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Thymidine - 99%, high purity , CAS No.50-89-5

19 Citations
Item Number
T104771
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T104771-1g
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T104771-5g
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$292.90
T104771-10g
10g
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$526.90
T104771-25g
25g
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$1,184.90
T104771-100g
100g
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$4,264.90
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500g
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$19,190.90
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Pyrimidine deoxynucleoside. Potent thymidine kinase substrate.

View related series
Anti-infection Cell Cycle/DNA Damage DNA/RNA Synthesis Endogenous Metabolite Metabolic Enzyme/Protease Orthopoxvirus Pyrimidines Six-Membered Heterocyclic Compounds

Basic Description

SynonymsEINECS 200-070-4 | DB04485 | 1-((2R,4S,5R)-4-Hydroxy-5-(hydroxymethyl)tetrahydro-furan-2-yl)-5-methylpyrimidine-2,4(1H,3H)-dione | 1-((2R,4S,5R)-4-Hydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-5-methylpyrimidine-2,4(1H,3H)-dione | SR-01000883981 | EN300-
Specifications & PurityMoligand™, ≥99%
Biochemical and Physiological MechanismsPyrimidine deoxynucleoside. Potent thymidine kinase substrate (IC 50 = 16 nM). Pairs with deoxyadenosine in double-stranded DNA. Synchronizes cells in S phase in vivo.
Storage TempStore at 2-8°C,Argon charged
Shipped InWet ice
GradeMoligand™
NoteWherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour. Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
Product Description

Thymidine is also referred to as pyrimidine deoxynucleoside. Deoxythymidine is a nucleoside present in DNA. In a DNA double stranded structure, thymidine pairs with deoxyadeninosine. It is useful in cell synchronization during S-phase. In the salvage pathway of pyrimidines, pyrimidine phosphorylase reversibly converts thymine to thymidine.
Thymidine (dT) is a pyrimidine deoxyriboside (deoxynucleoside) that upon sequential phosphorylation to deoxythymidine triphospate (dTTP) is used to synthesize DNA and the T-loop of transfer RNA. Thymidine is as substrate of Thymidine kinase EC 2.7.1.21 which phosphorylates the nucleoside with a phosphate derived from ATP. dT may be used to study the distribution, specificity and kinetics of a family of thymidine kinase(s).

Associated Targets(Human)

TK1 Tchem Thymidine kinase, cytosolic (2 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
TYMS Tclin Thymidylate synthase (1651 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
TK1 Tchem Thymidine kinase, cytosolic (627 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
143B (353 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
CCRF-CEM (65223 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
CFPAC-1 (421 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
HL-60 (67320 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
MT4 (17854 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
ATH-8 cell line (300 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
HEL (6614 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
HepG2 (196354 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
HeLa (62764 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
SLC22A1 Tchem Solute carrier family 22 member 1 (646 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
APEX1 Tchem DNA-(apurinic or apyrimidinic site) lyase (38016 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
PMP22 Tbio Peripheral myelin protein 22 (699 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
ATAD2 Tchem ATPase family AAA domain-containing protein 2 (598 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
DCLRE1A Tbio DNA cross-link repair 1A protein (19 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID

Associated Targets(non-human)

TK Thymidine kinase (276 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
tmk Thymidylate kinase (360 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
dnk Deoxynucleoside kinase (95 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
deoA Thymidine phosphorylase (531 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
Enterococcus faecium (13803 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
Mycobacterium tuberculosis (203094 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
Human alphaherpesvirus 2 (4932 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
Artemia (698 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
Mus musculus (284745 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
Human alphaherpesvirus 1 (11089 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
Hepatitis B virus (7925 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
Human immunodeficiency virus 1 (70413 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
Human adenovirus 2 (239 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
Human immunodeficiency virus 2 (5592 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
L1210 (27553 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
Vero (26788 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
MC-38 (857 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
KBALB cell line (25 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
L929 (3802 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
LM (36 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
rep Replicase polyprotein 1ab (378 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID

Mechanisms of Action

Mechanism of ActionAction Typetarget IDTarget NameTarget TypeTarget OrganismBinding Site NameReferences

Names and Identifiers

Pubchem Sid488179935
Pubchem Sid Urlhttps://pubchem.ncbi.nlm.nih.gov/substance/488179935
IUPAC Name 1-[(2R,4S,5R)-4-hydroxy-5-(hydroxymethyl)oxolan-2-yl]-5-methylpyrimidine-2,4-dione
INCHI InChI=1S/C10H14N2O5/c1-5-3-12(10(16)11-9(5)15)8-2-6(14)7(4-13)17-8/h3,6-8,13-14H,2,4H2,1H3,(H,11,15,16)/t6-,7+,8+/m0/s1
InChi Key IQFYYKKMVGJFEH-XLPZGREQSA-N
Canonical SMILES CC1=CN(C(=O)NC1=O)C2CC(C(O2)CO)O
Isomeric SMILES CC1=CN(C(=O)NC1=O)[C@H]2C[C@@H]([C@H](O2)CO)O
WGK Germany 3
RTECS XP2071000
PubChem CID 5789
Molecular Weight 242.23
Beilstein 89285
Reaxy-Rn 89285

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

Find and download the COA for your product by matching the lot number on the packaging.

