mAb(1A12); Mouse anti Human YAP1 Antibody; ELISA, WB, IHC, Flow; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab134985-10μl | 10μl | In stock | $69.90 | |
| Ab134985-50μl | 50μl | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $207.90 | |
| Ab134985-100μl | 100μl | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $327.90 | |
| Ab134985-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,907.90 |
mAb(1A12); Mouse anti Human YAP1 Antibody; ELISA, WB, IHC, Flow; Unconjugated
| Product Name | YAP1 Mouse mAb - Primary antibody, specific to YAP1, IgG1 |
|---|---|
| Synonyms | 65 kDa Yes-associated protein; COB1; Protein Yorkie Homolog; Transcriptional Coactivator YAP1; YAP; YAP1; YAP1-2gamma; YAP2; YAP2L; YAP65; YAP65yes-associated protein 2; Yes Associated Protein; Yes-associated protein 1; Yes-associated protein 1, 65kDa; ye |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, 1.0 mg/mL |
| Host species | Mouse |
| Specificity | YAP1 |
| Immunogen | Purified recombinant fragment of human YAP1 (AA: 250-447) expressed in E. Coli. |
| Positive Control | WB: HeLa, MCF7, A549 and C6 lysates. IHC: Prostate cancer tissue, Rectum cancer tissue. Flow: HeLa cells. |
| Conjugation | Unconjugated |
| Grade | Carrier Free, ExactAb™, Validated |
| Product Description | Mouse anti Human YAP1 Antibody, Monoclonal antibody, could be used for ELISA, WB, IHC, FC and so on. |
| Isotype | IgG1 |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein G purified |
| Form | Liquid |
| Concentration | 1.0 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles. |
YAP1 Mouse mAb (Ab134985) - Western Blot
All lanes: YAP1 Mouse mAb (Ab134985) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/50000 dilution
Predicted band size: 65 kDa
Observed band size: 65 kDa
YAP1 Mouse mAb (Ab134985) - IHC
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissues using YAP1 Mouse mAb (Ab134985) with DAB staining.
YAP1 Mouse mAb (Ab134985) - IHC
Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using YAP1 Mouse mAb (Ab134985) with DAB staining.
YAP1 Mouse mAb (Ab134985) - Flow Cytometry
Flow cytometric analysis of HeLa cells using YAP1 Mouse mAb (Ab134985) (green) and negative control (red).
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/2000 |
| ELISA |
1/10000
|
| IHC |
1/200-1/1000
|
Enter Lot Number to search for COA:
To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0404294 | Certificate of Analysis | Apr 09, 2024 | Ab134985 |
| ZJ23F0700708 | Certificate of Analysis | Jul 26, 2023 | Ab134985 |
| ZJ23F0700719 | Certificate of Analysis | Jul 26, 2023 | Ab134985 |
| 1. Todd D Taylor,Hideki Noguchi,Yasushi Totoki,Atsushi Toyoda,Yoko Kuroki,Ken Dewar,Christine Lloyd,Takehiko Itoh,Tadayuki Takeda,Dae-Won Kim,Xinwei She,Karen F Barlow,Toby Bloom,Elspeth Bruford,Jean L Chang,Christina A Cuomo,Evan Eichler,Michael G FitzGerald,David B Jaffe,Kurt LaButti,Robert Nicol,Hong-Seog Park,Christopher Seaman,Carrie Sougnez,Xiaoping Yang,Andrew R Zimmer,Michael C Zody,Bruce W Birren,Chad Nusbaum,Asao Fujiyama,Masahira Hattori,Jane Rogers,Eric S Lander,Yoshiyuki Sakaki. (2006-03-24) Human chromosome 11 DNA sequence and analysis including novel gene identification.. Nature, 440 ((7083)): (497-500). [PMID:16554811] |
| 2. Sharon Gauci,Andreas O Helbig,Monique Slijper,Jeroen Krijgsveld,Albert J R Heck,Shabaz Mohammed. (2009-05-06) Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.. Analytical chemistry, 81 ((11)): (4493-4501). [PMID:19413330] |
| 3. Jongshin Kim,Haiin Jo,Hyowon Hong,Min Hwan Kim,Jin Man Kim,June-Koo Lee,Won Do Heo,Joon Kim. (2015-04-08) Actin remodelling factors control ciliogenesis by regulating YAP/TAZ activity and vesicle trafficking.. Nature communications, 6 (6781-6781). [PMID:25849865] |
| 4. Zhang Z, Lin Z, Zhou Z, Shen HC, Yan SF, Mayweg AV, Xu Z, Qin N, Wong JC, Zhang Z, Rong Y, Fry DC, Hu T.. (2014) Structure-Based Design and Synthesis of Potent Cyclic Peptides Inhibiting the YAP-TEAD Protein-Protein Interaction.. ACS Med Chem Lett, 5 (9): (993-998). [PMID:25221655] |
| 5. Sudol, M M and 7 more authors.. (1995) Characterization of the mammalian YAP (Yes-associated protein) gene and its role in defining a novel protein module, the WW domain.. The Journal of biological chemistry, (16): [PMID:7782338] |
| 6. Chen, H I HI and 7 more authors.. (1997) Characterization of the WW domain of human yes-associated protein and its polyproline-containing ligands.. The Journal of biological chemistry, (4): [PMID:9202023] |
