pAb; Rabbit anti Human XIAP Antibody; WB, IHC, IF, ICC; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab134877-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $56.90 | |
| Ab134877-20μl | 20μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $89.90 | |
| Ab134877-50μl | 50μl | In stock | $169.90 | |
| Ab134877-100μl | 100μl | In stock | $279.90 | |
| Ab134877-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $1,669.90 |
pAb; Rabbit anti Human XIAP Antibody; WB, IHC, IF, ICC; Unconjugated
| Product Name | XIAP Antibody - Primary antibody, specific to XIAP |
|---|---|
| Synonyms | API3X-linked inhibitor of apoptosis protein | apoptosis inhibitor 3 | baculoviral IAP repeat-containing 4 | baculoviral IAP repeat-containing protein 4 | BIRC4 | BIRC4XLP2 | E3 ubiquitin-protein ligase XIAP | EC 6.3.2.- | hIAP3 | hIAP-3 | hILP | IAP3 | IA |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, Azide Free, High performance, Lot by Lot |
| Host species | Rabbit |
| Specificity | XIAP |
| Immunogen | Recombinant XIAP expressed in E.coli (AA 1-497) |
| Positive Control | WB: HeLa, Raji, MCF7, HT-29, A549, Jurkat, HepG2, MOLT-4, NIH/3T3 and C6 cell lysates. IHC: Human kidney, breast cancer tissues. IF/ICC: Hep G2, MCF7 cells. |
| Conjugation | Unconjugated |
| Grade | Azide Free, Carrier Free, ExactAb™, High Performance, Validated |
| Product Description | Rabbit anti Human XIAP Antibody, Polyclonal, could be used for WB, IHC, IF/ICCand so on. Application: WB: 0.01-3µg/mL IHC: 2-30µg/mL IF/ICC: 2-30µg/mL Protein Function Apoptotic suppressor. Has E3 ubiquitin-protein ligase activity. Mediates the proteasomal degradation of target proteins, such as caspase-3, SMAC or AIFM1. Inhibitor of caspase-3, -7 and -9. Mediates activation of MAP3K7/TAK1, leading to the activation of NF-kappa-B. |
| SDS-PAGE | 150 kDa |
|---|---|
| Purification Method | Antigen affinity purified + Protein A purified |
| Form | Liquid |
| Concentration | Lot by Lot |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Anti-XIAP antibody (Ab134877) - Western Blot
All lanes: Anti-XIAP antibody (Ab134877) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 56 kDa
Observed band size: 57 kDa
Exposure time: 0.9 s
Anti-XIAP antibody (Ab134877) - IF/ICC
FITC staining on IF
Samples: Human Hep G2 cells
Primary Ab: 20 µg/mL Anti-XIAP antibody (Ab134877)
Second Ab: 1 µg/mL FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
Anti-XIAP antibody (Ab134877) - IF/ICC
FITC staining on IF
Samples: Human MCF7 cells
Primary Ab: 20 μg/mL Anti-XIAP antibody (Ab134877)
Second Ab: 1.5 μg/mL FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
Anti-XIAP antibody (Ab134877) - IF/ICC
FITC staining on IF
Sample: MCF7 cells
Primary Ab: 20 µg/mL Anti-XIAP antibody (Ab134877)
Second Ab: 2 µg/mL FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
Anti-XIAP antibody (Ab134877) - IHC
DAB staining on IHC
Samples: Human Kidney Tissue
Primary Ab: 30 µg/mL Anti-XIAP antibody (Ab134877)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
Anti-XIAP antibody (Ab134877) - IHC
DAB staining on IHC
Samples: Human Breast cancer Tissue
Primary Ab: 30 µg/mL Anti-XIAP antibody (Ab134877)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
Anti-XIAP antibody (Ab134877) - IHC
DAB staining on IHC
Sample: Human Breast cancer Tissue
Primary Ab: 20 µg/mL Anti-XIAP antibody (Ab134877)
Control: Used PBS instead of primary antibody
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
Anti-XIAP antibody (Ab134877) - IHC
DAB staining on IHC
Sample: Human Kidney Tissue
Primary Ab: 20 µg/mL Anti-XIAP antibody (Ab134877)
Control: Used PBS instead of primary antibody
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
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| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
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| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
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| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
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| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
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| Application | Dilution info |
|---|---|
| WB | 0.01-3 µg/mL |
| IHC | 2-30 µg/mL |
| IF/ICC | 2-30µg/mL |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F1101811 | Certificate of Analysis | Nov 27, 2023 | Ab134877 |
| ZJ23F1101810 | Certificate of Analysis | Nov 27, 2023 | Ab134877 |
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| 56. Bertrand, Mathieu J M MJ and 8 more authors.. (2011) cIAP1/2 are direct E3 ligases conjugating diverse types of ubiquitin chains to receptor interacting proteins kinases 1 to 4 (RIP1-4).. PloS one, [PMID:21931591] |
| 57. Darding, M M and Meier, P P.. (2012) IAPs: guardians of RIPK1.. Cell death and differentiation, [PMID:22095281] |
| 58. Lewis, Eric M EM, Wilkinson, Amanda S AS, Davis, Nicole Y NY, Horita, David A DA and Wilkinson, John C JC.. (2011) Nondegradative ubiquitination of apoptosis inducing factor (AIF) by X-linked inhibitor of apoptosis at a residue critical for AIF-mediated chromatin degradation.. Biochemistry, (27): [PMID:22103349] |
| 59. Hanson, Alison J AJ and 5 more authors.. (2012) XIAP monoubiquitylates Groucho/TLE to promote canonical Wnt signaling.. Molecular cell, (9): [PMID:22304967] |
| 60. Edison, Natalia N and 11 more authors.. (2017) Degradation of Bcl-2 by XIAP and ARTS Promotes Apoptosis.. Cell reports, (10): [PMID:29020630] |
| 61. Koren, Elle and 8 more authors.. (2018) ARTS mediates apoptosis and regeneration of the intestinal stem cell niche.. Nature communications, (2): [PMID:30389919] |
