| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab133867-10μl | 10μl | In stock | $69.90 | |
| Ab133867-50μl | 50μl | In stock | $199.90 | |
| Ab133867-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $289.90 | |
| Ab133867-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,599.90 |
mAb (1414CT354.12.23.93); Mouse anti Human VAMP8/EDB Antibody; WB, IHC; Unconjugated
| Product Name | VAMP8/EDB Mouse mAb - Primary antibody, specific to VAMP8, Mouse IgG1 |
|---|---|
| Synonyms | EDB | Endobrevin | VAMP-8 | EDB antibody | Endobrevin antibody | VAMP 8 antibody | VAMP-8 antibody | VAMP8 antibody | VAMP8_HUMAN antibody | Vesicle associated membrane protein 8 antibody | Vesicle-associated membrane protein 8 antibody | EDB | VAMP-8 |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, High performance, 0.5 mg/mL |
| Host species | Mouse |
| Specificity | VAMP8 |
| Immunogen | A KLH conjugated synthetic peptide from the N-terminal region of human VAMP8 (AA 2-24). |
| Positive Control | WB: THP-1 and K562 cell lysates. IHC: Human brain tissue. |
| Conjugation | Unconjugated |
| Grade | Carrier Free, ExactAb™, High Performance, Validated |
| Product Description | Mouse anti Human VAMP8/EDB Antibody, Monoclonal (1414CT354.12.23.93), could be used for WB, IHC and so on. |
| Isotype | Mouse IgG1 |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein G purified |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
VAMP8/EDB Mouse mAb (Ab133867) - Western Blot
All lanes: VAMP8/EDB Mouse mAb (Ab133867) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 11 kDa
Observed band size: 15 kDa
Exposure time: 26.4 s
VAMP8/EDB Mouse mAb (Ab133867) - IHC
Immunohistochemical analysis of paraffin-embedded H. brain section using VAMP8/EDB Mouse mAb (Ab133867) (N-term). VAMP8/EDB Mouse mAb (Ab133867) was diluted at 1/25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| IHC | 1/100-1/500 |
| WB | 1/1000 - 1/2000"> 1/1000 - 1/2000 |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0403968 | Certificate of Analysis | Apr 01, 2024 | Ab133867 |
| ZJ24F0403969 | Certificate of Analysis | Apr 01, 2024 | Ab133867 |
| 1. Sharon Gauci,Andreas O Helbig,Monique Slijper,Jeroen Krijgsveld,Albert J R Heck,Shabaz Mohammed. (2009-05-06) Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.. Analytical chemistry, 81 ((11)): (4493-4501). [PMID:19413330] |
| 2. Wong, S H SH and 5 more authors.. (1998) Endobrevin, a novel synaptobrevin/VAMP-like protein preferentially associated with the early endosome.. Molecular biology of the cell, [PMID:9614193] |
| 3. Polgár, János J, Chung, Sul-Hee SH and Reed, Guy L GL.. (2002) Vesicle-associated membrane protein 3 (VAMP-3) and VAMP-8 are present in human platelets and are required for granule secretion.. Blood, (1): [PMID:12130530] |
| 4. Hillier, Ladeana W LW and 121 more authors.. (2005) Generation and annotation of the DNA sequences of human chromosomes 2 and 4.. Nature, (7): [PMID:15815621] |
| 5. Itakura, Eisuke E, Kishi-Itakura, Chieko C and Mizushima, Noboru N.. (2012) The hairpin-type tail-anchored SNARE syntaxin 17 targets to autophagosomes for fusion with endosomes/lysosomes.. Cell, (7): [PMID:23217709] |
| 6. Jean, Steve S, Cox, Sarah S, Nassari, Sonya S and Kiger, Amy A AA.. (2015) Starvation-induced MTMR13 and RAB21 activity regulates VAMP8 to promote autophagosome-lysosome fusion.. EMBO reports, [PMID:25648148] |
| 7. Diao, Jiajie J and 12 more authors.. (2015) ATG14 promotes membrane tethering and fusion of autophagosomes to endolysosomes.. Nature, (23): [PMID:25686604] |
| 8. Liu, Wang W and 10 more authors.. (2018) Nε-fatty acylation of multiple membrane-associated proteins by Shigella IcsB effector to modulate host function.. Nature microbiology, [PMID:30061757] |
| 9. Miao, Guangyan; Zhang, Yujie; Chen, Di and Zhang, Hong.. (2020) The ER-Localized Transmembrane Protein TMEM39A/SUSR2 Regulates Autophagy by Controlling the Trafficking of the PtdIns(4)P Phosphatase SAC1.. Molecular cell, (6): [PMID:31806350] |
| 10. Miao, Guangyan and 8 more authors.. (2021) ORF3a of the COVID-19 virus SARS-CoV-2 blocks HOPS complex-mediated assembly of the SNARE complex required for autolysosome formation.. Developmental cell, (22): [PMID:33422265] |
