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Z-VAD(OMe)-FMK - 98%, high purity , CAS No.187389-52-2

≥98%

CAS#: 187389-52-2
Formula: C₂₂H₃₀FN₃O₇
Molecular Weight: 467.49
PubChem CID: 5497174

Item Number

V275259

Grouped product items
SKU	Size	Availability	Price
V275259-1mg	1mg	In stock	$246.90
V275259-5mg	5mg	Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!	$1,107.90
V275259-10mg	10mg	In stock	$1,993.90
V275259-25mg	25mg	Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!	$4,484.90
V275259-50mg	50mg	In stock	$8,071.90
V275259-100mg	100mg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$14,529.90

Cell permeable, irreversible pan-caspase inhibitor

Basic Description

Synonyms	187389-52-2 \| Z-VAD(OMe)-FMK \| Z-Val-Ala-Asp(OMe)-FMK \| pan-caspase inhibitor \| C22H30FN3O7 \| ZVAD-FMK \| 5-Bromo-2 -deoxyuridine \| Z-Val-Ala-Asp(OMe)-fluoromethyl ketone \| Z-Val-Ala-Asp(OMe)-CH2F \| methyl (3S)-5-fluoro-3-[[(2S)-2-[[(2S)-3-methyl-2-(phenylmethoxycarbonylami
Specifications & Purity	≥98%
Biochemical and Physiological Mechanisms	A cell-permeable, irreversible, pan-caspase inhibitor. Inhibits caspase processing and apoptosis induction in tumor cells in vitro . Once in the cell, endogenous esterase activity hydrolyzes the methyl groups to form the biologically active form.Non-selec
Storage Temp	Store at -20°C,Desiccated
Shipped In	Ice chest + Ice pads
Note	Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one week. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour. Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
Product Description	Product Application： Z-VAD-FMK has been used: to induce mixed-lineage kinase domain-like (MLKL) phosphorylation (p-MLKL) in L929 cells to induce autophagy in Jurkat and K562 cells to revert killing effects of oxaliplatin or oxaliplatin combination with chloroquine (CQ) on HCT116 and SW620 cell lines as a caspase inhibitor to inhibit apoptosis and assess nuclear factor-kB, heat shock protein 70 (Hsp 70), proto-oncogene (cmyc) and tumor suppressor protein p53

Associated Targets(Human)

MAOA Tclin Monoamine oxidase A (11911 Activities)

Discover Target: MAOA

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

MAOB Tclin Monoamine oxidase B (8835 Activities)

Discover Target: MAOB

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

CASP3 Tchem Caspase-3 (3632 Activities)

Discover Target: CASP3

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Jurkat (10389 Activities)

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

HeLa (62764 Activities)

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

CAPN9 Tbio Calpain-9 (15 Activities)

Discover Target: CAPN9

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Names and Identifiers

IUPAC Name	methyl (3S)-5-fluoro-3-[[(2S)-2-[[(2S)-3-methyl-2-(phenylmethoxycarbonylamino)butanoyl]amino]propanoyl]amino]-4-oxopentanoate
INCHI	InChI=1S/C22H30FN3O7/c1-13(2)19(26-22(31)33-12-15-8-6-5-7-9-15)21(30)24-14(3)20(29)25-16(17(27)11-23)10-18(28)32-4/h5-9,13-14,16,19H,10-12H2,1-4H3,(H,24,30)(H,25,29)(H,26,31)/t14-,16-,19-/m0/s1
InChi Key	MIFGOLAMNLSLGH-QOKNQOGYSA-N
Canonical SMILES	CC(C)C(C(=O)NC(C)C(=O)NC(CC(=O)OC)C(=O)CF)NC(=O)OCC1=CC=CC=C1
Isomeric SMILES	C[C@@H](C(=O)N[C@@H](CC(=O)OC)C(=O)CF)NC(=O)[C@H](C(C)C)NC(=O)OCC1=CC=CC=C1
PubChem CID	5497174
Molecular Weight	467.49