26 results found

Lot NumberCertificate TypeDateItem
J2424887Certificate of AnalysisJul 01, 2024 T104771
J2424886Certificate of AnalysisJul 01, 2024 T104771
J2424883Certificate of AnalysisJul 01, 2024 T104771
G2326671Certificate of AnalysisJul 03, 2023 T104771
J2425149Certificate of AnalysisJul 03, 2023 T104771
G2326989Certificate of AnalysisJul 03, 2023 T104771
G2326981Certificate of AnalysisJul 03, 2023 T104771
G2326976Certificate of AnalysisJul 03, 2023 T104771
G2326973Certificate of AnalysisJul 03, 2023 T104771
G2326969Certificate of AnalysisJul 03, 2023 T104771
G2326968Certificate of AnalysisJul 03, 2023 T104771
G2326666Certificate of AnalysisJul 03, 2023 T104771
G2326603Certificate of AnalysisJul 03, 2023 T104771
G2326597Certificate of AnalysisJul 03, 2023 T104771
G2326592Certificate of AnalysisJul 03, 2023 T104771
G2326578Certificate of AnalysisJul 03, 2023 T104771
L2208984Certificate of AnalysisNov 19, 2022 T104771
L22081016Certificate of AnalysisNov 19, 2022 T104771
L22081067Certificate of AnalysisNov 19, 2022 T104771
L2208969Certificate of AnalysisNov 19, 2022 T104771
L2208975Certificate of AnalysisNov 19, 2022 T104771
F2201125Certificate of AnalysisMar 19, 2022 T104771
F2201123Certificate of AnalysisMar 19, 2022 T104771
F2201118Certificate of AnalysisMar 19, 2022 T104771
F2201112Certificate of AnalysisMar 19, 2022 T104771
F2201057Certificate of AnalysisMar 19, 2022 T104771

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Chemical and Physical Properties

SolubilitySoluble in water, methanol, hot ethanol, hot acetone, hot ethyl acetate, pyridine, glacial acetic acid and hydrochloric acid. Slightly soluble in hot chloroform .
SensitivityLight sensitive;Hygroscopic
Specific Rotation[α]33 ° (C=1, 1mol/L NaOH)
Melt Point(°C)183-191°C

Safety and Hazards(GHS)

Pictogram(s) GHS08,   GHS07
Signal Danger
Hazard Statements

H335:May cause respiratory irritation

H341:Suspected of causing genetic defects

H302:Harmful if swallowed

H340:May cause genetic defects

Precautionary Statements

P261:Avoid breathing dust/fume/gas/mist/vapors/spray.

P405:Store locked up.

P501:Dispose of contents/container to ...

P264:Wash hands [and …] thoroughly after handling.

P281:Use personal protective equipment as required.

P271:Use only outdoors or in a well-ventilated area.

P270:Do not eat, drink or smoke when using this product.

P304+P340:IF INHALED: Remove person to fresh air and keep comfortable for breathing.

P403+P233:Store in a well-ventilated place. Keep container tightly closed.

P330:Rinse mouth.

P203:Obtain, read and follow all safety instructions before use.

P301+P317:IF SWALLOWED: Get medical help.

P318:if exposed or concerned, get medical advice.

P319:Get medical help if you feel unwell.

WGK Germany 3
RTECS XP2071000
Reaxy-Rn 89285
Merck Index 9397

Related Documents

 SDS

 Specification Sheet

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FAQs and Articles

FAQ
Application of nucleoside pharmaceutical intermediates
Categories: Technical articles
Tags:
Nucleoside Nucleotide

