SOD2/MnSOD Antibody - Primary antibody, specific to SOD2

Features and benefits
  • Short Overview:

    pAb; Rabbit anti Human SOD2/MnSOD Antibody; WB, IHC ; Unconjugated

  • Species reactivity(Reacts with): Human,Mouse
    Application:
  • IHC
  • WB
Item Number
Ab128615
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab128615-10μl
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$66.90
Ab128615-20μl
20μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$100.90
Ab128615-50μl
50μl
In stock
$189.90
Ab128615-100μl
100μl
In stock
$299.90
Ab128615-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,019.90

pAb; Rabbit anti Human SOD2/MnSOD Antibody; WB, IHC ; Unconjugated

Basic Description

Product NameSOD2/MnSOD Antibody - Primary antibody, specific to SOD2
SynonymsEC 1.15.1.1; indophenoloxidase B; IPOB; IPO-B; manganese-containing superoxide dismutase; mangano-superoxide dismutase; Mn SOD; Mn superoxide dismutase; MnSOD; MVCD6; SOD, Mitochodrial; SOD2; superoxide dismutase [Mn], mitochondrial; superoxide dismutase
Specifications & PurityExactAb™, Validated, Carrier Free, Azide Free, Lot by Lot
Host speciesRabbit
SpecificitySOD2
ImmunogenRecombinant SOD2 expressed in E.coli (AA 25-222).
Positive ControlWB: HeLa, MCF7, HEK293, A431, A549, Hep G2, K562 and NIH/3T3 cell lysates. IHC: Human prostate cancer, breast cancer, kidney, skin cancer, colorectal cancer, liver tissues.
ConjugationUnconjugated
GradeAzide Free, Carrier Free, ExactAb™, Validated
Product Description

Rabbit anti Human SOD2/MnSOD Antibody, Polyclonal, could be used for WB, IHC and so on.

Application

WB: 0.5-3µg/mL IHC: 5-20µg/mL

Protein Function

Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems.

Product Properties

SDS-PAGE150 kDa
Purification MethodAntigen affinity purified + Protein A purified
FormLiquid
ConcentrationLot by Lot
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

SOD2/MnSOD Antibody (Ab128615) - Western Blot
All lanes: SOD2/MnSOD Antibody (Ab128615) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 24 kDa
Observed band size: 24 kDa
Exposure time: 1.3 s

SOD2/MnSOD Antibody (Ab128615) - IHC
DAB staining on IHC
Samples: Human Prostate cancer Tissue
Primary Ab: 10 µg/mL SOD2/MnSOD Antibody (Ab128615)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

SOD2/MnSOD Antibody (Ab128615) - IHC
DAB staining on IHC
Samples: Human Breast cancer Tissue
Primary Ab: 10 µg/mL SOD2/MnSOD Antibody (Ab128615)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

SOD2/MnSOD Antibody (Ab128615) - IHC
DAB staining on IHC
Samples: Human Kidney Tissue
Primary Ab: 10 µg/mL SOD2/MnSOD Antibody (Ab128615)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

SOD2/MnSOD Antibody (Ab128615) - IHC
DAB staining on IHC
Samples: Human Skin cancer Tissue
Primary Ab: 10 µg/mL SOD2/MnSOD Antibody (Ab128615)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

SOD2/MnSOD Antibody (Ab128615) - IHC
DAB staining on IHC
Samples: Human Colorectal cancer Tissue
Primary Ab: 10 µg/mL SOD2/MnSOD Antibody (Ab128615)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

SOD2/MnSOD Antibody (Ab128615) - IHC
DAB staining on IHC
Samples: Human Liver Tissue
Primary Ab: 10 µg/mL SOD2/MnSOD Antibody (Ab128615)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

Associated Targets(Human)

SOD2 Tbio Superoxide dismutase [Mn], mitochondrial (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
SOD2 Tbio Superoxide dismutase [Mn], mitochondrial (6 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID

Application

ApplicationDilution info
IHC

5-20 µg/mL

WB

0.5-3 µg/mL

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot NumberCertificate TypeDateItem
ZJ23F1101549Certificate of AnalysisNov 02, 2023 Ab128615
ZJ23F1101550Certificate of AnalysisNov 02, 2023 Ab128615

