Standard phospholipase C inhibitor (PLC) (IC 50 = ~6 µM at PLC-β3). Shows a variety of biological activities including antiplatelet activation, Ca 2+ signaling modulation and inhibition of 5-lipoxygenase activity. Active in vivo .
Storage Temp
Store at -20°C
Shipped In
Ice chest + Ice pads
Grade
Moligand™
Note
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour. Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
Product Description
Application：
It is used as a phospholipase C, phospholipase A2, and 5-LO inhibitor. It is also determined that in SK-N-SH neuroblastoma cells, U-73122 inhibits agonist-induced down-regulation of muscarinic receptors. In addition, it is a useful tool to investigate receptor-mediated PI turnover in signal transduction. U-73122 is a potent inhibitor of human neutrophil adhesion to biological surfaces (IC50 = 50 nM) as well as adhesion-dependent granule exocytosis and oxidative burst. U-73343 (sc-201422) is useful as a negative control for investigations of U-73122 phospholipase C antagonism and its cellular consequences.
1.Luo M et al.. (2020) Sanguinarine Rapidly Relaxes Rat Airway Smooth Muscle Cells Dependent on TAS2R Signaling.. Biol Pharm Bull, 43 (7):(1027-1034). [PMID:32404582]
References
1.Kulebyakin K et al.. (2022) Dynamic Balance between PTH1R-Dependent Signal Cascades Determines Its Pro- or Anti-Osteogenic Effects on MSC.. Cells, 11 (21):[PMID:36359914]
2.Kárpáti A et al.. (2018) Histamine elicits glutamate release from cultured astrocytes.. J Pharmacol Sci, [PMID:29858014]
3.Li H et al.. (2020) Internalization of trophoblastic small extracellular vesicles and detection of their miRNA cargo in P-bodies.. J Extracell Vesicles, 9 (1812261). [PMID:32944196]
4.Luo R et al.. (2019) Impaired calcium homeostasis via advanced glycation end products promotes apoptosis through endoplasmic reticulum stress in human nucleus pulposus cells and exacerbates intervertebral disc degeneration in rats.. FEBS J, 286 (21):(4356-4373). [PMID:31230413]
5.Abdoul-Azize S et al.. (2017) Improvement of dexamethasone sensitivity by chelation of intracellular Ca2+ in pediatric acute lymphoblastic leukemia cells through the prosurvival kinase ERK1/2 deactivation.. Oncotarget, 8 (16):(27339-27352). [PMID:28423696]
6.Mizuno K et al.. (2020) Role of Ca2+ transients at the node of the mouse embryo in breaking of left-right symmetry.. Sci Adv, 6 (30):(eaba1195). [PMID:32743070]
7.Tsai CN et al.. (2019) Serine 229 Balances the Hepatitis C Virus Nonstructural Protein NS5A between Hypo- and Hyperphosphorylated States.. J Virol, 93 (23):[PMID:31511391]
8.Luo M et al.. (2020) Sanguinarine Rapidly Relaxes Rat Airway Smooth Muscle Cells Dependent on TAS2R Signaling.. Biol Pharm Bull, 43 (7):(1027-1034). [PMID:32404582]
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B2419130