Citations of This Product

How to Cite Aladdin Scientific Products?
1. Xu Lian, Li Hui-Min, Lin Juan.  (2023)  Efficient synthesis of 2’-deoxyguanosine in one-pot cascade by employing an engineered purine nucleoside phosphorylase from Brevibacterium acetylicum.  WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,  39  (10): (1-9).  [PMID:37606812] [10.1007/s11274-023-03721-1]
2. Fei Wang, Fan Yang, Jiawei Liu, Quan Bai.  (2023)  Studies on the retention mechanism of solutes in hydrophilic interaction chromatography using stoichiometric displacement theory II. HILIC/RPLC dual-retention mechanism of solutes in hydrophilic interaction chromatography over the entire range of water concentration in mobile phase.  TALANTA,  265  (124858).  [PMID:37385194] [10.1016/j.talanta.2023.124858]
3. Ye Shu, Tong Chen, Tao Liu, Hechen Xu, Jinjin He, Zhihui Cai, Wanning Zhu, Zihan Zhao, Jun Jiang.  (2023)  Preparation and characterization of mixed-mode hydrophilic stationary phase based on triple hydrophilic functionalized moieties and its application to traditional Chinese herbal compound.  MICROCHEMICAL JOURNAL,  191  (108863).  [PMID:] [10.1016/j.microc.2023.108863]
4. Li Gao, Yuan Wang, Yaqiong Qin, Yaming Sun, Lijun He, Shusheng Zhang, Wenjie Zhao.  (2023)  Preparation and characterization of glucose-based covalent organic polymer coated silica as stationary phase for high-performance liquid chromatography.  JOURNAL OF CHROMATOGRAPHY A,  1693  (463876).  [PMID:36857980] [10.1016/j.chroma.2023.463876]
5. Yan Wu, Kaixing Luo, Yanjuan Liu, Wei Chen, Zhengwu Bai, Sheng Tang.  (2022)  Innovative preparation of ureido/dodecyl dual-functionalized silica as a versatile mixed-mode stationary phase for high-resolution chromatographic separations.  JOURNAL OF CHROMATOGRAPHY A,  1665  (462834).  [PMID:35085896] [10.1016/j.chroma.2022.462834]
6. Jia Ding, Fei Liu, Chunling Qi, Yunlei Zhou, Huanshun Yin, Shiyun Ai.  (2021)  Enhanced photoactivity of perovskite Bi4NbO8Cl/PTC-NH2 heterojunction and its application for photoelectrochemical sensing of DNA hydroxymethylation.  SENSORS AND ACTUATORS B-CHEMICAL,  344  (130211).  [PMID:] [10.1016/j.snb.2021.130211]
7. Qian Wang, Huanshun Yin, Yunlei Zhou, Jun Wang, Shiyun Ai.  (2021)  Photoelectrochemical biosensor for 5-formylcytosine deoxyribonucleoside detection based on BiIO4-WS2/CuO ternary heterojunction.  SENSORS AND ACTUATORS B-CHEMICAL,  341  (130019).  [PMID:] [10.1016/j.snb.2021.130019]
8. Tong Chen, Liang Xu, Guangsan Song, Yuan Li, Hong Xu, Hongbin Zhou, Zhen Xiao, Ping Li.  (2021)  Preparation and application of Au nanoparticles-decorated SO3H-cofunctionalized silica stationary phase for per aqueous liquid chromatography.  MICROCHEMICAL JOURNAL,  164  (105985).  [PMID:] [10.1016/j.microc.2021.105985]
9. Chao Fan, Hao Tang, Licheng Wang, Yijing Li, Xusheng Wang, Shuai Wang, Xiaojing Liang.  (2020)  The preparation of a core–shell stationary phase by the in situ polymerization of a hydrophilic polymer on the surface of silica and its chromatographic performance.  NEW JOURNAL OF CHEMISTRY,  44  (27): (11704-11709).  [PMID:] [10.1039/D0NJ01197E]
10. Zhonghua Dong, Yueyue Sun, Guangwei Wei, Siying Li, Zhongxi Zhao.  (2019)  A Nucleoside/Nucleobase-Rich Extract from Cordyceps Sinensis Inhibits the Epithelial–Mesenchymal Transition and Protects against Renal Fibrosis in Diabetic Nephropathy.  MOLECULES,  24  (22): (4119).  [PMID:31739543] [10.3390/molecules24224119]
11. Tianpei Cai, Haijuan Zhang, Jia Chen, Zhan Li, Hongdeng Qiu.  (2019)  Polyethyleneimine-functionalized carbon dots and their precursor co-immobilized on silica for hydrophilic interaction chromatography.  JOURNAL OF CHROMATOGRAPHY A,  1597  (142).  [PMID:30922720] [10.1016/j.chroma.2019.03.026]
12. Tengda Wang, Jianxing Shan, Li Wang, Xiangwen Zhang, Guozhu Li.  (2019)  On the kinetics of catalytic hydrogenation over Pd nanoparticles regulated by various nucleosides.  CHEMICAL ENGINEERING SCIENCE,  201  (15).  [PMID:] [10.1016/j.ces.2019.02.014]
13. Cai Tianpei, Zhang Haijuan, Rahman A. F. M. Mustafizur, Shi Yan-Ping, Qiu Hongdeng.  (2017)  Silica grafted with silanized carbon dots as a nano-on-micro packing material with enhanced hydrophilic selectivity.  MICROCHIMICA ACTA,  184  (8): (2629-2636).  [PMID:] [10.1007/s00604-017-2277-1]
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15. Qing Wang, Yao Long, Lin Yao, Li Xu, Zhi-Guo Shi, Lanying Xu.  (2016)  Preparation, characterization and application of a reversed phase liquid chromatography/hydrophilic interaction chromatography mixed-mode C18-DTT stationary phase.  TALANTA,  146  (442).  [PMID:26695288] [10.1016/j.talanta.2015.09.009]
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