mAb (5#); Mouse anti Human SFRP4 Antibody; WB,IHC; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab127304-20μl | 20μl | In stock | $109.90 | |
| Ab127304-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $76.90 | |
| Ab127304-50μl | 50μl | In stock | $219.90 | |
| Ab127304-100μl | 100μl | In stock | $369.90 | |
| Ab127304-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,489.90 |
mAb (5#); Mouse anti Human SFRP4 Antibody; WB,IHC; Unconjugated
| Product Name | SFRP4 Mouse mAb - Primary antibody, specific to SFRP4, Mouse IgG2b |
|---|---|
| Synonyms | DDC-4 | Frizzled protein, human endometrium | FRP-4 | FRP-AP | frpHE | FRPHEMGC26498 | FrzB2 | secreted frizzled-related protein 4 | sFRP4 | sFRP-4 | Frizzled protein antibody | Frizzled protein human endometrium antibody | FRP 4 antibody | FRP4 antibody |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, Azide Free, Lot by Lot |
| Host species | Mouse |
| Specificity | SFRP4 |
| Immunogen | Recombinant SFRP4 expressed in E.coli (AA 265-346) |
| Positive Control | WB: HEK293, HeLa, MCF7 and C6 cell lysates. IHC: Human colon tissue. |
| Conjugation | Unconjugated |
| Grade | Azide Free, Carrier Free, ExactAb™, Validated |
| Product Description | Mouse anti Human SFRP4 Antibody, Monoclonal (5#), could be used for WB, IHC and so on. Application: WB: 0.5-2µg/mL IHC：5-20µg/mL Protein Function Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP4 may act as a regulator of adult uterine morphology and function. Increases apoptosis during ovulation possibly through modulation of FZ1/FZ4/WNT4 signaling (By similarity). Has phosphaturic effects by specifically inhibiting sodium-dependent phosphate uptake. |
| Isotype | Mouse IgG2b |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A + Protein G purified |
| Form | Liquid |
| Concentration | Lot by Lot |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
SFRP4 Mouse mAb (Ab127304) - Western Blot
All lanes: SFRP4 Mouse mAb (Ab127304) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution
Predicted band size: 40 kDa
Observed band size: 36 kDa
Exposure time: 150.0 s
SFRP4 Mouse mAb (Ab127304) - IHC
DAB staining on IHC
Sample: Human Colon Tissue
Primary Ab: 20 µg/mL SFRP4 Mouse mAb (Ab127304)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| IHC | 5-20 µg/mL |
| WB | 0.5-2µg/mL |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F1201995 | Certificate of Analysis | Dec 13, 2023 | Ab127304 |
| ZJ23F1201994 | Certificate of Analysis | Dec 13, 2023 | Ab127304 |
| ZJ23F1201993 | Certificate of Analysis | Dec 13, 2023 | Ab127304 |
| 1. Scherer SW, Cheung J, MacDonald JR, Osborne LR, Nakabayashi K, Herbrick JA, Carson AR, Parker-Katiraee L, Skaug J, Khaja R et al.. (2003) Human chromosome 7: DNA sequence and biology.. Science, 300 (5620): (767-72). [PMID:12690205] |
| 2. Abu-Jawdeh, G G and 6 more authors.. (1999) Differential expression of frpHE: a novel human stromal protein of the secreted frizzled gene family, during the endometrial cycle and malignancy.. Laboratory investigation; a journal of technical methods and pathology, [PMID:10211996] |
| 3. Schumann, H H, Holtz, J J, Zerkowski, H R HR and Hatzfeld, M M.. (2000) Expression of secreted frizzled related proteins 3 and 4 in human ventricular myocardium correlates with apoptosis related gene expression.. Cardiovascular research, [PMID:10728394] |
| 4. Berndt, Theresa T and 8 more authors.. (2003) Secreted frizzled-related protein 4 is a potent tumor-derived phosphaturic agent.. The Journal of clinical investigation, [PMID:12952927] |
| 5. Drake, J J and 6 more authors.. (2009) Expression of secreted frizzled-related protein 4 (SFRP4) in primary serous ovarian tumours.. European journal of gynaecological oncology, [PMID:19480240] |
| 6. Bukhari, Shazia Anwer SA, Shamshari, Waseem Akhtar WA, Ur-Rahman, Mahmood M, Zia-Ul-Haq, Muhammad M and Jaafar, Hawa Z E HZ.. (2014) Computer aided screening of secreted frizzled-related protein 4 (SFRP4): a potential control for diabetes mellitus.. Molecules (Basel, Switzerland), (11): [PMID:25019556] |
| 7. Kiper, Pelin O Simsek POS and 23 more authors.. (2016) Cortical-Bone Fragility--Insights from sFRP4 Deficiency in Pyle's Disease.. The New England journal of medicine, (30): [PMID:27355534] |
