pAb; Rabbit anti Human Wnt1 Antibody; WB, IHC, IF, ICC; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab134725-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $36.90 | |
| Ab134725-20μl | 20μl | In stock | $49.90 | |
| Ab134725-50μl | 50μl | In stock | $99.90 | |
| Ab134725-100μl | 100μl | In stock | $139.90 | |
| Ab134725-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $999.90 |
pAb; Rabbit anti Human Wnt1 Antibody; WB, IHC, IF, ICC; Unconjugated
| Product Name | Wnt1 Antibody - Primary antibody, specific to WNT1, Rabbit IgG |
|---|---|
| Synonyms | Int-1; INT1Proto-oncogene Int-1 homolog; proto-oncogene Wnt-1; wingless-type MMTV integration site family, member 1 (oncogene INT1); wingless-type MMTV integration site family, member 1; Wnt1; Wnt-1; BMND16 antibody; INT1 antibody; OI15 antibody; oncogene |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, High performance, Lot by Lot |
| Host species | Rabbit |
| Specificity | WNT1 |
| Immunogen | Recombinant WNT1 expressed in E.coli (AA 28-370) |
| Positive Control | WB: HeLa and Jurkat cell lysates. IHC: Human kidney tissue. IF/ICC: MCF7 cells. |
| Conjugation | Unconjugated |
| Grade | Carrier Free, ExactAb™, High performance, Validated |
| Product Description | Rabbit anti Human Wnt1 Antibody, Polyclonal, could be used for WB, IHC, IF, ICC and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Form | Liquid |
| Concentration | Lot by Lot |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Wnt1 Antibody (Ab134725) - Western Blot
All lanes: Wnt1 Antibody (Ab134725) at 1/5000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 40 kDa
Observed band size: 37 kDa
Exposure time: 42.0 s
Wnt1 Antibody (Ab134725) - IF/ICC
FITC staining on IF
Sample: Human MCF7 cells
Primary Ab: 20 μg/mL Wnt1 Antibody (Ab134725)
Second Ab: 1.5 μg/mL FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
Wnt1 Antibody (Ab134725) - IHC
DAB staining on IHC
Sample: Human Kidney Tissue
Primary Ab: 20 µg/mL Wnt1 Antibody (Ab134725)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| IHC | 5-20 µg/mL |
| WB |
0.01-2 µg/mL
|
| IF/ICC | 5-20µg/mL |
Enter Lot Number to search for COA:
To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F1202146 | Certificate of Analysis | Dec 20, 2023 | Ab134725 |
| ZJ23F1202147 | Certificate of Analysis | Dec 20, 2023 | Ab134725 |
| ZJ23F1202148 | Certificate of Analysis | Dec 20, 2023 | Ab134725 |
| 1. van Ooyen, A A, Kwee, V V and Nusse, R R.. (1985) The nucleotide sequence of the human int-1 mammary oncogene; evolutionary conservation of coding and non-coding sequences.. The EMBO journal, [PMID:2998762] |
| 2. Fahiminiya, Somayyeh S and 5 more authors.. (2013) Mutations in WNT1 are a cause of osteogenesis imperfecta.. Journal of medical genetics, [PMID:23434763] |
| 3. Keupp, Katharina K and 35 more authors.. (2013) Mutations in WNT1 cause different forms of bone fragility.. American journal of human genetics, (4): [PMID:23499309] |
| 4. Pyott, Shawna M SM and 14 more authors.. (2013) WNT1 mutations in families affected by moderately severe and progressive recessive osteogenesis imperfecta.. American journal of human genetics, (4): [PMID:23499310] |
| 5. Laine, Christine M CM and 22 more authors.. (2013) WNT1 mutations in early-onset osteoporosis and osteogenesis imperfecta.. The New England journal of medicine, (9): [PMID:23656646] |
| 6. Mihara, Emiko E and 7 more authors.. (2016) Active and water-soluble form of lipidated Wnt protein is maintained by a serum glycoprotein afamin/α-albumin.. eLife, (23): [PMID:26902720] |
| 7. Won, Joon Yeon JY and 7 more authors.. (2017) Novel missense loss-of-function mutations of WNT1 in an autosomal recessive Osteogenesis imperfecta patient.. European journal of medical genetics, [PMID:28528193] |
