mAb (AB07/4H7); Mouse anti Vimentin Antibody; WB, Flow, ICC/IF, ELISA; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab179444-10μl | 10μl | In stock | $66.90 | |
| Ab179444-20μl | 20μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $99.90 | |
| Ab179444-50μl | 50μl | In stock | $199.90 | |
| Ab179444-100μl | 100μl | In stock | $299.90 | |
| Ab179444-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $1,499.90 |
mAb (AB07/4H7); Mouse anti Vimentin Antibody; WB, Flow, ICC/IF, ELISA; Unconjugated
| Product Name | Vimentin Mouse mAb - Primary antibody, specific to Vimentin, IgG2b |
|---|---|
| Synonyms | CTRCT30 antibody; Epididymis luminal protein 113 antibody; FLJ36605 antibody; HEL113 antibody; VIM antibody; VIME_HUMAN antibody; Vimentin antibody; Anti-Vimentin Antibody; Vimentin Monoclonal Antibody; Anti-Human VIM/Vimentin Antibody; Mouse anti-human V |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, Azide Free, High performance, 1.0 mg/mL |
| Host species | Mouse |
| Specificity | Vimentin |
| Immunogen | Recombinant full length protein corresponding to Human Vimentin |
| Positive Control | WB: HeLa, HEK293, K562, A549, Jurkat, SK-N-SH and HUVEC whole cell lysates Flow: HeLa cells ICC/IF: HeLa cells |
| Conjugation | Unconjugated |
| Grade | Azide Free, Carrier Free, ExactAb™, High Performance, Validated |
| Product Description | Application |
| Isotype | IgG2b |
|---|---|
| Light Chain Type | Kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Form | Liquid |
| Concentration | 1.0 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Upon delivery aliquot. Avoid freeze/thaw cycle. |
Vimentin Mouse mAb (Ab179444) - Western Blot
All lanes: Vimentin Antibody (Ab179444) at 1/10000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 53.7 kDa
Observed band size: 55.4 kDa
Exposure time: 25.0 s
Vimentin Mouse mAb (Ab179444) - Western Blot
All lanes: Vimentin Antibody (Ab179444) at 1/10000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 53.7 kDa
Observed band size: 55.5 kDa
Exposure time: 25.0 s
Vimentin Mouse mAb (Ab179444) - Western Blot
All lanes: Vimentin Antibody (Ab179444) at 1/10000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 53.7 kDa
Observed band size: 55.4 kDa
Exposure time: 10.1 s
Vimentin Mouse mAb (Ab179444) - Flow Cytometry
Overlay histogram showing HeLa cells stained with Vimentin Mouse mAb (Ab179444) (Red). Cells were fixed and permeabilized with 100% methanol. A Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. The mouse IgG (Ab170221) was used as an isotype control (Blue).
Vimentin Mouse mAb (Ab179444) - ICC/IF
IF analysis of Vimentin (Green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Vimentin Mouse mAb (Ab179444) at 2.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab156254) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| ICC/IF | 2 μg/mL |
| Flow |
0.008-1μg/mL
|
| WB | 1/2,000-1/10,000 ; Predicted molecular weight: 54 kDa |
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| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24R0400481 | Certificate of Analysis | Apr 26, 2024 | Ab179444 |
| ZJ24F0102679 | Certificate of Analysis | Feb 01, 2024 | Ab179444 |
| ZJ24F0102678 | Certificate of Analysis | Feb 01, 2024 | Ab179444 |
| ZJ24F0102677 | Certificate of Analysis | Feb 01, 2024 | Ab179444 |
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