| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab128237-10μl | 10μl | In stock | $66.90 | |
| Ab128237-50μl | 50μl | In stock | $189.90 | |
| Ab128237-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $299.90 | |
| Ab128237-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,699.90 |
mAb (4B10-B10-B6); Mouse anti Human SMAD5 Antibody; WB, IF, ICC, Flow; Unconjugated
| Product Name | SMAD5 Mouse mAb - Primary antibody, specific to SMAD5, Mouse IgG1 |
|---|---|
| Synonyms | Dwfc | hSmad5 | JV5-1 | JV5-1DKFZp781O1323 | MAD homolog 5 | MAD, mothers against decapentaplegic homolog 5 (Drosophila) | MADH5 | MADH5mothers against decapentaplegic homolog 5 | mothers against decapentaplegic homolog 5 | mothers against decapentaplegic |
| Specifications & Purity | ExactAb™, Validated, 1.6 mg/mL |
| Host species | Mouse |
| Specificity | SMAD5 |
| Immunogen | Purified recombinant human SMAD5 (C-terminus) protein fragments expressed in E.coli. (AA 1-465). |
| Positive Control | WB: HeLa, SK-N-SH, NIH/3T3 and C6 cell lysates. IF/ICC: HeLa cells. Flow: Jurkat cells. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Validated |
| Product Description |
Mouse anti Human SMAD5 Antibody, Monoclonal (4B10-B10-B6), could be used for WB, IF/ICC, Flow and so on. |
| Isotype | Mouse IgG1 |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein G purified |
| Form | Liquid |
| Concentration | 1.6 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant SMAD5 Antibody (Ab128237) - Western Blot
All lanes: Recombinant SMAD5 Antibody (Ab128237) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 52 kDa
Observed band size: 50 kDa
Exposure time: 1.0 s
Recombinant SMAD5 Antibody (Ab128237) - IF/ICC
Immunocytochemistry analysis of Smad5 in HeLa cells using Recombinant SMAD5 Antibody (Ab128237).
Recombinant SMAD5 Antibody (Ab128237) - Flow Cytometry
Flow Cytometry analysis of Jurkat cells stained with Recombinant SMAD5 Antibody (Ab128237) (red). Black line histogram represents the isotype control, normal mouse IgG.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000"> 1/500-1/1000 |
| IF/ICC | 1/50 - 1/200 "> 1/50 - 1/200 |
| Flow Cytometry |
1/50-1/100
|
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0404708 | Certificate of Analysis | Apr 22, 2024 | Ab128237 |
| ZJ24F0404707 | Certificate of Analysis | Apr 22, 2024 | Ab128237 |
| 1. Sharon Gauci,Andreas O Helbig,Monique Slijper,Jeroen Krijgsveld,Albert J R Heck,Shabaz Mohammed. (2009-05-06) Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.. Analytical chemistry, 81 ((11)): (4493-4501). [PMID:19413330] |
| 2. Riggins, G J GJ and 9 more authors.. (1996) Mad-related genes in the human.. Nature genetics, [PMID:8673135] |
| 3. Zavadil, J J, Brezinová, J J, Svoboda, P P, Zemanová, Z Z and Michalová, K K.. (1997) Smad5, a tumor suppressor candidate at 5q31.1, is hemizygously lost and not mutated in the retained allele in human leukemia cell line HL60.. Leukemia, [PMID:9264367] |
| 4. Hejlik, D P DP and 10 more authors.. (1997) Localization of SMAD5 and its evaluation as a candidate myeloid tumor suppressor.. Cancer research, (1): [PMID:9288787] |
| 5. Nishimura, R R and 5 more authors.. (1998) Smad5 and DPC4 are key molecules in mediating BMP-2-induced osteoblastic differentiation of the pluripotent mesenchymal precursor cell line C2C12.. The Journal of biological chemistry, (23): [PMID:9442019] |
| 6. Gemma, A A and 7 more authors.. (1998) hSmad5 gene, a human hSmad family member: its full length cDNA, genomic structure, promoter region and mutation analysis in human tumors.. Oncogene, (19): [PMID:9484787] |
| 7. Massagué, J J.. (1998) TGF-beta signal transduction.. Annual review of biochemistry, [PMID:9759503] |
| 8. Verschueren, K K and Huylebroeck, D D.. () Remarkable versatility of Smad proteins in the nucleus of transforming growth factor-beta activated cells.. Cytokine & growth factor reviews, [PMID:10647776] |
| 9. Wrana, J L JL and Attisano, L L.. () The Smad pathway.. Cytokine & growth factor reviews, [PMID:10708948] |
| 10. Miyazono, K K.. () TGF-beta signaling by Smad proteins.. Cytokine & growth factor reviews, [PMID:10708949] |
| 11. Jiao, Kai K, Zhou, Yingna Y and Hogan, Brigid L M BL.. (2002) Identification of mZnf8, a mouse Krüppel-like transcriptional repressor, as a novel nuclear interaction partner of Smad1.. Molecular and cellular biology, [PMID:12370310] |
| 12. Frontelo, Pilar P and 6 more authors.. (2004) Suv39h histone methyltransferases interact with Smads and cooperate in BMP-induced repression.. Oncogene, (1): [PMID:15107829] |
| 13. Pan, Deng D and 6 more authors.. (2005) The integral inner nuclear membrane protein MAN1 physically interacts with the R-Smad proteins to repress signaling by the transforming growth factor-{beta} superfamily of cytokines.. The Journal of biological chemistry, (22): [PMID:15647271] |
| 14. Bienvenut, Willy V WV and 6 more authors.. (2012) Comparative large scale characterization of plant versus mammal proteins reveals similar and idiosyncratic N-α-acetylation features.. Molecular & cellular proteomics : MCP, [PMID:22223895] |
| 15. Van Damme, Petra P and 15 more authors.. (2012) N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB.. Proceedings of the National Academy of Sciences of the United States of America, (31): [PMID:22814378] |
| 16. Chen, Fenfang F and 9 more authors.. (2015) Nuclear Export of Smads by RanBP3L Regulates Bone Morphogenetic Protein Signaling and Mesenchymal Stem Cell Differentiation.. Molecular and cellular biology, [PMID:25755279] |
