mAb (C3); Mouse anti Human TBP Antibody; WB; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab130268-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $56.90 | |
| Ab130268-20μl | 20μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $754.90 | |
| Ab130268-50μl | 50μl | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $159.90 | |
| Ab130268-100μl | 100μl | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $239.90 | |
| Ab130268-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $1,799.90 |
mAb (C3); Mouse anti Human TBP Antibody; WB; Unconjugated
| Product Name | TBP Mouse mAb - Primary antibody, specific to TBP, Mouse IgG2a |
|---|---|
| Synonyms | GTF2D antibody; GTF2D1 antibody; HDL4 antibody; MGC117320 antibody; MGC126054 antibody; MGC126055 antibody; SCA17 antibody; TATA binding factor antibody; TATA box factor antibody; TATA sequence binding protein antibody; TATA sequence-binding protein antib |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, Azide Free, Lot by Lot |
| Host species | Mouse |
| Specificity | TBP |
| Immunogen | Recombinant TBP expressed in E.coli (AA 25-339). |
| Positive Control | WB: Jurkat, HEK293, HeLa, K562, C6, NIH/3T3 cell lysates. |
| Conjugation | Unconjugated |
| Grade | Azide Free, Carrier Free, ExactAb™, Validated |
| Product Description | Mouse anti Human TBP Antibody, Monoclonal (C3), could be used for WB and so on. |
| Isotype | Mouse IgG2a |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A+G Affinity Chromatography |
| Form | Liquid |
| Concentration | Lot by Lot |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (12 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
TBP Mouse mAb (Ab130268) - Western Blot
All lanes: TBP Mouse mAb (Ab130268) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution
Predicted band size: 38 kDa
Observed band size: 43&36 kDa
Exposure time: 5.1 s
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB | 0.5-2 µg/mL |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F0800899 | Certificate of Analysis | Aug 25, 2023 | Ab130268 |
| ZJ23F0800900 | Certificate of Analysis | Aug 25, 2023 | Ab130268 |
| 1. Lee SK, Anzick SL, Choi JE, Bubendorf L, Guan XY, Jung YK, Kallioniemi OP, Kononen J, Trent JM, Azorsa D, Jhun BH, Cheong JH, Lee YC, Meltzer PS, Lee JW. (1999) A nuclear factor, ASC-2, as a cancer-amplified transcriptional coactivator essential for ligand-dependent transactivation by nuclear receptors in vivo.. J Biol Chem, 274 (48): (34283-93). [PMID:10567404] |
| 2. Kao, C C CC and 5 more authors.. (1990) Cloning of a transcriptionally active human TATA binding factor.. Science (New York, N.Y.), (29): [PMID:2194289] |
| 3. Peterson, M G MG, Tanese, N N, Pugh, B F BF and Tjian, R R.. (1990) Functional domains and upstream activation properties of cloned human TATA binding protein.. Science (New York, N.Y.), (29): [PMID:2363050] |
| 4. Hoffman, A A and 6 more authors.. (1990) Highly conserved core domain and unique N terminus with presumptive regulatory motifs in a human TATA factor (TFIID).. Nature, (26): [PMID:2374612] |
| 5. Veschambre, P P, Simard, P P and Jalinot, P P.. (1995) Evidence for functional interaction between the HIV-1 Tat transactivator and the TATA box binding protein in vivo.. Journal of molecular biology, (7): [PMID:7608968] |
| 6. Comai, L L and 5 more authors.. (1994) Reconstitution of transcription factor SL1: exclusive binding of TBP by SL1 or TFIID subunits.. Science (New York, N.Y.), (23): [PMID:7801123] |
| 7. Kashanchi, F F and 7 more authors.. (1994) Direct interaction of human TFIID with the HIV-1 transactivator tat.. Nature, (20): [PMID:8121496] |
| 8. Ha, I I and 6 more authors.. (1993) Multiple functional domains of human transcription factor IIB: distinct interactions with two general transcription factors and RNA polymerase II.. Genes & development, [PMID:8504927] |
| 9. Nikolov, D B DB and 5 more authors.. (1996) Crystal structure of a human TATA box-binding protein/TATA element complex.. Proceedings of the National Academy of Sciences of the United States of America, (14): [PMID:8643494] |
| 10. Juo, Z S ZS and 5 more authors.. (1996) How proteins recognize the TATA box.. Journal of molecular biology, (16): [PMID:8757291] |
| 11. Fukushima, A A and 5 more authors.. (1998) Characterization of functional domains of an embryonic stem cell coactivator UTF1 which are conserved and essential for potentiation of ATF-2 activity.. The Journal of biological chemistry, (2): [PMID:9748258] |
| 12. LeRoy, G G, Orphanides, G G, Lane, W S WS and Reinberg, D D.. (1998) Requirement of RSF and FACT for transcription of chromatin templates in vitro.. Science (New York, N.Y.), (4): [PMID:9836642] |
