Src (phospho Y529) Antibody - Primary antibody, specific to Src (phospho Y529), Rabbit IgG
Synonyms
Src (phospho Y529) | p-Src (phospho Y529) | Src(Phospho-Tyr529) | ASV | Avian sarcoma virus | c SRC | CDNA FLJ14219 fis clone NT2RP3003800 highly similar to Rattus norvegicus tyrosine protein kinase pp60 c src mRNA | cSrc | EC 2.7.10.2 | Neuronal CSRC tyr
Specifications & Purity
Validated, 1.0 mg/mL
Host species
Rabbit
Specificity
Src (phospho Y529)
Immunogen
A synthetic peptide derived from human Src (phospho Y529) (AA 487-536).
Positive Control
WB: COS7, 3T3 lysates. IHC: Human breast carcinoma tissue
Conjugation
Unconjugated
Grade
Validated
Product Description
Rabbit polyclonal to Src (phospho Y529), Polyclonal, could be used for WB, IHC and so on. Applications: WB: 1/500 - 1/2000 IHC: 1/100 - 1/300 Protein Function Non-receptor protein tyrosine kinase that plays pivotal roles in numerous cellular processes such as proliferation, migration, and transformation. In concert with PTK2B, plays an important role in osteoclastic bone resorption. Both the formation of a SRC-PTK2B complex, and SRC kinase activity are necessary for this function. Once it is recruited to the activated integrins, by PTK2B, it phosphorylates CBL which in turn induces the activation and recruitment of phosphatidylinositol 3-kinase to the cell membrane in a signaling pathway that is critical for osteoclast function. Promotes energy production in osteoclasts by activating mitochondrial cytochrome C oxidase. Phosphorylates RUNX3 and COX2 on tyrosine residues, TNK2 on 'Tyr-284' and CBL on 'Tyr-731'. Enhances DDX58/RIG-I-elicited antiviral signaling.
Product Properties
Isotype
Rabbit IgG
SDS-PAGE
150kDa
Purification Method
Immunogen affinity purified
Form
Liquid
Concentration
1.0 mg/mL
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (12 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
Src (phospho Y529) Antibody (Ab129131) - Western Blot All lanes: Src (phospho Y529) Antibody (Ab129131) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 60 kDa Observed band size: 58 kDa Exposure time: 3.0 s
Src (phospho Y529) Antibody (Ab129131) - Western Blot All lanes: Src (phospho Y529) Antibody (Ab129131) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 60 kDa Observed band size: 58 kDa Exposure time: 5.0 s
Src (phospho Y529) Antibody (Ab129131) - IHC Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Src (phospho Y529) Antibody (Ab129131). The picture on the right is blocked with the phospho peptide.
Src (phospho Y529) Antibody (Ab129131) - ELISA Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Src (phospho Y529) Antibody (Ab129131).