| 7. Pires, J R JR and 8 more authors.. (2001) Solution structures of the YAP65 WW domain and the variant L30 K in complex with the peptides GTPPPPYTVG, N-(n-octyl)-GPPPY and PLPPY and the application of peptide libraries reveal a minimal binding epitope.. Journal of molecular biology, (14): [PMID:11743730] |
| 8. Komuro, Akihiko A, Nagai, Makoto M, Navin, Nicholas E NE and Sudol, Marius M.. (2003) WW domain-containing protein YAP associates with ErbB-4 and acts as a co-transcriptional activator for the carboxyl-terminal fragment of ErbB-4 that translocates to the nucleus.. The Journal of biological chemistry, (29): [PMID:12807903] |
| 9. Hoshino, Masataka M and 17 more authors.. (2006) Transcriptional repression induces a slowly progressive atypical neuronal death associated with changes of YAP isoforms and p73.. The Journal of cell biology, (13): [PMID:16461361] |
| 10. Kulman, John D JD, Harris, Jeff E JE, Xie, Ling L and Davie, Earl W EW.. (2007) Proline-rich Gla protein 2 is a cell-surface vitamin K-dependent protein that binds to the transcriptional coactivator Yes-associated protein.. Proceedings of the National Academy of Sciences of the United States of America, (22): [PMID:17502622] |
| 11. Matsuoka, Shuhei S and 12 more authors.. (2007) ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage.. Science (New York, N.Y.), (25): [PMID:17525332] |
| 12. Paliwal, Preeti P, Radha, Vegesna V and Swarup, Ghanshyam G.. (2007) Regulation of p73 by Hck through kinase-dependent and independent mechanisms.. BMC molecular biology, (30): [PMID:17535448] |
| 13. Zhao, Bin B and 15 more authors.. (2007) Inactivation of YAP oncoprotein by the Hippo pathway is involved in cell contact inhibition and tissue growth control.. Genes & development, (1): [PMID:17974916] |
| 14. Hao, Yawei Y, Chun, Alex A, Cheung, Kevin K, Rashidi, Babak B and Yang, Xiaolong X.. (2008) Tumor suppressor LATS1 is a negative regulator of oncogene YAP.. The Journal of biological chemistry, (29): [PMID:18158288] |
| 15. Levy, Dan D, Adamovich, Yaarit Y, Reuven, Nina N and Shaul, Yosef Y.. (2008) Yap1 phosphorylation by c-Abl is a critical step in selective activation of proapoptotic genes in response to DNA damage.. Molecular cell, (15): [PMID:18280240] |
| 16. Zhao, Bin B and 11 more authors.. (2008) TEAD mediates YAP-dependent gene induction and growth control.. Genes & development, (15): [PMID:18579750] |
| 17. Mayya, Viveka V and 7 more authors.. (2009) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.. Science signaling, (18): [PMID:19690332] |
| 18. Zhao, Bin B, Li, Li L, Tumaneng, Karen K, Wang, Cun-Yu CY and Guan, Kun-Liang KL.. (2010) A coordinated phosphorylation by Lats and CK1 regulates YAP stability through SCF(beta-TRCP).. Genes & development, (1): [PMID:20048001] |
| 19. Li, Ze Z and 7 more authors.. (2010) Structural insights into the YAP and TEAD complex.. Genes & development, (1): [PMID:20123905] |
| 20. Tomlinson, V V and 5 more authors.. (2010) JNK phosphorylates Yes-associated protein (YAP) to regulate apoptosis.. Cell death & disease, [PMID:21364637] |
| 21. Rigbolt, Kristoffer T G KT and 9 more authors.. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.. Science signaling, (15): [PMID:21406692] |
| 22. Liu, X X and 6 more authors.. (2013) PTPN14 interacts with and negatively regulates the oncogenic function of YAP.. Oncogene, (7): [PMID:22525271] |
| 23. Gaffney, Christian J CJ and 11 more authors.. (2012) Identification, basic characterization and evolutionary analysis of differentially spliced mRNA isoforms of human YAP1 gene.. Gene, (10): [PMID:22939869] |
| 24. Yazicioglu, Mustafa N MN and 7 more authors.. (2013) Cellular localization and characterization of cytosolic binding partners for Gla domain-containing proteins PRRG4 and PRRG2.. The Journal of biological chemistry, (6): [PMID:23873930] |
| 25. Williamson, Kathleen A KA and 16 more authors.. (2014) Heterozygous loss-of-function mutations in YAP1 cause both isolated and syndromic optic fissure closure defects.. American journal of human genetics, (6): [PMID:24462371] |
| 26. Liu, Jie J and 13 more authors.. (2017) Loss of DLG5 promotes breast cancer malignancy by inhibiting the Hippo signaling pathway.. Scientific reports, (7): [PMID:28169360] |
| 27. Zhou, Beiyun B and 22 more authors.. (2018) Claudin-18-mediated YAP activity regulates lung stem and progenitor cell homeostasis and tumorigenesis.. The Journal of clinical investigation, (1): [PMID:29400695] |
| 28. Amisaki, Masataka M, Tsuchiya, Hiroyuki H, Sakabe, Tomohiko T, Fujiwara, Yoshiyuki Y and Shiota, Goshi G.. (2019) Identification of genes involved in the regulation of TERT in hepatocellular carcinoma.. Cancer science, [PMID:30447097] |
