| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab134698-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $69.90 | |
| Ab134698-50μl | 50μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $209.90 | |
| Ab134698-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $319.90 | |
| Ab134698-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,499.90 |
pAb; Rabbit anti Human WIPI2 Antibody; WB, IHC; Unconjugated
| Product Name | WIPI2 Antibody - Primary antibody, specific to WIPI2, Rabbit IgG |
|---|---|
| Synonyms | ATG18B antibody | Atg21 antibody | CGI 50 antibody | DKFZp434J154 antibody | DKFZp686P02188 antibody | FLJ12979 antibody | FLJ14217 antibody | FLJ42984 antibody | WD repeat domain phosphoinositide-interacting protein 2 antibody | WD repeat domain, phospho |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, 0.3 mg/mL |
| Host species | Rabbit |
| Specificity | WIPI2 |
| Immunogen | Fusion protein of human WIPI2 (AA 366-454) |
| Positive Control | WB: HeLa cell lysate. IHC: Human brain tissue. |
| Conjugation | Unconjugated |
| Grade | Carrier Free, ExactAb™, Validated |
| Product Description |
Rabbit anti Human WIPI2 Antibody, Polyclonal, could be used for WB, IHC and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Form | Liquid |
| Concentration | 0.3 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
WIPI2 Antibody (Ab134698) - Western Blot
All lanes: WIPI2 Antibody (Ab134698) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 49 kDa
Observed band size: 45 kDa
Exposure time: 200.0 s
WIPI2 Antibody (Ab134698) - IHC
Immunohistochemistry analysis of paraffin-embedded Human brain using WIPI2 Antibody (Ab134698). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000"> 1/500-1/1000 |
| IHC | 1/50 - 1/100 "> 1/50 - 1/100 |
Enter Lot Number to search for COA:
| 1. Zhao YG et al.. (2017) The ER-Localized Transmembrane Protein EPG-3/VMP1 Regulates SERCA Activity to Control ER-Isolation Membrane Contacts for Autophagosome Formation.. Mol Cell, 67 (6): (974-989.e6). [PMID:28890335] |
| 2. Lai, C H CH, Chou, C Y CY, Ch'ang, L Y LY, Liu, C S CS and Lin, W W.. (2000) Identification of novel human genes evolutionarily conserved in Caenorhabditis elegans by comparative proteomics.. Genome research, [PMID:10810093] |
| 3. Wiemann, S S and 23 more authors.. (2001) Toward a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs.. Genome research, [PMID:11230166] |
| 4. Hillier, Ladeana W LW and 106 more authors.. (2003) The DNA sequence of human chromosome 7.. Nature, (10): [PMID:12853948] |
| 5. Proikas-Cezanne, Tassula T and 5 more authors.. (2004) WIPI-1alpha (WIPI49), a member of the novel 7-bladed WIPI protein family, is aberrantly expressed in human cancer and is linked to starvation-induced autophagy.. Oncogene, (16): [PMID:15602573] |
| 6. Polson, Hannah E J HE and 6 more authors.. (2010) Mammalian Atg18 (WIPI2) localizes to omegasome-anchored phagophores and positively regulates LC3 lipidation.. Autophagy, [PMID:20505359] |
| 7. Proikas-Cezanne, Tassula T and Robenek, Horst H.. (2011) Freeze-fracture replica immunolabelling reveals human WIPI-1 and WIPI-2 as membrane proteins of autophagosomes.. Journal of cellular and molecular medicine, [PMID:21564513] |
| 8. Ogawa, Michinaga M and 17 more authors.. (2011) A Tecpr1-dependent selective autophagy pathway targets bacterial pathogens.. Cell host & microbe, (19): [PMID:21575909] |
| 9. Orsi, A A and 6 more authors.. (2012) Dynamic and transient interactions of Atg9 with autophagosomes, but not membrane integration, are required for autophagy.. Molecular biology of the cell, [PMID:22456507] |
| 10. Chauhan, Santosh S and 6 more authors.. (2013) ZKSCAN3 is a master transcriptional repressor of autophagy.. Molecular cell, (11): [PMID:23434374] |
| 11. Manzoni, Claudia C and 9 more authors.. (2013) Inhibition of LRRK2 kinase activity stimulates macroautophagy.. Biochimica et biophysica acta, [PMID:23916833] |
| 12. Dooley, Hannah C HC and 5 more authors.. (2014) WIPI2 links LC3 conjugation with PI3P, autophagosome formation, and pathogen clearance by recruiting Atg12-5-16L1.. Molecular cell, (17): [PMID:24954904] |
| 13. Bakula, Daniela D and 11 more authors.. (2017) WIPI3 and WIPI4 β-propellers are scaffolds for LKB1-AMPK-TSC signalling circuits in the control of autophagy.. Nature communications, (31): [PMID:28561066] |
| 14. Jelani, Musharraf M and 14 more authors.. (2019) A mutation in the major autophagy gene, WIPI2, associated with global developmental abnormalities.. Brain : a journal of neurology, (1): [PMID:30968111] |
