A KLH conjugated synthetic peptide from the Central region of human WNT10B (AA 193-222).
Positive Control
WB: Mouse skeletal muscle, HeLa, Hep G2, MDA-MB-231 and HT-29 cell lysates.
Conjugation
Unconjugated
Grade
Azide Free, Carrier Free, ExactAb™, Validated
Product Description
Rabbit anti Human Wnt10b Antibody, Polyclonal, could be used for WB and so on. Application: WB: 1/2000 Protein Function Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters.
Product Properties
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Protein A purified + Peptide affinity purified
Form
Liquid
Concentration
0.5 mg/mL
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
Wnt10b Antibody (Ab134730) - Western Blot All lanes: Wnt10b Antibody (Ab134730) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 43 kDa Observed band size: 39, 46 kDa Exposure time: 73.7 s
1.Bui, T D TD and 7 more authors.. (1997) A novel human Wnt gene, WNT10B, maps to 12q13 and is expressed in human breast carcinomas.. Oncogene, (13):[PMID:9121776]
2.Hardiman, G G, Kastelein, R A RA and Bazan, J F JF.. (1997) Isolation, characterization and chromosomal localization of human WNT10B.. Cytogenetics and cell genetics, [PMID:9284937]
3.Bergstein, I I and 7 more authors.. (1997) Isolation of two novel WNT genes, WNT14 and WNT15, one of which (WNT15) is closely linked to WNT3 on human chromosome 17q21.. Genomics, (15):[PMID:9441749]
4.Saitoh, T T, Kirikoshi, H H, Mine, T T and Katoh, M M.. (2001) Proto-oncogene WNT10B is up-regulated by tumor necrosis factor alpha in human gastric cancer cell line MKN45.. International journal of oncology, [PMID:11713588]
5.Christodoulides, C C and 14 more authors.. (2006) WNT10B mutations in human obesity.. Diabetologia, [PMID:16477437]
6.Scherer, Steven E SE and 216 more authors.. (2006) The finished DNA sequence of human chromosome 12.. Nature, (16):[PMID:16541075]
7.Ugur, Sibel Aylin SA and Tolun, Aslihan A.. (2008) Homozygous WNT10b mutation and complex inheritance in Split-Hand/Foot Malformation.. Human molecular genetics, (1):[PMID:18515319]
8.Wend, P P, Wend, K K, Krum, S A SA and Miranda-Carboni, G A GA.. (2012) The role of WNT10B in physiology and disease.. Acta physiologica (Oxford, England), [PMID:21447090]
9.Mihara, Emiko E and 7 more authors.. (2016) Active and water-soluble form of lipidated Wnt protein is maintained by a serum glycoprotein afamin/α-albumin.. eLife, (23):[PMID:26902720]
10.Yu, Ping P and 15 more authors.. (2016) Mutations in WNT10B Are Identified in Individuals with Oligodontia.. American journal of human genetics, (7):[PMID:27321946]
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