| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab133998-10μl | 10μl | In stock | $66.90 | |
| Ab133998-50μl | 50μl | In stock | $189.90 | |
| Ab133998-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $289.90 | |
| Ab133998-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,809.90 |
pAb; Rabbit anti Human VDAC1/Porin Antibody; WB, IHC; Unconjugated
| Product Name | VDAC1/Porin Antibody - Primary antibody, specific to VDAC1/Porin, Rabbit IgG |
|---|---|
| Synonyms | N2441 antibody | OMP2 antibody | POR1 antibody | hVDAC1 antibody | MGC111064 antibody | Mitochondrial Porin antibody | Outer mitochondrial membrane protein porin 1 antibody | Plasmalemmal porin antibody | Porin 31HL antibody | Porin 31HM antibody | VDAC a |
| Specifications & Purity | ExactAb™, Validated, 0.1 mg/mL |
| Host species | Rabbit |
| Specificity | VDAC1/Porin |
| Immunogen | A synthetic peptide derived from human VDAC1 (AA 3-16). |
| Positive Control | WB: Raji, HEK293, A431, HeLa, THP-1, Jurkat, Hep G2, NIH/3T3 and C6 cell lysates. IHC: Human liver tissue. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Validated |
| Product Description |
Rabbit anti Human VDAC1/Porin Antibody, Polyclonal antibody, could be used for WB, IHC and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Form | Liquid |
| Concentration | 0.1 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
VDAC1/Porin Antibody (Ab133998) - Western Blot
All lanes: VDAC1/Porin Antibody (Ab133998) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 31 kDa
Observed band size: 31 kDa
Exposure time: 16.3 s
VDAC1/Porin Antibody (Ab133998) - IHC
Immunohistochemistry analysis of paraffin-embedded Human liver using VDAC1/Porin Antibody (Ab133998). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000"> 1/500-1/1000 |
| IHC | 1/50 - 1/100 "> 1/50 - 1/100 |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0202794 | Certificate of Analysis | Feb 23, 2024 | Ab133998 |
| ZJ24F0202793 | Certificate of Analysis | Feb 23, 2024 | Ab133998 |
| 1. Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M. (2009) Lysine acetylation targets protein complexes and co-regulates major cellular functions.. Science, 325 (5942): (834-40). [PMID:19608861] [10.1021/op500134e] |
| 2. Sebastian Hiller,Robert G Garces,Thomas J Malia,Vladislav Y Orekhov,Marco Colombini,Gerhard Wagner. (2008-08-30) Solution structure of the integral human membrane protein VDAC-1 in detergent micelles.. Science (New York, N.Y.), 321 ((5893)): (1206-1210). [PMID:18755977] |
| 3. Shashank Dadsena,Svenja Bockelmann,John G M Mina,Dina G Hassan,Sergei Korneev,Guilherme Razzera,Helene Jahn,Patrick Niekamp,Dagmar Müller,Markus Schneider,Fikadu G Tafesse,Siewert J Marrink,Manuel N Melo,Joost C M Holthuis. (2019-04-25) Ceramides bind VDAC2 to trigger mitochondrial apoptosis.. Nature communications, 10 ((1)): (1832-1832). [PMID:31015432] |
| 4. Sharon Gauci,Andreas O Helbig,Monique Slijper,Jeroen Krijgsveld,Albert J R Heck,Shabaz Mohammed. (2009-05-06) Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.. Analytical chemistry, 81 ((11)): (4493-4501). [PMID:19413330] |
| 5. Jürgens, L L and 6 more authors.. (1991) Studies on human porin. IV. The primary structures of "Porin 31HM" purified from human skeletal muscle membranes and of "Porin 31HL" derived from human B lymphocyte membranes are identical.. Biological chemistry Hoppe-Seyler, [PMID:1657034] |
| 6. Zhang, D W DW and Colombini, M M.. (1989) Inhibition by aluminum hydroxide of the voltage-dependent closure of the mitochondrial channel, VDAC.. Biochimica et biophysica acta, (25): [PMID:2469483] |
| 7. Kayser, H H and 6 more authors.. (1989) [Identification of human porins. II. Characterization and primary structure of a 31-lDa porin from human B lymphocytes (Porin 31HL)].. Biological chemistry Hoppe-Seyler, [PMID:2559745] |
| 8. Yu, W H WH, Wolfgang, W W and Forte, M M.. (1995) Subcellular localization of human voltage-dependent anion channel isoforms.. The Journal of biological chemistry, (9): [PMID:7539795] |
| 9. Thomas, L L, Blachly-Dyson, E E, Colombini, M M and Forte, M M.. (1993) Mapping of residues forming the voltage sensor of the voltage-dependent anion-selective channel.. Proceedings of the National Academy of Sciences of the United States of America, (15): [PMID:7685903] |
| 10. Blachly-Dyson, E E and 7 more authors.. (1993) Cloning and functional expression in yeast of two human isoforms of the outer mitochondrial membrane channel, the voltage-dependent anion channel.. The Journal of biological chemistry, (25): [PMID:8420959] |
| 11. Decker, W K WK, Bowles, K R KR, Schatte, E C EC, Towbin, J A JA and Craigen, W J WJ.. (1999) Revised fine mapping of the human voltage-dependent anion channel loci by radiation hybrid analysis.. Mammalian genome : official journal of the International Mammalian Genome Society, [PMID:10501981] |
| 12. Stadtmüller, U U and 12 more authors.. (1999) Mitochondria-derived and extra-mitochondrial human type-1 porin are identical as revealed by amino acid sequencing and electrophysiological characterisation.. Biological chemistry, [PMID:10661876] |
