Basic Description

Product Name	UBE2C Antibody - Primary antibody, specific to UBE2C, Rabbit IgG
Synonyms	Cyclin selective ubiquitin carrier protein antibody \| dJ447F3.2 antibody \| Mitotic specific ubiquitin conjugating enzyme antibody \| UB E2C antibody \| UBCH 10 antibody \| UbcH10 antibody \| UBE 2C antibody \| Ube2c antibody \| UBE2C_HUMAN antibody \| Ubiquitin
Specifications & Purity	ExactAb™, Validated, Carrier Free, High performance, Lot by Lot
Host species	Rabbit
Specificity	UBE2C
Immunogen	Recombinant UBE2C expressed in E.coli (AA 2-179).
Positive Control	WB: HeLa, A431, MCF7 and RAW 264.7 cell lysates. ICC/IF: HeLa cells.
Conjugation	Unconjugated
Grade	Carrier Free, ExactAb™, High Performance, Validated
Note	20μl卖完停产，不再备货
Product Description	Rabbit anti Human UBE2C Antibody, Polyclonal, could be used for WB, ICC, IF and so on. Application · WB: 0.01-2μg/mL · ICC/IF: 5-20μg/mL Protein Function Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'- and 'Lys-48'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by initiating 'Lys-11'-linked polyubiquitin chains on APC/C substrates, leading to the degradation of APC/C substrates by the proteasome and promoting mitotic exit.

Product Properties

Isotype	Rabbit IgG
SDS-PAGE	150 kDa
Purification Method	Antigen affinity purified + Protein A purified
Form	Liquid
Concentration	Lot by Lot
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at 4°C short term (1-2 weeks). Store at -20°C long term (24months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

UBE2C Antibody (Ab133227) - Western Blot
All lanes: UBE2C Antibody (Ab133227) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution

Predicted band size: 20 kDa
Observed band size: 17, 19 kDa
Exposure time: 5.0 s

UBE2C Antibody (Ab133227) - IF/ICC
FITC staining on IF
Samples: Human HeLa Cells
Primary Ab: 20 µg/mL UBE2C Antibody (Ab133227).

Associated Targets(Human)

UBE2C Tbio Ubiquitin-conjugating enzyme E2 C (0 Activities)

Discover Target: UBE2C

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mechanisms of Action

Mechanism of Action	Action Type	target ID	Target Name	Target Type	Target Organism	Binding Site Name	References

Application

Application	Dilution info
WB	0.01-2 µg/mL
IF/ICC	5-20 µg/mL

Certificates

Certificate of Analysis(COA)

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3 results found

Lot Number	Certificate Type	Date	Item
ZJ24F0404094	Certificate of Analysis	Apr 02, 2024	Ab133227
ZJ24F0404093	Certificate of Analysis	Apr 02, 2024	Ab133227
ZJ24F0404092	Certificate of Analysis	Apr 02, 2024	Ab133227

FAQs and Articles

FAQ

Immunopeptide blocking assay

Categories: Protocols

Tags:

Immunopeptideblocking PolyclonalAntibody SpecificBinding

Immunofluorescence Staining Protocol (Methanol permeabilization)

Categories: Protocols

Tags:

Frozen section Immunofluorescence staining Tissue section Methanol permeability IF

