SUPT4H Antibody

    Application:
  • WB
Features and benefits
  • Host species: Rabbit
  • Species reactivity(Reacts with): Human,Mouse,Rat
  • Isotype: Rabbit IgG
  • Conjugation: Unconjugated
Item Number
Ab129809
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab129809-10μl
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$69.90
Ab129809-50μl
50μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$189.90
Ab129809-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$299.90
Ab129809-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$1,999.90

pAb; Rabbit anti Human SUPT4H Antibody; WB; Unconjugated

Basic Description

Product NameSUPT4H Antibody
SynonymsDRB sensitivity-inducing factor small subunit antibody | DSIF p14 antibody | DSIF small subunit antibody | hSPT4 antibody | SPT4 antibody | SPT4H antibody | SPT4H_HUMAN antibody | SUPT4H `1 antibody | SUPT4H antibody | Supt4h1 antibody | Transcription elo
Specifications & PurityExactAb™, Validated, 1.0 mg/mL
Host speciesRabbit
SpecificitySUPT4H1
ImmunogenA synthetic peptide derived from human SUPT4H (AA 40-120)
Positive ControlWB: K562, MCF7, HEK293 and Hep G2 cell lysates.
ConjugationUnconjugated
GradeExactAb™, Validated
Product Description

Rabbit anti Human SUPT4H Antibody, Polyclonal, could be used for WB and so on.
Application:
WB: 1/500-1/2000
Protein Function
Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. TFIIS/S-II binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. Cleavage of blocked transcripts by RNA polymerase II promotes the resumption of transcription from the new 3' terminus and may allow repeated attempts at transcription through natural pause sites. DSIF can also positively regulate transcriptional elongation and is required for the efficient activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat. DSIF acts to suppress transcriptional pausing in transcripts derived from the HIV-1 LTR and blocks premature release of HIV-1 transcripts at terminator sequences.

Product Properties

IsotypeRabbit IgG
SDS-PAGE150 kDa
Purification MethodImmunogen affinity purified
FormLiquid
Concentration1.0 mg/mL
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at 4°C short term (1-2 weeks). Store at -20°C long term (12 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

SUPT4H Antibody (Ab129809) - Western Blot
All lanes: SUPT4H Antibody (Ab129809) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 13 kDa
Observed band size: 12 kDa
Exposure time: 12.6 s

Associated Targets(Human)

SUPT4H1 Tbio Transcription elongation factor SPT4 (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

Application

ApplicationDilution info
WB1/500-1/2000

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1/500-1/2000

Certificates

Certificate of Analysis(COA)

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Related Documents

Solution Calculators