Related Documents

References

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2. Borgstahl, G E GE and 5 more authors..  (1992)  The structure of human mitochondrial manganese superoxide dismutase reveals a novel tetrameric interface of two 4-helix bundles..  Cell,    (2):   [PMID:1394426]
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11. Wan, X S XS, Devalaraja, M N MN and St Clair, D K DK..  (1994)  Molecular structure and organization of the human manganese superoxide dismutase gene..  DNA and cell biology,      [PMID:7702755]
12. Corbett, J M JM, Wheeler, C H CH, Baker, C S CS, Yacoub, M H MH and Dunn, M J MJ..  (1994)  The human myocardial two-dimensional gel protein database: update 1994..  Electrophoresis,      [PMID:7895732]
13. Borgstahl, G E GE and 8 more authors..  (1996)  Human mitochondrial manganese superoxide dismutase polymorphic variant Ile58Thr reduces activity by destabilizing the tetrameric interface..  Biochemistry,    (9):   [PMID:8605177]
14. Rasmussen, R K RK and 6 more authors..  ()  Two-dimensional electrophoretic analysis of human breast carcinoma proteins: mapping of proteins that bind to the SH3 domain of mixed lineage kinase MLK2..  Electrophoresis,      [PMID:9150946]
15. Guan, Y Y and 9 more authors..  (1998)  Crystal structure of Y34F mutant human mitochondrial manganese superoxide dismutase and the functional role of tyrosine 34..  Biochemistry,    (7):   [PMID:9537987]
16. Hsieh, Y Y and 9 more authors..  (1998)  Probing the active site of human manganese superoxide dismutase: the role of glutamine 143..  Biochemistry,    (7):   [PMID:9537988]
17. Zhao, J J, Lahiri-Chatterjee, M M, Sharma, Y Y and Agarwal, R R..  (2000)  Inhibitory effect of a flavonoid antioxidant silymarin on benzoyl peroxide-induced tumor promotion, oxidative stress and inflammatory responses in SENCAR mouse skin..  Carcinogenesis,      [PMID:10753220]
18. Lévêque, V J VJ and 6 more authors..  (2000)  Multiple replacements of glutamine 143 in human manganese superoxide dismutase: effects on structure, stability, and catalysis..  Biochemistry,    (20):   [PMID:10852710]
19. Hearn, A S AS and 6 more authors..  (2001)  Kinetic analysis of product inhibition in human manganese superoxide dismutase..  Biochemistry,    (9):   [PMID:11580280]
20. Nomiyama, Takashi T and 8 more authors..  (2003)  The polymorphism of manganese superoxide dismutase is associated with diabetic nephropathy in Japanese type 2 diabetic patients..  Journal of human genetics,      [PMID:12624725]
21. Xu, Shanqin S and 11 more authors..  (2006)  Detection of sequence-specific tyrosine nitration of manganese SOD and SERCA in cardiovascular disease and aging..  American journal of physiology. Heart and circulatory physiology,      [PMID:16399855]
22. Liu, Limei L and 10 more authors..  (2009)  The manganese superoxide dismutase Val16Ala polymorphism is associated with decreased risk of diabetic nephropathy in Chinese patients with type 2 diabetes..  Molecular and cellular biochemistry,      [PMID:18989629]
23. Perry, J Jefferson P JJ and 5 more authors..  (2009)  Contribution of human manganese superoxide dismutase tyrosine 34 to structure and catalysis..  Biochemistry,    (21):   [PMID:19265433]
24. Goitre, Luca L and 6 more authors..  (2010)  KRIT1 regulates the homeostasis of intracellular reactive oxygen species..  PloS one,    (26):   [PMID:20668652]
25. Kim, Myung-Sun MS, Ramakrishna, Suresh S, Lim, Key-Hwan KH, Kim, Jun-Hyun JH and Baek, Kwang-Hyun KH..  (2011)  Protein stability of mitochondrial superoxide dismutase SOD2 is regulated by USP36..  Journal of cellular biochemistry,      [PMID:21268071]

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