References

1. Deloukas P, Matthews LH, Ashurst J, Burton J, Gilbert JG, Jones M, Stavrides G, Almeida JP, Babbage AK, Bagguley CL, Bailey J, Barlow KF, Bates KN, Beard LM, Beare DM, Beasley OP, Bird CP, Blakey SE, Bridgeman AM, Brown AJ, Buck D, Burrill W, Butler AP, Carder C, Carter NP, Chapman JC, Clamp M, Clark G, Clark LN, Clark SY, Clee CM, Clegg S, Cobley VE, Collier RE, Connor R, Corby NR, Coulson A, Coville GJ, Deadman R, Dhami P, Dunn M, Ellington AG, Frankland JA, Fraser A, French L, Garner P, Grafham DV, Griffiths C, Griffiths MN, Gwilliam R, Hall RE, Hammond S, Harley JL, Heath PD, Ho S, Holden JL, Howden PJ, Huckle E, Hunt AR, Hunt SE, Jekosch K, Johnson CM, Johnson D, Kay MP, Kimberley AM, King A, Knights A, Laird GK, Lawlor S, Lehvaslaiho MH, Leversha M, Lloyd C, Lloyd DM, Lovell JD, Marsh VL, Martin SL, McConnachie LJ, McLay K, McMurray AA, Milne S, Mistry D, Moore MJ, Mullikin JC, Nickerson T, Oliver K, Parker A, Patel R, Pearce TA, Peck AI, Phillimore BJ, Prathalingam SR, Plumb RW, Ramsay H, Rice CM, Ross MT, Scott CE, Sehra HK, Shownkeen R, Sims S, Skuce CD, Smith ML, Soderlund C, Steward CA, Sulston JE, Swann M, Sycamore N, Taylor R, Tee L, Thomas DW, Thorpe A, Tracey A, Tromans AC, Vaudin M, Wall M, Wallis JM, Whitehead SL, Whittaker P, Willey DL, Williams L, Williams SA, Wilming L, Wray PW, Hubbard T, Durbin RM, Bentley DR, Beck S, Rogers J. (2001) The DNA sequence and comparative analysis of human chromosome 20.. Nature, 414 (6866): (865-71). [PMID:11780052] [10.1021/op500134e]
2. Sharon Gauci,Andreas O Helbig,Monique Slijper,Jeroen Krijgsveld,Albert J R Heck,Shabaz Mohammed. (2009-05-06) Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.. Analytical chemistry, 81 ((11)): (4493-4501). [PMID:19413330]
3. Chen H, Wu G, Gao S, Guo R, Zhao Z, Yuan H, Liu S, Wu J, Lu X, Yuan X, Yu Z, Zu X, Xie N, Yang N, Hu Z, Sun Q, Zhang W.. (2017) Discovery of Potent Small-Molecule Inhibitors of Ubiquitin-Conjugating Enzyme UbcH5c from α-Santonin Derivatives.. J Med Chem, 60 (16): (6828-6852). [PMID:28696694] [10.1021/acs.jmedchem.6b01829]
4. Ni S, Chen X, Yu Q, Xu Y, Hu Z, Zhang J, Zhang W, Li B, Yang X, Mao F, Huang J, Sun Y, Li J, Jia L.. (2020) Discovery of candesartan cilexetic as a novel neddylation inhibitor for suppressing tumor growth.. Eur J Med Chem, 185 (111848-111848). [PMID:31732254] [10.1016/j.ejmech.2019.111848]
5. Townsley, F M FM, Aristarkhov, A A, Beck, S S, Hershko, A A and Ruderman, J V JV.. (1997) Dominant-negative cyclin-selective ubiquitin carrier protein E2-C/UbcH10 blocks cells in metaphase.. Proceedings of the National Academy of Sciences of the United States of America, (18): [PMID:9122200]
6. Jin, Lingyan L, Williamson, Adam A, Banerjee, Sudeep S, Philipp, Isabelle I and Rape, Michael M.. (2008) Mechanism of ubiquitin-chain formation by the human anaphase-promoting complex.. Cell, (16): [PMID:18485873]
7. Garnett, Mathew J MJ and 7 more authors.. (2009) UBE2S elongates ubiquitin chains on APC/C substrates to promote mitotic exit.. Nature cell biology, [PMID:19820702]
8. Williamson, Adam A and 5 more authors.. (2009) Identification of a physiological E2 module for the human anaphase-promoting complex.. Proceedings of the National Academy of Sciences of the United States of America, (27): [PMID:19822757]
9. David, Yael Y, Ziv, Tamar T, Admon, Arie A and Navon, Ami A.. (2010) The E2 ubiquitin-conjugating enzymes direct polyubiquitination to preferred lysines.. The Journal of biological chemistry, (19): [PMID:20061386]
10. Rigbolt, Kristoffer T G KT and 9 more authors.. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.. Science signaling, (15): [PMID:21406692]

Solution Calculators

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SKU	Size	Availability	Price
Ab133227-10μl	10μl	In stock	$59.90
Ab133227-50μl	50μl	In stock	$179.90
Ab133227-100μl	100μl	In stock	$279.90
Ab133227-1ml	1ml	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$1,999.90

UBE2C Antibody - Primary antibody, specific to UBE2C, Rabbit IgG