| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab132305-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $69.90 | |
| Ab132305-50μl | 50μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $199.90 | |
| Ab132305-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $319.90 | |
| Ab132305-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,199.90 |
mAb (OTI3E5); Mouse anti Human TRAP alpha/TRAPA Antibody; WB, IHC, IF/ICC, Flow Cytometry; Unconjugated
| Product Name | TRAP alpha/TRAPA Mouse mAb |
|---|---|
| Synonyms | DKFZp781N23103 antibody | FLJ14232 antibody | FLJ22100 antibody | FLJ23034 antibody | FLJ78242 antibody | FLJ93042 antibody | PGP35 antibody | Signal sequence receptor alpha antibody | Signal sequence receptor alpha subunit antibody | Signal sequence rece |
| Specifications & Purity | KO Validation, ExactAb™, Validated, See COA |
| Biochemical and Physiological Mechanisms | TRAP proteins are part of a complex whose function is to bind calcium to the ER membrane and thereby regulate the retention of ER resident proteins. May be involved in the recycling of the translocation apparatus after completion of the translocation pro |
| Host species | Mouse |
| Specificity | SSR1 |
| Immunogen | Recombinant full length protein of human TRAP alpha/TRAPA. |
| Positive Control | WB: Hep G2, HeLa, A549, Jurkat and A431 cell lysates. IHC: Human ovary adenocarcinoma, pancreas, endometrium, endometrium adenocarcinoma, prostate tissues. IF/ICC: COS7 cells transiently transfected with pCMV6-ENTRY SSR1. Flow Cytometry: HEK293T cells tra |
| Conjugation | Unconjugated |
| Grade | ExactAb™, KO Validation, Validated |
| Product Description | Mouse anti Human TRAP alpha/TRAPA Antibody, Monoclonal (OTI3E5), could be used for WB, IHC, IF/ICC, Flow Cytometry and so on. |
| SDS-PAGE | 150 kDa |
|---|---|
| Purification Method | Protein G purified |
| Form | Liquid |
| Concentration | See COA |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (12 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
TRAP alpha/TRAPA Mouse mAb (Ab132305) - Western Blot
All lanes: TRAP alpha/TRAPA Mouse mAb (Ab132305) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 32 kDa
Observed band size: 34 kDa
Exposure time: 120.0 s
TRAP alpha/TRAPA Mouse mAb (Ab132305) - Western Blot
Equivalent amounts of cell lysates (10 ug perlane) of wild-type HeLa cells and SSR1-Knockout HeLa cells were separated by SDS-PAGE and immunoblotted with TRAP alpha/TRAPA Mouse mAb (Ab132305) (1/2000). Then the blotted membrane was stripped and reprobed with anti- HSP90 antibody as a loading control.
TRAP alpha/TRAPA Mouse mAb (Ab132305) - IHC
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using TRAP alpha/TRAPA Mouse mAb (Ab132305). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min, Ab132305)
TRAP alpha/TRAPA Mouse mAb (Ab132305) - IHC
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using TRAP alpha/TRAPA Mouse mAb (Ab132305). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min, Ab132305)
TRAP alpha/TRAPA Mouse mAb (Ab132305) - IHC
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using TRAP alpha/TRAPA Mouse mAb (Ab132305). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min, Ab132305)
TRAP alpha/TRAPA Mouse mAb (Ab132305) - IHC
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using TRAP alpha/TRAPA Mouse mAb (Ab132305). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min, Ab132305)
TRAP alpha/TRAPA Mouse mAb (Ab132305) - IHC
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using TRAP alpha/TRAPA Mouse mAb (Ab132305). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min, Ab132305)
TRAP alpha/TRAPA Mouse mAb (Ab132305) - IF/ICC
TRAP alpha/TRAPA Mouse mAb (Ab132305) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SSR1.
TRAP alpha/TRAPA Mouse mAb (Ab132305) - Flow Cytometry
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by TRAP alpha/TRAPA Mouse mAb (Ab132305) and then analyzed by flow cytometry.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/2000 "> 1/500-1/2000 |
| Flow Cytometry | 1/100 "> 1/100 |
| IF/ICC | 1/100 "> 1/100 |
| IHC | 1/150"> 1/150 |
Enter Lot Number to search for COA:
