| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab131343-10μl | 10μl | In stock | $66.90 | |
| Ab131343-50μl | 50μl | In stock | $199.90 | |
| Ab131343-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $299.90 | |
| Ab131343-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,499.90 |
mAb (1554CT494.262.47); Mouse anti Human TIMP2 Antibody; WB, IHC, ICC, IF, Flow; Unconjugated
| Product Name | TIMP2 Mouse mAb - Primary antibody, specific to TIMP2, Mouse IgG1 |
|---|---|
| Synonyms | CSC 21K antibody | CSC-21K antibody | CSC21K antibody | Metalloproteinase inhibitor 2 antibody | Metalloproteinase inhibitor 2 precursor antibody | TIMP 2 antibody | TIMP metallopeptidase inhibitor 2 antibody | TIMP-2 antibody | TIMP2 antibody | TIMP2_HUM |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, 0.5 mg/mL |
| Host species | Mouse |
| Specificity | TIMP2 |
| Immunogen | Recombinant protein of human TIMP2 expressed in E.coli (AA 1-220). |
| Positive Control | WB: HeLa, A431, HUVEC and A549 cell lysates. IHC: Human kidney tissue. ICC/IF: A549 cells. Flow: K562 cells. |
| Conjugation | Unconjugated |
| Grade | Carrier Free, ExactAb™, Validated |
| Product Description |
Mouse anti Human TIMP2 Antibody, Monoclonal (1554CT494.262.47), could be used for WB, IHC, ICC, IF, Flow and so on. |
| Isotype | Mouse IgG1 |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein G purified |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
TIMP2 Mouse mAb (Ab131343) - Western Blot
All lanes: TIMP2 Mouse mAb (Ab131343) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution
Predicted band size: 24 kDa
Observed band size: 21 kDa
Exposure time: 120.0 s
TIMP2 Mouse mAb (Ab131343) - IF/ICC
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A549 (human lung adenocarcinoma epithelial cell line) cells labeling TIMP2 with TIMP2 Mouse mAb (Ab131343) at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-mouse IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on A549 cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1/100 dilution (red). The nuclear counter stain is DAPI (blue).
TIMP2 Mouse mAb (Ab131343) - IHC
Immunohistochemical analysis of paraffin-embedded Human kidney section using TIMP2 Mouse mAb (Ab131343). TIMP2 Mouse mAb (Ab131343) was diluted at 1/200 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
TIMP2 Mouse mAb (Ab131343) - IHC
TIMP2 Mouse mAb (Ab131343) staining TIMP2 in human kidney sections by Immunohistochemistry. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH 6.0). Samples were incubated with TIMP2 Mouse mAb (Ab131343) (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
TIMP2 Mouse mAb (Ab131343) - Flow Cytometry
Overlay histogram showing K562 cells stained with TIMP2 Mouse mAb (Ab131343) (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the TIMP2 Mouse mAb (Ab131343) (1/25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was mouse IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10000 events was performed.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| Flow Cytometry | 1/25 |
| WB | 1/2000 "> 1/2000 |
| IHC | 1/200
|
| IF/ICC |
1/25
|
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Chart | Certificate Type | Date | Item |
|---|---|---|---|---|
 ZJ24F0102591 | Certificate of Analysis | Jan 25, 2024 | Ab131343 | |
| ZJ24F0102590 | Certificate of Analysis | Jan 25, 2024 | Ab131343 |
| 1. Stetler-Stevenson, W G WG, Krutzsch, H C HC and Liotta, L A LA.. (1992) TIMP-2: identification and characterization of a new member of the metalloproteinase inhibitor family.. Matrix (Stuttgart, Germany). Supplement, [PMID:1480041] |
| 2. Osthues, A A, Knäuper, V V, Oberhoff, R R, Reinke, H H and Tschesche, H H.. (1992) Isolation and characterization of tissue inhibitors of metalloproteinases (TIMP-1 and TIMP-2) from human rheumatoid synovial fluid.. FEBS letters, (13): [PMID:1730286] |
| 3. Boone, T C TC, Johnson, M J MJ, De Clerck, Y A YA and Langley, K E KE.. (1990) cDNA cloning and expression of a metalloproteinase inhibitor related to tissue inhibitor of metalloproteinases.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:2157214] |
| 4. Stetler-Stevenson, W G WG, Brown, P D PD, Onisto, M M, Levy, A T AT and Liotta, L A LA.. (1990) Tissue inhibitor of metalloproteinases-2 (TIMP-2) mRNA expression in tumor cell lines and human tumor tissues.. The Journal of biological chemistry, (15): [PMID:2380196] |
| 5. Goldberg, G I GI and 5 more authors.. (1989) Human 72-kilodalton type IV collagenase forms a complex with a tissue inhibitor of metalloproteases designated TIMP-2.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:2554304] |
| 6. Stetler-Stevenson, W G WG, Krutzsch, H C HC and Liotta, L A LA.. (1989) Tissue inhibitor of metalloproteinase (TIMP-2). A new member of the metalloproteinase inhibitor family.. The Journal of biological chemistry, (15): [PMID:2793861] |
| 7. Williamson, R A RA and 6 more authors.. (1994) Solution structure of the active domain of tissue inhibitor of metalloproteinases-2. A new member of the OB fold protein family.. Biochemistry, (4): [PMID:7918391] |
| 8. De Clerck, Y A YA, Darville, M I MI, Eeckhout, Y Y and Rousseau, G G GG.. (1994) Characterization of the promoter of the gene encoding human tissue inhibitor of metalloproteinases-2 (TIMP-2).. Gene, (25): [PMID:8112602] |
| 9. Hammani, K K and 6 more authors.. (1996) Structure and characterization of the human tissue inhibitor of metalloproteinases-2 gene.. The Journal of biological chemistry, (11): [PMID:8810321] |
| 10. Muskett, F W FW and 5 more authors.. (1998) High resolution structure of the N-terminal domain of tissue inhibitor of metalloproteinases-2 and characterization of its interaction site with matrix metalloproteinase-3.. The Journal of biological chemistry, (21): [PMID:9705310] |
| 11. Tuuttila, A A and 8 more authors.. (1998) Three-dimensional structure of human tissue inhibitor of metalloproteinases-2 at 2.1 A resolution.. Journal of molecular biology, (11): [PMID:9837731] |
| 12. Morgunova, Ekaterina E, Tuuttila, Ari A, Bergmann, Ulrich U and Tryggvason, Karl K.. (2002) Structural insight into the complex formation of latent matrix metalloproteinase 2 with tissue inhibitor of metalloproteinase 2.. Proceedings of the National Academy of Sciences of the United States of America, (28): [PMID:12032297] |
| 13. Batra, Jyotica J, Soares, Alexei S AS, Mehner, Christine C and Radisky, Evette S ES.. (2013) Matrix metalloproteinase-10/TIMP-2 structure and analyses define conserved core interactions and diverse exosite interactions in MMP/TIMP complexes.. PloS one, [PMID:24073280] |
