| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab131263-10μg | 10μg | In stock | $59.90 | |
| Ab131263-50μg | 50μg | In stock | $169.90 | |
| Ab131263-100μg | 100μg | In stock | $219.90 | |
| Ab131263-1mg | 1mg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $1,999.90 |
mAb (F38-2E2); Mouse anti Human TIM 3/CD366 Antibody; Flow; Unconjugated
| Product Name | TIM 3/CD366 Mouse mAb - Primary antibody, specific to TIM 3/CD366, Mouse IgG1 | |
|---|---|---|
| Synonyms | CD366 | FLJ14428 | HAVCR2 | HAVcr-2 | hepatitis A virus cellular receptor 2 | kidney injury molecule-3 | KIM-3 | SPTCL | T cell immunoglobulin mucin 3 | T cell immunoglobulin mucin-3 | T-cell immunoglobulin and mucin domain-containing protein 3 | TIM 3 | | |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, Azide Free, Lot by Lot | |
| Host species | Mouse | |
| Specificity | TIM 3/CD366 | |
| Immunogen | Human Tim-3 fusion protein | |
| Positive Control | Flow: Human peripheral blood lymphocytes. | |
| Conjugation | Unconjugated | |
| Grade | Azide Free, Carrier Free, ExactAb™, Validated | |
| Product Description | Mouse anti Human TIM 3/CD366 Antibody, Monoclonal (F38-2E2), could be used for Flow and so on. Application:
Protein Function Cell surface receptor implicated in modulating innate and adaptive immune responses. Generally accepted to have an inhibiting function. Reports on stimulating functions suggest that the activity may be influenced by the cellular context and/or the respective ligand (PubMed:24825777). Regulates macrophage activation (PubMed:11823861). Inhibits T-helper type 1 lymphocyte (Th1)-mediated auto- and alloimmune responses and promotes immunological tolerance (PubMed:14556005). In CD8+ cells attenuates TCR-induced signaling, specifically by blocking NF-kappaB and NFAT promoter activities resulting in the loss of IL-2 secretion. The function may implicate its association with LCK proposed to impair phosphorylation of TCR subunits, and/or LGALS9-dependent recruitment of PTPRC to the immunological synapse (PubMed:24337741, PubMed:26492563). In contrast, shown to activate TCR-induced signaling in T-cells probably implicating ZAP70, LCP2, LCK and FYN (By similarity). Expressed on Treg cells can inhibit Th17 cell responses (PubMed:24838857). Receptor for LGALS9 (PubMed:16286920, PubMed:24337741). Binding to LGALS9 is believed to result in suppression of T-cell responses; the resulting apoptosis of antigen-specific cells may implicate HAVCR2 phosphorylation and disruption of its association with BAG6. Binding to LGALS9 is proposed to be involved in innate immune response to intracellular pathogens. Expressed on Th1 cells interacts with LGALS9 expressed on Mycobacterium tuberculosis-infected macrophages to stimulate antibactericidal activity including IL-1 beta secretion and to restrict intracellular bacterial growth (By similarity). However, the function as receptor for LGALS9 has been challenged (PubMed:23555261). Also reported to enhance CD8+ T-cell responses to an acute infection such as by Listeria monocytogenes (By similarity). Receptor for phosphatidylserine (PtSer); PtSer-binding is calcium-dependent. May recognize PtSer on apoptotic cells leading to their phagocytosis. Mediates the engulfment of apoptotic cells by dendritic cells. Expressed on T-cells, promotes conjugation but not engulfment of apoptotic cells. Expressed on dendritic cells (DCs) positively regulates innate immune response and in synergy with Toll-like receptors promotes secretion of TNF-alpha. In tumor-imfiltrating DCs suppresses nucleic acid-mediated innate immune repsonse by interaction with HMGB1 and interfering with nucleic acid-sensing and trafficking of nucleid acids to endosomes (By similarity). Expressed on natural killer (NK) cells acts as a coreceptor to enhance IFN-gamma production in response to LGALS9 (PubMed:22323453). In contrast, shown to suppress NK cell-mediated cytotoxicity (PubMed:22383801). Negatively regulates NK cell function in LPS-induced endotoxic shock. |
| Isotype | Mouse IgG1 |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein G purified |
| Form | Liquid |
| Concentration | Lot by Lot |
| Storage Temp | Store at 2-8°C |
| Shipped In | Wet ice |
| Stability And Storage | Shipped at 4°C. Store at 4°C long term (12 months). Upon delivery aliquot. |
TIM 3/CD366 Mouse mAb (Ab131263) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood monocytes labelling TIM 3/CD366 with TIM 3/CD366 Mouse mAb (Ab131263) conjugated with biotin (right panel) at 0.1 μg/mL for 1 hour at 4°C, compared with mouse IgG (Ab170221) conjugated with biotin - Isotype Control (left panel) and detected with SA-PE (rp156250).
TIM 3/CD366 Mouse mAb (Ab131263) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood monocytes labelling TIM 3/CD366 (red) with TIM 3/CD366 Mouse mAb (Ab131263) conjugated with biotin at 0.1 μg/mL for 1 hour at 4°C, and detected with SA-PE (rp156250). Blue - Isotype control, mouse IgG (Ab170221) conjugated with biotin. Black - Unlabelled control, cells without incubation with primary antibody.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
| Application | Dilution info |
|---|---|
| Flow Cytometry | 1μg/100w cells "> 1μg/100w cells |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0404401 | Certificate of Analysis | Apr 10, 2024 | Ab131263 |
| ZJ24F0404400 | Certificate of Analysis | Apr 10, 2024 | Ab131263 |
| ZJ24F0404399 | Certificate of Analysis | Apr 10, 2024 | Ab131263 |
