| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab100767-10μl | 10μl | In stock | $109.90 | |
| Ab100767-50μl | 50μl | In stock | $319.90 | |
| Ab100767-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $489.90 | |
| Ab100767-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,599.90 |
pAb; Rabbit anti Human UBA1 Antibody; WB, IHC; Unconjugated
| Product Name | UBA1 Antibody - Primary antibody, specific to UBA1, Rabbit IgG |
|---|---|
| Synonyms | EC 6.2.1.45 | Protein A1S9 | Ubiquitin-activating enzyme E1 | A1S9 antibody | A1S9 protein antibody | A1S9T and BN75 temperature sensitivity complementing antibody | A1S9T antibody | A1ST antibody | AMCX1 antibody | CFAP124 antibody | CTD-2522E6.1 antibod |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, 0.5 mg/mL |
| Host species | Rabbit |
| Specificity | UBA1 |
| Immunogen | A KLH conjugated synthetic peptide from the N-terminal region of human UBA1 (AA 1-30) |
| Positive Control | WB: K562, HeLa, Jurkat, HEK293, NIH/3T3 and C6 cell lysates. IHC: Human testis tissue. |
| Conjugation | Unconjugated |
| Grade | Carrier Free, ExactAb™, Validated |
| Product Description | Rabbit anti Human UBA1 Antibody, Polyclonal, could be used for WB, IHC and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified + Peptide affinity purified |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
UBA1 Antibody (Ab100767) Western Blot
All lanes: UBA1 Antibody (Ab100767) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab176443) at 1/20000 dilution
Predicted band size: 118 kDa
Observed band size: 116 kDa
Exposure time: 6.5 s
UBA1 Antibody (Ab100767) - IHC
UBA1 Antibody (Ab100767) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of UBA1 Antibody (Ab100767) for immunohistochemistry. Clinical relevance has not been evaluated.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
| Application | Dilution info |
|---|---|
| IHC | 1/100-1/500 |
| WB |
1/1000
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1/1000
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Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0303276 | Certificate of Analysis | Mar 14, 2024 | Ab100767 |
| ZJ24F0303275 | Certificate of Analysis | Mar 14, 2024 | Ab100767 |
| 1. Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M. (2009) Lysine acetylation targets protein complexes and co-regulates major cellular functions.. Science, 325 (5942): (834-40). [PMID:19608861] [10.1021/op500134e] |
| 2. Tan L, Gurbani D, Weisberg EL, Jones DS, Rao S, Singer WD, Bernard FM, Mowafy S, Jenney A, Du G et al.. (2017) Studies of TAK1-centered polypharmacology with novel covalent TAK1 inhibitors.. Bioorg Med Chem, 25 (4): (1320-1328). [PMID:28038940] [10.1021/op500134e] |
| 3. Sharon Gauci,Andreas O Helbig,Monique Slijper,Jeroen Krijgsveld,Albert J R Heck,Shabaz Mohammed. (2009-05-06) Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.. Analytical chemistry, 81 ((11)): (4493-4501). [PMID:19413330] |
| 4. Wang RE, Hunt CR, Chen J, Taylor JS.. (2011) Biotinylated quercetin as an intrinsic photoaffinity proteomics probe for the identification of quercetin target proteins.. Bioorg Med Chem, 19 (16): (4710-4720). [PMID:21798748] [10.1016/j.bmc.2011.07.005] |
| 5. Wu KJ, Zhong HJ, Li G, Liu C, Wang HD, Ma DL, Leung CH.. (2018) Structure-based identification of a NEDD8-activating enzyme inhibitor via drug repurposing.. Eur J Med Chem, 143 (1021-1027). [PMID:29232579] [10.1016/j.ejmech.2017.11.101] |
| 6. Itoh Y, Suzuki M.. (2018) Design, synthesis, and biological evaluation of novel ubiquitin-activating enzyme inhibitors.. Bioorg Med Chem Lett, 28 (16): (2723-2727). [PMID:29548576] [10.1016/j.bmcl.2018.03.004] |
| 7. Schwartz, A L AL, Trausch, J S JS, Ciechanover, A A, Slot, J W JW and Geuze, H H.. (1992) Immunoelectron microscopic localization of the ubiquitin-activating enzyme E1 in HepG2 cells.. Proceedings of the National Academy of Sciences of the United States of America, (15): [PMID:1376922] |
