| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab126902-10μl | 10μl | In stock | $69.90 | |
| Ab126902-50μl | 50μl | In stock | $199.90 | |
| Ab126902-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $319.90 | |
| Ab126902-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,309.90 |
pAb; Rabbit anti Human SEC24B Antibody; WB, Flow; Unconjugated
| Product Name | SEC24B Antibody - Primary antibody, specific to SEC24B, Rabbit IgG |
|---|---|
| Synonyms | SEC24-related protein B | Protein transport protein Sec24B antibody | SC24B_HUMAN antibody | SEC24 antibody | SEC24 family, member B (S. cerevisiae) antibody | SEC24 related gene family, member B antibody | SEC24-related protein B antibody | SEC24B antibo |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, 0.5 mg/mL |
| Host species | Rabbit |
| Specificity | SEC24B |
| Immunogen | A KLH conjugated synthetic peptide from the human region of human SEC24B (AA 1209-1239). |
| Positive Control | WB: 293 lysates. Flow: A431 cells. |
| Conjugation | Unconjugated |
| Grade | Carrier Free, ExactAb™, Validated |
| Product Description | Rabbit anti Human SEC24B Antibody, Polyclonal, could be used for WB, Flow and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified + Peptide affinity purified |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4℃ short term (2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
SEC24B Antibody (Ab126902) - Western Blot
All lanes: SEC24B Antibody (Ab126902) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution
Predicted band size: 137 kDa
Observed band size: 154 kDa
Exposure time: 30.0 s
SEC24B Antibody (Ab126902) - Flow Cytometry
Overlay histogram showing A431 cells stained with SEC24B Antibody (Ab126902) (green line). The cells were fixed with 2% paraformaldehyde and then permeabilized with 90% methanol for 10 min. The cells were then incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by SEC24B Antibody (Ab126902) (1/25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at room temperature. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10000 events was performed.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| Flow Cytometry | 1/25 |
| WB | 1/2000 "> 1/2000 |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0404344 | Certificate of Analysis | Apr 10, 2024 | Ab126902 |
| ZJ24F0404345 | Certificate of Analysis | Apr 10, 2024 | Ab126902 |
| 1. Sharon Gauci,Andreas O Helbig,Monique Slijper,Jeroen Krijgsveld,Albert J R Heck,Shabaz Mohammed. (2009-05-06) Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.. Analytical chemistry, 81 ((11)): (4493-4501). [PMID:19413330] |
| 2. Pagano, A A and 5 more authors.. (1999) Sec24 proteins and sorting at the endoplasmic reticulum.. The Journal of biological chemistry, (19): [PMID:10075675] |
| 3. Hillier, Ladeana W LW and 121 more authors.. (2005) Generation and annotation of the DNA sequences of human chromosomes 2 and 4.. Nature, (7): [PMID:15815621] |
| 4. Mancias, Joseph D JD and Goldberg, Jonathan J.. (2007) The transport signal on Sec22 for packaging into COPII-coated vesicles is a conformational epitope.. Molecular cell, (11): [PMID:17499046] |
| 5. Mancias, Joseph D JD and Goldberg, Jonathan J.. (2008) Structural basis of cargo membrane protein discrimination by the human COPII coat machinery.. The EMBO journal, (5): [PMID:18843296] |
| 6. Mayya, Viveka V and 7 more authors.. (2009) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.. Science signaling, (18): [PMID:19690332] |
| 7. Irisawa, Masato M, Inoue, Jun J, Ozawa, Nozomi N, Mori, Kazutoshi K and Sato, Ryuichiro R.. (2009) The sterol-sensing endoplasmic reticulum (ER) membrane protein TRC8 hampers ER to Golgi transport of sterol regulatory element-binding protein-2 (SREBP-2)/SREBP cleavage-activated protein and reduces SREBP-2 cleavage.. The Journal of biological chemistry, (16): [PMID:19706601] |
| 8. Bonnon, Carine C, Wendeler, Markus W MW, Paccaud, Jean-Pierre JP and Hauri, Hans-Peter HP.. (2010) Selective export of human GPI-anchored proteins from the endoplasmic reticulum.. Journal of cell science, (15): [PMID:20427317] |
| 9. Sauvageau, Etienne E and 7 more authors.. (2014) CNIH4 interacts with newly synthesized GPCR and controls their export from the endoplasmic reticulum.. Traffic (Copenhagen, Denmark), [PMID:24405750] |
