| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab127151-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $79.90 | |
| Ab127151-50μl | 50μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $229.90 | |
| Ab127151-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $359.90 | |
| Ab127151-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $1,999.90 |
pAb; Rabbit anti Human SERPINB1/PI2 Antibody; WB, IHC, ICC, IF; Unconjugated
| Product Name | SERPINB1/PI2 Antibody - Primary antibody, specific to SERPINB1/PI2, Rabbit IgG |
|---|---|
| Synonyms | Anti elastaseantibody | EI antibody | ELANH2antibody | Epididymis luminal protein 57 antibody | HEL57 antibody | ILEU_HUMAN antibody | LEI antibody | Leukocyte elastase inhibitor antibody | M/NEI antibody | MNEIantibody | Monocyte/neutrophil elastase inhi |
| Specifications & Purity | Validated, Carrier Free, ExactAb™, 1.0 mg/mL |
| Biochemical and Physiological Mechanisms | Protein Function： Regulates the activity of the neutrophil proteases elastase, cathepsin G, proteinase-3, chymase, chymotrypsin, and kallikrein-3. |
| Host species | Rabbit |
| Specificity | SERPINB1/PI2 |
| Immunogen | Recombinant full protein of human SERPINB1/PI2. |
| Positive Control | WB: Mouse spleen, THP-1 and U937 lysates. IHC: Human liver cancer tissue. ICC/IF: HeLa cells. |
| Conjugation | Unconjugated |
| Grade | Carrier Free, ExactAb™, Validated |
| Product Description | Rabbit anti Human SERPINB1/PI2 Antibody, Polyclonal, could be used for WB, IHC, ICC, IF and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Immunogen affinity purified |
| Form | Liquid |
| Concentration | 1.0 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
SERPINB1/PI2 Antibody (Ab127151) - Western Blot
All lanes: SERPINB1/PI2 Antibody (Ab127151) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution
Predicted band size: 42 kDa
Observed band size: 37, 41 kDa
Exposure time: 7.3 s
SERPINB1/PI2 Antibody (Ab127151) - Western Blot
All lanes: SERPINB1/PI2 Antibody (Ab127151) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/40000 dilution
Predicted band size: 42 kDa
Observed band size: 41 kDa
Exposure time: 26.3 s
SERPINB1/PI2 Antibody (Ab127151) - IHC
Immunohistochemical analysis of SerpinB1 staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with SERPINB1/PI2 Antibody (Ab127151) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
SERPINB1/PI2 Antibody (Ab127151) - IF/ICC
Immunofluorescent analysis of SERPINB1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with SERPINB1/PI2 Antibody (Ab127151) in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/2000 "> 1/500-1/2000 |
| IF/ICC |
1/50-1/200
">
1/50-1/200
|
| IHC | 1/50 - 1/100 "> 1/50 - 1/100 |
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