| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab131327-10μl | 10μl | In stock | $66.90 | |
| Ab131327-50μl | 50μl | In stock | $189.90 | |
| Ab131327-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $299.90 | |
| Ab131327-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,809.90 |
pAb; Rabbit anti Human TIMP1 Antibody; WB; Unconjugated
| Product Name | TIMP1 Antibody - Primary antibody, specific to TIMP1, Rabbit IgG |
|---|---|
| Synonyms | Clgi antibody | Collagenase inhibitor antibody | Collagenase inhibitor, Human antibody | EPA antibody | EPO antibody | Erythroid Potentiating Activity antibody | Erythroid-potentiating activity antibody | Fibroblast collagenase inhibitor antibody | FLJ903 |
| Specifications & Purity | ExactAb™, Validated, 2.6 mg/mL |
| Host species | Rabbit |
| Specificity | TIMP1 |
| Immunogen | A synthetic peptide derived from human TIMP1 (AA 1-207). |
| Positive Control | WB: HT-29, SK-OV-3 and HeLa cell lysates. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Validated |
| Product Description | Rabbit anti Human TIMP1 Antibody, Polyclonal antibody, could be used for WB and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Form | Liquid |
| Concentration | 2.6 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
TIMP1 Antibody (Ab131327) - Western Blot
All lanes: TIMP1 Antibody (Ab131327) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 23 kDa
Observed band size: 28 kDa
Exposure time: 1.0 s
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000"> 1/500-1/1000 |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Chart | Certificate Type | Date | Item |
|---|---|---|---|---|
 ZJ24F0403964 | Certificate of Analysis | Apr 01, 2024 | Ab131327 | |
| ZJ24F0403965 | Certificate of Analysis | Apr 01, 2024 | Ab131327 |
| 1. O'Shea, M M and 7 more authors.. (1992) Site-directed mutations that alter the inhibitory activity of the tissue inhibitor of metalloproteinases-1: importance of the N-terminal region between cysteine 3 and cysteine 13.. Biochemistry, (27): [PMID:1420137] |
| 2. Van Ranst, M M and 7 more authors.. (1991) The cytokine-protease connection: identification of a 96-kD THP-1 gelatinase and regulation by interleukin-1 and cytokine inducers.. Cytokine, [PMID:1653055] |
| 3. Osthues, A A, Knäuper, V V, Oberhoff, R R, Reinke, H H and Tschesche, H H.. (1992) Isolation and characterization of tissue inhibitors of metalloproteinases (TIMP-1 and TIMP-2) from human rheumatoid synovial fluid.. FEBS letters, (13): [PMID:1730286] |
| 4. Williamson, R A RA and 9 more authors.. (1990) Disulphide bond assignment in human tissue inhibitor of metalloproteinases (TIMP).. The Biochemical journal, (1): [PMID:2163605] |
| 5. Rapp, G G and 6 more authors.. (1990) Characterization of three abundant mRNAs from human ovarian granulosa cells.. DNA and cell biology, [PMID:2171551] |
| 6. Carmichael, D F DF and 6 more authors.. (1986) Primary structure and cDNA cloning of human fibroblast collagenase inhibitor.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:3010309] |
| 7. Docherty, A J AJ and 7 more authors.. (1985) Sequence of human tissue inhibitor of metalloproteinases and its identity to erythroid-potentiating activity.. Nature, [PMID:3903517] |
| 8. Opbroek, A A, Kenney, M C MC and Brown, D D.. (1993) Characterization of a human corneal metalloproteinase inhibitor (TIMP-1).. Current eye research, [PMID:7507419] |
| 9. Knäuper, V V, López-Otin, C C, Smith, B B, Knight, G G and Murphy, G G.. (1996) Biochemical characterization of human collagenase-3.. The Journal of biological chemistry, (19): [PMID:8576151] |
| 10. Knäuper, V V and 7 more authors.. (1997) The role of the C-terminal domain of human collagenase-3 (MMP-13) in the activation of procollagenase-3, substrate specificity, and tissue inhibitor of metalloproteinase interaction.. The Journal of biological chemistry, (21): [PMID:9065415] |
