mAb (K06328_5A8); Mouse anti Human USH1C/Harmonin Antibody; WB, IHC; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab133668-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $69.90 | |
| Ab133668-50μl | 50μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $199.90 | |
| Ab133668-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $319.90 | |
| Ab133668-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,599.90 |
mAb (K06328_5A8); Mouse anti Human USH1C/Harmonin Antibody; WB, IHC; Unconjugated
| Product Name | USH1C/Harmonin Mouse mAb |
|---|---|
| Synonyms | AIE 75antibody | AIE75 antibody | Antigen NY CO 38/NY CO 37 antibody | Antigen NY-CO-38/NY-CO-37 antibody | Autoimmune enteropathy related antigen AIE 75 antibody | Autoimmune enteropathy related antigen AIE75 antibody | Autoimmune enteropathy-related ant |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, 1.0 mg/mL |
| Host species | Mouse |
| Specificity | USH1C/Harmonin |
| Immunogen | Recombinant protein of human USH1C/Harmonin (AA 301-550). |
| Positive Control | WB: Mouse kidney lysates. IHC: Human small intestine tissue. |
| Conjugation | Unconjugated |
| Grade | Carrier Free, ExactAb™, Validated |
| Product Description | Mouse anti Human USH1C/Harmonin Antibody, Monoclonal (K06328_5A8), could be used for WB, IHC and so on. |
| Isotype | Mouse IgG1 |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Form | Liquid |
| Concentration | 1.0 mg/mL |
| Storage Temp | Store at -80°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 2-8 ℃ (3 months). Store at -20 ℃ (12 months). Store at -80 ℃ (36 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
USH1C/Harmonin Mouse mAb (Ab133668) - Western Blot
All lanes: USH1C/Harmonin Mouse mAb (Ab133668) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 62 kDa
Observed band size: 67 kDa
Exposure time: 100.0 s
USH1C/Harmonin Mouse mAb (Ab133668) - IHC
IHC analysis of small intestine tissue by USH1C/Harmonin Mouse mAb (Ab133668).IHC was performed using sections of the formalin-fixed paraffin-embedded small intestine tissue. Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9.0 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H₂O₂ for 30 min at room temperature. The sections were then incubated with USH1C/Harmonin Mouse mAb (Ab133668) at 1 µg/mL at room temperature for 1 h. Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with the secondary antibody was used as the background control.Result: Glandular cells are positively stained at the cytoplasm and membrane.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB |
1/1000
|
| IHC | 1/1000 |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0708808 | Certificate of Analysis | Jul 25, 2024 | Ab133668 |
| ZJ24F0708809 | Certificate of Analysis | Jul 25, 2024 | Ab133668 |
