. European journal of human genetics : EJHG, 23 ((2)): (202-209). [PMID:24781757] |
| 3. Bourgeron, T T and 7 more authors.. (1995) Mutation of a nuclear succinate dehydrogenase gene results in mitochondrial respiratory chain deficiency.. Nature genetics, [PMID:7550341] |
| 4. Hirawake, H H, Wang, H H, Kuramochi, T T, Kojima, S S and Kita, K K.. (1994) Human complex II (succinate-ubiquinone oxidoreductase): cDNA cloning of the flavoprotein (Fp) subunit of liver mitochondria.. Journal of biochemistry, [PMID:7798181] |
| 5. Morris, A A AA, Farnsworth, L L, Ackrell, B A BA, Turnbull, D M DM and Birch-Machin, M A MA.. (1994) The cDNA sequence of the flavoprotein subunit of human heart succinate dehydrogenase.. Biochimica et biophysica acta, (29): [PMID:8142412] |
| 6. Parfait, B B and 5 more authors.. (2000) Compound heterozygous mutations in the flavoprotein gene of the respiratory chain complex II in a patient with Leigh syndrome.. Human genetics, [PMID:10746566] |
| 7. Birch-Machin, M A MA, Taylor, R W RW, Cochran, B B, Ackrell, B A BA and Turnbull, D M DM.. (2000) Late-onset optic atrophy, ataxia, and myopathy associated with a mutation of a complex II gene.. Annals of neurology, [PMID:10976639] |
| 8. Van Coster, Rudy R and 9 more authors.. (2003) Homozygous Gly555Glu mutation in the nuclear-encoded 70 kDa flavoprotein gene causes instability of the respiratory chain complex II.. American journal of medical genetics. Part A, (1): [PMID:12794685] |
| 9. Aboulaich, Nabila N, Vainonen, Julia P JP, Strålfors, Peter P and Vener, Alexander V AV.. (2004) Vectorial proteomics reveal targeting, phosphorylation and specific fragmentation of polymerase I and transcript release factor (PTRF) at the surface of caveolae in human adipocytes.. The Biochemical journal, (15): [PMID:15242332] |
| 10. Schmutz, Jeremy J and 75 more authors.. (2004) The DNA sequence and comparative analysis of human chromosome 5.. Nature, (16): [PMID:15372022] |
| 11. Hao, Huai-Xiang HX and 13 more authors.. (2009) SDH5, a gene required for flavination of succinate dehydrogenase, is mutated in paraganglioma.. Science (New York, N.Y.), (28): [PMID:19628817] |
| 12. Burnichon, Nelly N and 13 more authors.. (2010) SDHA is a tumor suppressor gene causing paraganglioma.. Human molecular genetics, (1): [PMID:20484225] |
| 13. Levitas, Aviva A and 8 more authors.. (2010) Familial neonatal isolated cardiomyopathy caused by a mutation in the flavoprotein subunit of succinate dehydrogenase.. European journal of human genetics : EJHG, [PMID:20551992] |
| 14. Ogura, Masato; Yamaki, Junko; Homma, Miwako K and Homma, Yoshimi.. (2012) Mitochondrial c-Src regulates cell survival through phosphorylation of respiratory chain components.. The Biochemical journal, (15): [PMID:22823520] |
| 15. Alston, Charlotte L CL and 12 more authors.. (2012) Recessive germline SDHA and SDHB mutations causing leukodystrophy and isolated mitochondrial complex II deficiency.. Journal of medical genetics, [PMID:22972948] |
| 16. Sciacovelli, Marco M and 12 more authors.. (2013) The mitochondrial chaperone TRAP1 promotes neoplastic growth by inhibiting succinate dehydrogenase.. Cell metabolism, (4): [PMID:23747254] |
| 17. Helman, Guy G and 13 more authors.. (2016) Magnetic resonance imaging spectrum of succinate dehydrogenase-related infantile leukoencephalopathy.. Annals of neurology, [PMID:26642834] |
| 18. Courage, Carolina C and 6 more authors.. (2017) SDHA mutation with dominant transmission results in complex II deficiency with ocular, cardiac, and neurologic involvement.. American journal of medical genetics. Part A, [PMID:27683074] |
| 19. Lahiri, Amit A, Hedl, Matija M, Yan, Jie J and Abraham, Clara C.. (2017) Human LACC1 increases innate receptor-induced responses and a LACC1 disease-risk variant modulates these outcomes.. Nature communications, (8): [PMID:28593945] |
