Inactive tyrphostin. Negative control for tyrphostins such as AG 490 . Inhibits CD40 signaling pathway. Reduces IL-12 production in macrophages. Blocks CD40L-induced translocation of NFκB to the nucleus. Shows antiallergic effects in vivo.
Storage Temp
Store at 2-8°C
Shipped In
Wet ice
Grade
Moligand™
Action Type
INHIBITOR
Mechanism of action
Inhibitor of epidermal growth factor receptor
Note
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour. Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
Product Description
Product description：
Tyrphostin A1(AG9) inhibits CD40L-stimulated IL-12 production in macrophage cultures and antigen-induced generation of Th1 cells. IC50 value: Target: IL-12 production inhibitor Addition of increasing concentration of A1 resulted in a dose dependent decrease of IL-12 p40, with maximal inhibition (62.5%) occurring at a dose of 10 μM. tyrphostin A1 blocks CD40L-induced translocation of NF-κB to the nucleus, and reduces the activation of IL-12 p40 gene. In vivo therapy with A1 leads to decrease in generation of myelin basic protein (MBP) specific encephalitogenic T cells. In addition, treatment of SJL/J mice with A1 results in attenuation of experimental allergic encephalomyelitis (EAE) . Tyrphostin A1 is a much weaker inhibitor of TK than other tyrphostins (IC50>1250 μM for epidermal growth factor receptor (EGFR) kinase), and therefore often used to differentiate TK-mediated effects of tyrphostins from other non-specific effects.
Application：
Tyrphostin 1 has been used to study its effect on the increase of transgene expression in HeLa and NIH3T3 cells.
1.Gazit A, Yaish P, Gilon C, Levitzki A. (1989) Tyrphostins I: synthesis and biological activity of protein tyrosine kinase inhibitors.. J Med Chem, 32 (10):(2344-52). [PMID:2552117]
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G2308354