Skip to the beginning of the images gallery

Wnt-C59 (C59) - ≥98%, high purity , CAS No.1243243-89-1, Inhibitor of porcupine O-acyltransferase

Moligand™

≥98%

CAS#: 1243243-89-1
Formula: C₂₅H₂₁N₃O
Molecular Weight: 379.45
PubChem CID: 57519544

Item Number

W125232

Grouped product items
SKU	Size	Availability	Price
W125232-5mg	5mg	Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!	$59.90
W125232-25mg	25mg	Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!	$171.90
W125232-100mg	100mg	Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!	$477.90

Potent, cell-permeable Wnt signaling pathway inhibitor

View related series

porcupine O-acyltransferase Inhibitor

Basic Description

Synonyms	Wnt-C59 \| 1243243-89-1 \| 2-(4-(2-methylpyridin-4-yl)phenyl)-N-(4-(pyridin-3-yl)phenyl)acetamide \| C25H21N3O \| 2-[4-(2-Methyl-pyridin-4-yl)-phenyl]-N-(4-pyridin-3-yl-phenyl)-acetamide \| Wnt-C59 (C59) \| CHEMBL2139947 \| 2-[4-(2-methylpyridin-4-yl)phenyl]-N-(4-pyridin-3-yl
Specifications & Purity	Moligand™, ≥98%
Biochemical and Physiological Mechanisms	C59 inhibits PORCN activity in vitro at nanomolar concentrations, as assessed by inhibition of Wnt palmitoylation, Wnt interaction with the carrier protein Wntless/WLS, Wnt secretion, and Wnt activation of β-catenin reporter activity . Wnt-C59 was used
Storage Temp	Store at -20°C
Shipped In	Ice chest + Ice pads
Grade	Moligand™
Action Type	INHIBITOR
Mechanism of action	Inhibitor of porcupine O-acyltransferase
Note	Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour. Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
Product Description	Wnt-C59 (C59) is a PORCN inhibitor for Wnt3A-mediated activation of a multimerized TCF-binding site driving luciferase with IC50 of 74 pM in HEK293 cells. A porcupine homolog - Drosophila (PORCN) inhibitor for Wnt3A-mediated activation of a multimerized TCF-binding site driving luciferase

Associated Targets(Human)

PORCN Tchem Protein-serine O-palmitoleoyltransferase porcupine (1 Activities)

Discover Target: PORCN

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

YES1 Tclin Tyrosine-protein kinase YES (2781 Activities)

Discover Target: YES1

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

PORCN Tchem Probable protein-cysteine N-palmitoyltransferase porcupine (135 Activities)

Discover Target: PORCN

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

HEK-293T (167025 Activities)

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Associated Targets(non-human)

Hdac6 Histone deacetylase 6 (222 Activities)

Discover Target: Hdac6

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

rep Replicase polyprotein 1ab (378 Activities)

Discover Target: rep

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

SARS-CoV-2 (38078 Activities)

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Names and Identifiers

IUPAC Name	2-[4-(2-methylpyridin-4-yl)phenyl]-N-(4-pyridin-3-ylphenyl)acetamide
INCHI	InChI=1S/C25H21N3O/c1-18-15-22(12-14-27-18)20-6-4-19(5-7-20)16-25(29)28-24-10-8-21(9-11-24)23-3-2-13-26-17-23/h2-15,17H,16H2,1H3,(H,28,29)
InChi Key	KHZOJCQBHJUJFY-UHFFFAOYSA-N
Canonical SMILES	CC1=NC=CC(=C1)C2=CC=C(C=C2)CC(=O)NC3=CC=C(C=C3)C4=CN=CC=C4
Isomeric SMILES	CC1=NC=CC(=C1)C2=CC=C(C=C2)CC(=O)NC3=CC=C(C=C3)C4=CN=CC=C4
PubChem CID	57519544
Molecular Weight	379.45

PubChem CID

ChEMBL Ligand

BindingDB Ligand

CAS Registry No.

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section

1 results found

Lot Number	Certificate Type	Date	Item
E1712139	Certificate of Analysis	Jan 16, 2023	W125232

References

1. Ho SY, Keller TH. (2015) The use of porcupine inhibitors to target Wnt-driven cancers.. Bioorg Med Chem Lett, 25 (23): (5472-6). [PMID:26522946]
2. Kozielewicz P, Shekhani R, Moser S, Bowin CF, Wesslowski J, Davidson G, Schulte G. (2021) Quantitative Profiling of WNT-3A Binding to All Human Frizzled Paralogues in HEK293 Cells by NanoBiT/BRET Assessments.. ACS Pharmacol Transl Sci, 4 (3): (1235-1245). [PMID:34151213]
3. Arthofer E, Hot B, Petersen J, Strakova K, Jäger S, Grundmann M, Kostenis E, Gutkind JS, Schulte G. (2016) WNT Stimulation Dissociates a Frizzled 4 Inactive-State Complex with Gα12/13.. Mol Pharmacol, 90 (4): (447-59). [PMID:27458145]
4. Lin HK et al.. (2018) Caveolin-1 down-regulation is required for Wnt5a-Frizzled 2 signalling in Ha-RasV12 -induced cell transformation.. J Cell Mol Med, [PMID:29502342]
5. Willems L et al.. (2020) Differentiation of Human Cardiac Atrial Appendage Stem Cells into Adult Cardiomyocytes: A Role for the Wnt Pathway?. Int J Mol Sci, 21 (11): [PMID:32486259]
6. Lu W et al.. (2016) CXCL12/CXCR4 Axis Regulates Aggrecanase Activation and Cartilage Degradation in a Post-Traumatic Osteoarthritis Rat Model.. Int J Mol Sci, 17 (10): [PMID:27690009]
7. Valnohova J et al.. (2018) Functional dissection of the N-terminal extracellular domains of Frizzled 6 reveals their roles for receptor localization and Dishevelled recruitment.. J Biol Chem, 293 (46): (17875-17887). [PMID:30237173]
8. Takakura N et al.. (2019) Generation and characterization of Suncus murinus intestinal organoid: a useful tool for studying motilin secretion.. Cell Biol Int, [PMID:31293061]
9. Xian L et al.. (2017) HMGA1 amplifies Wnt signalling and expands the intestinal stem cell compartment and Paneth cell niche.. Nat Commun, 8 (15008). [PMID:28452345]
10. Marruecos L et al.. (2020) I?Ba deficiency imposes a fetal phenotype to intestinal stem cells.. EMBO Rep, 21 (6): (e49708). [PMID:32270911]
11. Zhou W et al.. (2020) miR-6838-5p Affects Cell Growth, Migration, and Invasion by Targeting GPRIN3 via the Wnt/ß-Catenin Signaling Pathway in Gastric Cancer.. Pathobiology, 87 (6): (327-337). [PMID:33254176]
12. Jobe NP et al.. (2021) Reduced WNT5A signaling in melanoma cells favors an amoeboid mode of invasion.. Mol Oncol, 15 (7): (1835-1848). [PMID:33969605]
13. Radaszkiewicz T et al.. (2021) RNF43 inhibits WNT5A-driven signaling and suppresses melanoma invasion and resistance to the targeted therapy.. Elife, 10 [PMID:34702444]
14. Shinkawa T et al.. (2022) Subtypes in pancreatic ductal adenocarcinoma based on niche factor dependency show distinct drug treatment responses.. J Exp Clin Cancer Res, 41 (89). [PMID:35272688]

Solution Calculators

Molarity Calculator

Determine the necessary mass, volume, or concentration for preparing a solution.

Dilution Calculator

Determine the dilution needed to prepare a stock solution.