| 13. Messina, A A and 5 more authors.. (2000) Characterization of the human porin isoform 1 (HVDAC1) gene by amplification on the whole human genome: A tool for porin deficiency analysis.. Biochemical and biophysical research communications, (21): [PMID:10772903] |
| 14. Thinnes, F P FP and 9 more authors.. (2001) Gadolinium as an opener of the outwardly rectifying Cl(-) channel (ORCC). Is there relevance for cystic fibrosis therapy?. Pflugers Archiv : European journal of physiology, [PMID:11845315] |
| 15. Verrier, Florence F, Mignotte, Bernard B, Jan, Gwenaël G and Brenner, Catherine C.. (2003) Study of PTPC composition during apoptosis for identification of viral protein target.. Annals of the New York Academy of Sciences, [PMID:15033708] |
| 16. Schmutz, Jeremy J and 75 more authors.. (2004) The DNA sequence and comparative analysis of human chromosome 5.. Nature, (16): [PMID:15372022] |
| 17. Zamarin, Dmitriy D, García-Sastre, Adolfo A, Xiao, Xiaoyao X, Wang, Rong R and Palese, Peter P.. (2005) Influenza virus PB1-F2 protein induces cell death through mitochondrial ANT3 and VDAC1.. PLoS pathogens, [PMID:16201016] |
| 18. Zahedi, René P RP and 8 more authors.. (2008) Phosphoproteome of resting human platelets.. Journal of proteome research, [PMID:18088087] |
| 19. Daub, Henrik H and 9 more authors.. (2008) Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle.. Molecular cell, (8): [PMID:18691976] |
| 20. Bayrhuber, Monika M and 9 more authors.. (2008) Structure of the human voltage-dependent anion channel.. Proceedings of the National Academy of Sciences of the United States of America, (7): [PMID:18832158] |
| 21. Chen, Yumay Y, Gaczynska, Maria M, Osmulski, Pawel P, Polci, Rosaria R and Riley, Daniel J DJ.. (2010) Phosphorylation by Nek1 regulates opening and closing of voltage dependent anion channel 1.. Biochemical and biophysical research communications, (9): [PMID:20230784] |
| 22. Rigbolt, Kristoffer T G KT and 9 more authors.. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.. Science signaling, (15): [PMID:21406692] |
| 23. Bienvenut, Willy V WV and 6 more authors.. (2012) Comparative large scale characterization of plant versus mammal proteins reveals similar and idiosyncratic N-α-acetylation features.. Molecular & cellular proteomics : MCP, [PMID:22223895] |
| 24. Lau, Eric E and 7 more authors.. (2012) PKCε promotes oncogenic functions of ATF2 in the nucleus while blocking its apoptotic function at mitochondria.. Cell, (3): [PMID:22304920] |
| 25. Van Damme, Petra P and 15 more authors.. (2012) N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB.. Proceedings of the National Academy of Sciences of the United States of America, (31): [PMID:22814378] |
| 26. Zhang, Xiaoping X and 9 more authors.. (2012) Human Bop is a novel BH3-only member of the Bcl-2 protein family.. Protein & cell, [PMID:23055042] |
| 27. Fernandez-Echevarria, C C, Díaz, M M, Ferrer, I I, Canerina-Amaro, A A and Marin, R R.. (2014) Aβ promotes VDAC1 channel dephosphorylation in neuronal lipid rafts. Relevance to the mechanisms of neurotoxicity in Alzheimer's disease.. Neuroscience, (10): [PMID:25168729] |
| 28. Li, Lei L and 11 more authors.. (2014) Plasminogen kringle 5 induces endothelial cell apoptosis by triggering a voltage-dependent anion channel 1 (VDAC1) positive feedback loop.. The Journal of biological chemistry, (21): [PMID:25296756] |
| 29. Cunningham, Christian N CN and 8 more authors.. (2015) USP30 and parkin homeostatically regulate atypical ubiquitin chains on mitochondria.. Nature cell biology, [PMID:25621951] |
| 30. Ibeas Bih, Clementino C and 5 more authors.. (2015) Molecular Targets of Cannabidiol in Neurological Disorders.. Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics, [PMID:26264914] |
| 31. Shanmughapriya, Santhanam S and 21 more authors.. (2015) SPG7 Is an Essential and Conserved Component of the Mitochondrial Permeability Transition Pore.. Molecular cell, (1): [PMID:26387735] |
| 32. Marginedas-Freixa, I I and 14 more authors.. (2016) TSPO ligands stimulate ZnPPIX transport and ROS accumulation leading to the inhibition of P. falciparum growth in human blood.. Scientific reports, (19): [PMID:27641616] |
| 33. Marginedas-Freixa, Irene and 11 more authors.. (2018) Human erythrocytes release ATP by a novel pathway involving VDAC oligomerization independent of pannexin-1.. Scientific reports, (30): [PMID:30061676] |
| 34. Van Alstyne, Meaghan M, Lotti, Francesco F, Dal Mas, Andrea A, Area-Gomez, Estela E and Pellizzoni, Livio L.. (2018) Stasimon/Tmem41b localizes to mitochondria-associated ER membranes and is essential for mouse embryonic development.. Biochemical and biophysical research communications, (30): [PMID:30352685] |
| 35. Namba, Takushi.. (2019) BAP31 regulates mitochondrial function via interaction with Tom40 within ER-mitochondria contact sites.. Science advances, [PMID:31206022] |