| 8. Ayusawa, D D and 7 more authors.. (1992) Complementation by a cloned human ubiquitin-activating enzyme E1 of the S-phase-arrested mouse FM3A cell mutant with thermolabile E1.. Cell structure and function, [PMID:1606621] |
| 9. . (1991) Molecular cloning, sequence, and tissue distribution of the human ubiquitin-activating enzyme E1.. Proceedings of the National Academy of Sciences of the United States of America, (15): [PMID:1871145] |
| 10. Handley, P M PM, Mueckler, M M, Siegel, N R NR, Ciechanover, A A and Schwartz, A L AL.. (1991) Molecular cloning, sequence, and tissue distribution of the human ubiquitin-activating enzyme E1.. Proceedings of the National Academy of Sciences of the United States of America, (1): [PMID:1986373] |
| 11. Zacksenhaus, E E and Sheinin, R R.. (1990) Molecular cloning, primary structure and expression of the human X linked A1S9 gene cDNA which complements the ts A1S9 mouse L cell defect in DNA replication.. The EMBO journal, [PMID:2390975] |
| 12. Handley-Gearhart, P M PM, Stephen, A G AG, Trausch-Azar, J S JS, Ciechanover, A A and Schwartz, A L AL.. (1994) Human ubiquitin-activating enzyme, E1. Indication of potential nuclear and cytoplasmic subpopulations using epitope-tagged cDNA constructs.. The Journal of biological chemistry, (30): [PMID:7528747] |
| 13. Rush, John J and 9 more authors.. (2005) Immunoaffinity profiling of tyrosine phosphorylation in cancer cells.. Nature biotechnology, [PMID:15592455] |
| 14. Ross, Mark T MT and 281 more authors.. (2005) The DNA sequence of the human X chromosome.. Nature, (17): [PMID:15772651] |
| 15. Giannakopoulos, Nadia V NV and 9 more authors.. (2005) Proteomic identification of proteins conjugated to ISG15 in mouse and human cells.. Biochemical and biophysical research communications, (21): [PMID:16139798] |
| 16. Allen, Elizabeth E and 6 more authors.. (2005) Gigaxonin-controlled degradation of MAP1B light chain is critical to neuronal survival.. Nature, (10): [PMID:16227972] |
| 17. Ramser, Juliane J and 11 more authors.. (2008) Rare missense and synonymous variants in UBE1 are associated with X-linked infantile spinal muscular atrophy.. American journal of human genetics, [PMID:18179898] |
| 18. Dephoure, Noah N and 6 more authors.. (2008) A quantitative atlas of mitotic phosphorylation.. Proceedings of the National Academy of Sciences of the United States of America, (5): [PMID:18669648] |
| 19. Mayya, Viveka V and 7 more authors.. (2009) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.. Science signaling, (18): [PMID:19690332] |
| 20. Rigbolt, Kristoffer T G KT and 9 more authors.. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.. Science signaling, (15): [PMID:21406692] |
| 21. Moudry, Pavel P and 6 more authors.. (2012) Ubiquitin-activating enzyme UBA1 is required for cellular response to DNA damage.. Cell cycle (Georgetown, Tex.), (15): [PMID:22456334] |
| 22. Van Damme, Petra P and 15 more authors.. (2012) N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB.. Proceedings of the National Academy of Sciences of the United States of America, (31): [PMID:22814378] |
| 23. Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4): [PMID:23186163] |
| 24. Dlamini, Nomazulu N and 12 more authors.. (2013) Clinical and neuropathological features of X-linked spinal muscular atrophy (SMAX2) associated with a novel mutation in the UBA1 gene.. Neuromuscular disorders : NMD, [PMID:23518311] |
| 25. and Xie, Shu-Tao ST.. (2014) Expression, purification, and crystal structure of N-terminal domains of human ubiquitin-activating enzyme (E1).. Bioscience, biotechnology, and biochemistry, [PMID:25209502] |
| 26. Beck, David B and 61 more authors.. (2020) Somatic Mutations in UBA1 and Severe Adult-Onset Autoinflammatory Disease.. The New England journal of medicine, (31): [PMID:33108101] |
| 27. Thomas R Burkard,Melanie Planyavsky,Ines Kaupe,Florian P Breitwieser,Tilmann Bürckstümmer,Keiryn L Bennett,Giulio Superti-Furga,Jacques Colinge. (2011-01-29) Initial characterization of the human central proteome.. BMC systems biology, 5 (17-17). [PMID:21269460] |