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8 results found

Lot Number	Certificate Type	Date	Item
K2302103	Certificate of Analysis	Aug 22, 2024	V275259
G2321148	Certificate of Analysis	May 22, 2024	V275259
A2212152	Certificate of Analysis	Jul 12, 2023	V275259
A2212493	Certificate of Analysis	Oct 21, 2022	V275259
A2212495	Certificate of Analysis	Oct 21, 2022	V275259
A2212496	Certificate of Analysis	Oct 21, 2022	V275259
A2212498	Certificate of Analysis	Oct 21, 2022	V275259
A2212497	Certificate of Analysis	Dec 24, 2021	V275259

References

1. Lu Q et al.. (2018) A novel probe to assess cytosolic entry of exogenous proteins.. Nat Commun, 9 (3104). [PMID:30082832]
2. Pavlyukov MS et al.. (2018) Apoptotic Cell-Derived Extracellular Vesicles Promote Malignancy of Glioblastoma Via Intercellular Transfer of Splicing Factors.. Cancer Cell, 34 (119-135.e10). [PMID:29937354]
3. Mahalapbutr P et al.. (2019) Butoxy Mansonone G Inhibits STAT3 and Akt Signaling Pathways in Non-Small Cell Lung Cancers: Combined Experimental and Theoretical Investigations.. Cancers (Basel), 11 (4): [PMID:30925736]
4. Hoffmann S et al.. (2020) FAM111 protease activity undermines cellular fitness and is amplified by gain-of-function mutations in human disease.. EMBO Rep, 21 (10): (e50662). [PMID:32776417]
5. Suzuki R et al.. (2014) Genistein potentiates the antitumor effect of 5-Fluorouracil by inducing apoptosis and autophagy in human pancreatic cancer cells.. Anticancer Res, 34 (9): (4685-92). [PMID:25202045]
6. Peltzer N et al.. (2016) Holding RIPK1 on the Ubiquitin Leash in TNFR1 Signaling.. Trends Cell Biol, 26 (6): (445-461). [PMID:26877205]
7. Henning Y et al.. (2022) Hypoxia aggravates ferroptosis in RPE cells by promoting the Fenton reaction.. Cell Death Dis, 13 (7): (662). [PMID:35906211]
8. Mei S et al.. (2020) Immunopeptidomic Analysis Reveals That Deamidated HLA-bound Peptides Arise Predominantly from Deglycosylated Precursors.. Mol Cell Proteomics, 19 (7): (1236-1247). [PMID:32357974]
9. Peltzer N et al.. (2018) LUBAC is essential for embryogenesis by preventing cell death and enabling haematopoiesis.. Nature, 557 (7703): (112-117). [PMID:29695863]
10. Fan Z et al.. (2022) Neuronal MD2 induces long-term mental impairments in septic mice by facilitating necroptosis and apoptosis.. Front Pharmacol, 13 (884821). [PMID:36016572]
11. Jaru-Ampornpan P et al.. (2017) Porcine Epidemic Diarrhea Virus 3C-Like Protease-Mediated Nucleocapsid Processing: Possible Link to Viral Cell Culture Adaptability.. J Virol, 91 (2): [PMID:27807240]
12. Gain C et al.. (2020) Proteasomal inhibition triggers viral oncoprotein degradation via autophagy-lysosomal pathway.. PLoS Pathog, 16 (2): (e1008105). [PMID:32092124]
13. Nishi K et al.. (2017) ROS-induced cleavage of NHLRC2 by caspase-8 leads to apoptotic cell death in the HCT116 human colon cancer cell line.. Cell Death Dis, 8 (12): (3218). [PMID:29242562]
14. Lemke J et al.. (2014) Selective CDK9 inhibition overcomes TRAIL resistance by concomitant suppression of cFlip and Mcl-1.. Cell Death Differ, 21 (3): (491-502). [PMID:24362439]
15. Onofre J et al.. (2020) Testicular tissue cryopreservation is the preferred method to preserve spermatogonial stem cells prior to transplantation.. Reprod Biomed Online, 40 (2): (261-269). [PMID:32001160]
16. Qian L et al.. (2016) Two-Photon Enzymatic Probes Visualizing Sub-cellular/Deep-brain Caspase Activities in Neurodegenerative Models.. Sci Rep, 6 (26385). [PMID:27210613]
17. Onofre J et al.. (2018) What is the best protocol to cryopreserve immature mouse testicular cell suspensions?. Reprod Biomed Online, 37 (6-17). [PMID:29776850]

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