| 13. Rao, S S, Matsumura, A A, Yoon, J J and Simon, M C MC.. (1999) SPI-B activates transcription via a unique proline, serine, and threonine domain and exhibits DNA binding affinity differences from PU.1.. The Journal of biological chemistry, (16): [PMID:10196196] |
| 14. Eberhard, D D and Busslinger, M M.. (1999) The partial homeodomain of the transcription factor Pax-5 (BSAP) is an interaction motif for the retinoblastoma and TATA-binding proteins.. Cancer research, (1): [PMID:10197586] |
| 15. Chang, C H CH, Scott, G K GK, Baldwin, M A MA and Benz, C C CC.. (1999) Exon 4-encoded acidic domain in the epithelium-restricted Ets factor, ESX, confers potent transactivating capacity and binds to TATA-binding protein (TBP).. Oncogene, (24): [PMID:10391676] |
| 16. Koide, R R and 8 more authors.. (1999) A neurological disease caused by an expanded CAG trinucleotide repeat in the TATA-binding protein gene: a new polyglutamine disease?. Human molecular genetics, [PMID:10484774] |
| 17. Tsai, F T FT and Sigler, P B PB.. (2000) Structural basis of preinitiation complex assembly on human pol II promoters.. The EMBO journal, (4): [PMID:10619841] |
| 18. Yuan, C X CX and Gurley, W B WB.. (2000) Potential targets for HSF1 within the preinitiation complex.. Cell stress & chaperones, [PMID:11005381] |
| 19. Zühlke, C C and 8 more authors.. (2001) Different types of repeat expansion in the TATA-binding protein gene are associated with a new form of inherited ataxia.. European journal of human genetics : EJHG, [PMID:11313753] |
| 20. Gangloff, Y G YG and 9 more authors.. (2001) The TFIID components human TAF(II)140 and Drosophila BIP2 (TAF(II)155) are novel metazoan homologues of yeast TAF(II)47 containing a histone fold and a PHD finger.. Molecular and cellular biology, [PMID:11438666] |
| 21. Nakamura, K K and 8 more authors.. (2001) SCA17, a novel autosomal dominant cerebellar ataxia caused by an expanded polyglutamine in TATA-binding protein.. Human molecular genetics, (1): [PMID:11448935] |
| 22. Kamada, K K and 7 more authors.. (2001) Crystal structure of negative cofactor 2 recognizing the TBP-DNA transcription complex.. Cell, (13): [PMID:11461703] |
| 23. Cabart, P P and Murphy, S S.. (2001) BRFU, a TFIIB-like factor, is directly recruited to the TATA-box of polymerase III small nuclear RNA gene promoters through its interaction with TATA-binding protein.. The Journal of biological chemistry, (16): [PMID:11564744] |
| 24. Silveira, I I and 24 more authors.. (2002) Trinucleotide repeats in 202 families with ataxia: a small expanded (CAG)n allele at the SCA17 locus.. Archives of neurology, [PMID:11939898] |
| 25. Wang, P Jeremy PJ and Page, David C DC.. (2002) Functional substitution for TAF(II)250 by a retroposed homolog that is expressed in human spermatogenesis.. Human molecular genetics, (15): [PMID:12217962] |
| 26. Hinkley, Craig S CS, Hirsch, Heather A HA, Gu, Liping L, LaMere, Brandon B and Henry, R William RW.. (2003) The small nuclear RNA-activating protein 190 Myb DNA binding domain stimulates TATA box-binding protein-TATA box recognition.. The Journal of biological chemistry, (16): [PMID:12621023] |
| 27. Raha, Tamal T, Cheng, S W Grace SW and Green, Michael R MR.. (2005) HIV-1 Tat stimulates transcription complex assembly through recruitment of TBP in the absence of TAFs.. PLoS biology, [PMID:15719058] |
| 28. Friedrich, J Karsten JK, Panov, Kostya I KI, Cabart, Pavel P, Russell, Jackie J and Zomerdijk, Joost C B M JC.. (2005) TBP-TAF complex SL1 directs RNA polymerase I pre-initiation complex formation and stabilizes upstream binding factor at the rDNA promoter.. The Journal of biological chemistry, (19): [PMID:15970593] |
| 29. Di Pietro, Cinzia C and 22 more authors.. (2007) Genomics, evolution, and expression of TBPL2, a member of the TBP family.. DNA and cell biology, [PMID:17570761] |
| 30. Liu, Lifeng L and 9 more authors.. (2009) MCAF1/AM is involved in Sp1-mediated maintenance of cancer-associated telomerase activity.. The Journal of biological chemistry, (20): [PMID:19106100] |
| 31. Gouge, Jerome J and 8 more authors.. (2015) Redox Signaling by the RNA Polymerase III TFIIB-Related Factor Brf2.. Cell, (3): [PMID:26638071] |
| 32. Louder, Robert K RK and 5 more authors.. (2016) Structure of promoter-bound TFIID and model of human pre-initiation complex assembly.. Nature, (31): [PMID:27007846] |
| 33. He, Yuan Y and 6 more authors.. (2016) Near-atomic resolution visualization of human transcription promoter opening.. Nature, (19): [PMID:27193682] |
| 34. Zhang, Mudan and 13 more authors.. (2018) Herpes Simplex Virus Type 2 Infection-Induced Expression of CXCR3 Ligands Promotes CD4+ T Cell Migration and Is Regulated by the Viral Immediate-Early Protein ICP4.. Frontiers in immunology, [PMID:30619292] |