| 11. Hardcastle, A J AJ, Thiselton, D L DL, Nayudu, M M, Hampson, R M RM and Bhattacharya, S S SS.. (1997) Genomic organization of the human TIMP-1 gene. Investigation of a causative role in the pathogenesis of X-linked retinitis pigmentosa 2.. Investigative ophthalmology & visual science, [PMID:9286280] |
| 12. Gomis-Rüth, F X FX and 11 more authors.. (1997) Mechanism of inhibition of the human matrix metalloproteinase stromelysin-1 by TIMP-1.. Nature, (4): [PMID:9288970] |
| 13. Wu, B B and 7 more authors.. (2000) NMR structure of tissue inhibitor of metalloproteinases-1 implicates localized induced fit in recognition of matrix metalloproteinases.. Journal of molecular biology, (14): [PMID:10623524] |
| 14. Zhang, Zemin Z and Henzel, William J WJ.. (2004) Signal peptide prediction based on analysis of experimentally verified cleavage sites.. Protein science : a publication of the Protein Society, [PMID:15340161] |
| 15. Lewandrowski, Urs U, Moebius, Jan J, Walter, Ulrich U and Sickmann, Albert A.. (2006) Elucidation of N-glycosylation sites on human platelet proteins: a glycoproteomic approach.. Molecular & cellular proteomics : MCP, [PMID:16263699] |
| 16. Liu, Tao T and 6 more authors.. () Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry.. Journal of proteome research, [PMID:16335952] |
| 17. Ramachandran, Prasanna P and 5 more authors.. (2006) Identification of N-linked glycoproteins in human saliva by glycoprotein capture and mass spectrometry.. Journal of proteome research, [PMID:16740002] |
| 18. Jung, Ki-Kyung KK, Liu, Xu-Wen XW, Chirco, Rosemarie R, Fridman, Rafael R and Kim, Hyeong-Reh Choi HR.. (2006) Identification of CD63 as a tissue inhibitor of metalloproteinase-1 interacting cell surface protein.. The EMBO journal, (6): [PMID:16917503] |
| 19. Iyer, Shalini S, Wei, Shuo S, Brew, Keith K and Acharya, K Ravi KR.. (2007) Crystal structure of the catalytic domain of matrix metalloproteinase-1 in complex with the inhibitory domain of tissue inhibitor of metalloproteinase-1.. The Journal of biological chemistry, (5): [PMID:17050530] |
| 20. Grossman, Moran M and 6 more authors.. (2010) The intrinsic protein flexibility of endogenous protease inhibitor TIMP-1 controls its binding interface and affects its function.. Biochemistry, (27): [PMID:20545310] |
| 21. Batra, Jyotica J and 5 more authors.. (2012) Matrix metalloproteinase-10 (MMP-10) interaction with tissue inhibitors of metalloproteinases TIMP-1 and TIMP-2: binding studies and crystal structure.. The Journal of biological chemistry, (4): [PMID:22427646] |
| 22. Rosenow, Anja A and 6 more authors.. (2012) Resveratrol-induced changes of the human adipocyte secretion profile.. Journal of proteome research, (7): [PMID:22905912] |
| 23. Toricelli, Mariana M, Melo, Fabiana H M FH, Peres, Giovani B GB, Silva, Débora C P DC and Jasiulionis, Miriam G MG.. (2013) Timp1 interacts with beta-1 integrin and CD63 along melanoma genesis and confers anoikis resistance by activating PI3-K signaling pathway independently of Akt phosphorylation.. Molecular cancer, (25): [PMID:23522389] |
| 24. Lee, Soo Youn SY and 9 more authors.. (2014) TIMP-1 modulates chemotaxis of human neural stem cells through CD63 and integrin signalling.. The Biochemical journal, (1): [PMID:24635319] |
| 25. Tagliabracci, Vincent S VS and 14 more authors.. (2015) A Single Kinase Generates the Majority of the Secreted Phosphoproteome.. Cell, (18): [PMID:26091039] |
| 26. Gasson, J C JC and 9 more authors.. (1985) Molecular characterization and expression of the gene encoding human erythroid-potentiating activity.. Nature, [PMID:3839290] |
